Multitissue assessment of in vivo postprandial intracellular lipid partitioning in rats using localized 1H-[13C] magnetic resonance spectroscopy

Journal article


Jonkers, Richard A. M., Geraedts, Tom R., Van Loon, Luc J.C., Nicolay, Klaas and Prompers, Jeanine J. 2012. Multitissue assessment of in vivo postprandial intracellular lipid partitioning in rats using localized 1H-[13C] magnetic resonance spectroscopy. Magnetic Resonance in Medicine. 68 (4), pp. 997 - 1006. https://doi.org/10.1002/mrm.23321
AuthorsJonkers, Richard A. M., Geraedts, Tom R., Van Loon, Luc J.C., Nicolay, Klaas and Prompers, Jeanine J.
Abstract

Excess accumulation of lipids in nonadipose tissues such asskeletal muscle and liver has been implicated in the develop-ment of obesity-related disorders, but the cause of this ec-topic lipid overload remains unknown. The aim of this studywas to determine in vivo postprandial lipid partitioning in ratskeletal muscle and liver, using localized1H-[13C] magneticresonance spectroscopy in combination with the oral admin-istration of13C-labeled lipids. Six rats were measured atbaseline and 5 and 24 h after administration of 400 mg[U-13C]-labeled algal lipids. Five hours after administration,fractional13C enrichments of the lipid pools in muscle andliver were increased 3.9-fold and 4.6-fold (P < 0.05), respec-tively, indicating that part of the ingested lipids had beentaken up by muscle and liver tissue. At 24 h, fractional13Cenrichments of muscle and liver lipids were decreased 1.6-fold and 2.2-fold (P < 0.05), respectively, compared with the 5h values. This can be interpreted as a depletion of13C-labeledlipids from the intracellular lipid pools as a consequence oflipid turnover. In conclusion, the novel application of1H-[13C]magnetic resonance spectroscopy in combination with theoral administration of13C-labeled lipids is applicable for thelongitudinal assessment of in vivo lipid partitioning betweenmultiple tissues. Magn Reson Med 68:997–1006, 2012.VC2011Wiley Periodicals, Inc.Key words: magnetic resonance spectroscopy; muscle lipids;liver lipids; carbon-13

Keywordsmagnetic resonance spectroscopy; muscle lipids; liver lipids; carbon-13
Year2012
JournalMagnetic Resonance in Medicine
Journal citation68 (4), pp. 997 - 1006
PublisherJohn Wiley & Sons Inc.
ISSN0740-3194
Digital Object Identifier (DOI)https://doi.org/10.1002/mrm.23321
Scopus EID2-s2.0-84866734722
Page range997 - 1006
Research GroupMary MacKillop Institute for Health Research
Place of publicationUnited States
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