The gut microbiota is a major regulator of androgen metabolism in intestinal contents
Journal article
Colldén, Hannah, Landin, Andreas, Wallenius, Ville, Elebring, Erik, Fändriks, Lars, Nilsson, Maria E., Ryberg, Henrik, Poutanen, Matti, Sjögren, Klara, Vandenput, Liesbeth and Ohlsson, Claes. (2019). The gut microbiota is a major regulator of androgen metabolism in intestinal contents. American Journal of Physiology - Endocrinology and Metabolism. 317(6), pp. E1182 - E1192. https://doi.org/10.1152/ajpendo.00338.2019
Authors | Colldén, Hannah, Landin, Andreas, Wallenius, Ville, Elebring, Erik, Fändriks, Lars, Nilsson, Maria E., Ryberg, Henrik, Poutanen, Matti, Sjögren, Klara, Vandenput, Liesbeth and Ohlsson, Claes |
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Abstract | Androgens exert important effects both in androgen-responsive tissues and in the intestinal tract. To determine the impact of the gut microbiota (GM) on intestinal androgen metabolism, we measured unconjugated (free) and glucuronidated androgen levels in intestinal contents from the small intestine, with a low bacterial density, and from cecum and colon, with a high bacterial density. Using a specific, sensitive gas chromatography-tandem mass spectrometry method, we detected high levels of glucuronidated testosterone (T) and dihydrotestosterone (DHT) in small intestinal content of mice of both sexes, whereas in the distal intestine we observed remarkably high levels of free DHT, exceeding serum levels by >20-fold. Similarly, in young adult men high levels of unconjugated DHT, >70-fold higher than in serum, were detected in feces. In contrast to mice with a normal GM composition, germ-free mice had high levels of glucuronidated T and DHT, but very low free DHT levels, in the distal intestine. These findings demonstrate that the GM is involved in intestinal metabolism and deglucuronidation of DHT and T, resulting in extremely high free levels of the most potent androgen, DHT, in the colonic content of young and healthy mice and men. |
Keywords | dihydrotestosterone; glucuronidation; gut bacteria; intracrinology; testosterone |
Year | 2019 |
Journal | American Journal of Physiology - Endocrinology and Metabolism |
Journal citation | 317 (6), pp. E1182 - E1192 |
Publisher | American Physiological Society |
ISSN | 0193-1849 |
Digital Object Identifier (DOI) | https://doi.org/10.1152/ajpendo.00338.2019 |
Scopus EID | 2-s2.0-85076195708 |
Open access | Open access |
Page range | E1182 - E1192 |
Research Group | Mary MacKillop Institute for Health Research |
Publisher's version | License |
Place of publication | United States of America |
https://acuresearchbank.acu.edu.au/item/86q58/the-gut-microbiota-is-a-major-regulator-of-androgen-metabolism-in-intestinal-contents
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