Transparency microplates under impact

Journal article


Lau, Chun Yat, Roslan, Zulhanif, Cheong, Brandon Huey-Ping, Chua, Wei Seong, Liew, Oi Wah and Ng, Tuck Wah. (2014). Transparency microplates under impact. Journal of Colloid and Interface Science. 426, pp. 56 - 63. https://doi.org/10.1016/j.jcis.2014.03.048
AuthorsLau, Chun Yat, Roslan, Zulhanif, Cheong, Brandon Huey-Ping, Chua, Wei Seong, Liew, Oi Wah and Ng, Tuck Wah
Abstract

Transparency microplates enable biochemical analysis in resource-limited laboratories. During the process of transfer, the analytes tittered into the wells may undergo spillage from one well to another due to lateral impact. Sidelong impact tests conducted found the absence of non-linear effects (e.g., viscoelastic behavior) but high energy loss. Finite element simulations conducted showed that the rectangular plate holding the transparencies could undergo z-axis deflections when a normal component of the force was present despite constraints being used. High speed camera sequences confirmed this and also showed the asymmetrical z-axis deflection to cause the contact line closer to impact to displace first when the advancing condition was exceeded. Capillary waves were found to travel toward the contact line at the opposite end, where if the advancing contact angle condition was exceeded, also resulted in spreading. The presence of surface scribing was found to limit contact line movement better. With water drops dispensed on scribed transparencies, immunity from momentum change of up to 9.07 kgm/s on impact was possible for volumes of 40 μL. In the case of glycerol drops immunity from momentum change of up to 9.07 kgm/s on impact extended to volumes of 90 μL. The improved immunity of glycerol was attributed to its heightened dampening characteristics and its higher attenuation of capillary waves. Overall, scribed transparency microplates were able to better withstand spillage from accidental impact. Accidental impact was also found not to cause any detrimental effects on the fluorescence properties of enhanced green fluorescent protein samples tested.

Keywordsmicroplate; transparency; impact; contact angle
Year2014
JournalJournal of Colloid and Interface Science
Journal citation426, pp. 56 - 63
PublisherAcademic Press
ISSN0021-9797
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jcis.2014.03.048
Scopus EID2-s2.0-84899052219
Page range56 - 63
Research GroupSchool of Behavioural and Health Sciences
Publisher's version
File Access Level
Controlled
Grant IDARC/DP120100583
Place of publicationUnited States of America
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