Toxicity estimates for diuron and atrazine for the tropical marine cnidarian exaiptasia pallida and in-hospite Symbiodinium spp. Using PAM chlorophyll-a fluorometry

Journal article


Howe, Pelli, Reichelt-Brushett, Amanda J., Clark, Malcolm and Seery, Cliff. (2017) Toxicity estimates for diuron and atrazine for the tropical marine cnidarian exaiptasia pallida and in-hospite Symbiodinium spp. Using PAM chlorophyll-a fluorometry. Journal of Photochemistry and Photobiology, B: Biology. 171, pp. 125 - 132. https://doi.org/10.1016/j.jphotobiol.2017.05.006
AuthorsHowe, Pelli, Reichelt-Brushett, Amanda J., Clark, Malcolm and Seery, Cliff
Abstract

Effective ecotoxicological risk assessments for herbicides in tropical marine environments are restricted by a lack of toxicity data, sensitive test methods and endpoints for relevant species, and this requires rectification. The symbiotic sea anemone Exaiptasia pallida is a suitable test species, representing the phylum Cnidaria and allowing for assessments of toxicological responses of both the animal host and in-hospite Symbiodinium spp. Pulse amplitude modulated (PAM) chlorophyll-a fluorometry is recognised as a valuable ecotoxicological tool, and here newly-developed test methods are presented using PAM fluorometry to measure herbicide effects on photosynthetic efficiency of in-hospite Symbiodinium spp. Additionally, measurements on healthy laboratory-reared E. pallida provide baseline data demonstrating the normal effective quantum yield (EQY) and the maximum electron transport rate (ETRm) for Symbiodinium spp. in the absence of herbicide stress. Concentration-dependant reductions in the EQY and ETRm occurred during diuron and atrazine exposures; a mean 48-h EC50 (effective concentration; 50%) of 8 μg/L of diuron was estimated, however atrazine elicited a much lower toxicity. Twelve-day exposures to 10–200 μg/L diuron showed that the greatest EQY effect occurred during the first 48 h, with little subsequent change. However, longer exposures to the lowest diuron treatment (1 μg/L) showed the lowest EQYs after 96 h followed by recovery to control levels within 12 d. Furthermore, asexual reproduction was inhibited during 12-d exposures to diuron, and 12-d EC50 values of 100 and 132 μg/L were estimated to inhibit successful reproduction of pedal lacerates and juveniles by 50% respectively. This study provides much needed data contributions to species sensitivity curves for development of diuron and atrazine water quality guidelines in tropical marine environments.

Year2017
JournalJournal of Photochemistry and Photobiology, B: Biology
Journal citation171, pp. 125 - 132
PublisherElsevier
ISSN1011-1344
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jphotobiol.2017.05.006
Scopus EID2-s2.0-85019129547
Page range125 - 132
Research GroupSchool of Behavioural and Health Sciences
Place of publicationNetherlands
EditorsR. Carpentier, D. Ramaiah and A.L.B. Pinheiro
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https://acuresearchbank.acu.edu.au/item/86zv0/toxicity-estimates-for-diuron-and-atrazine-for-the-tropical-marine-cnidarian-exaiptasia-pallida-and-in-hospite-symbiodinium-spp-using-pam-chlorophyll-a-fluorometry

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