Myofibrillar and mitochondrial protein synthesis rates do not differ in young men following the ingestion of carbohydrate with whey, soy, or leucine-enriched soy protein after concurrent resistance-and endurance-type exercise

Journal article


Churchward-Venne, Tyler A., Pinckaers, Philippe J. M., Smeets, Joey S. J., Peeters, Wouter M., Zorenc, Antoine H., Schierbeek, Henk, Rollo, Ian, Verdijk, Lex B. and van Loon, Luc J. C.. (2019). Myofibrillar and mitochondrial protein synthesis rates do not differ in young men following the ingestion of carbohydrate with whey, soy, or leucine-enriched soy protein after concurrent resistance-and endurance-type exercise. The Journal of Nutrition. 149(2), pp. 210 - 220. https://doi.org/10.1093/jn/nxy251
AuthorsChurchward-Venne, Tyler A., Pinckaers, Philippe J. M., Smeets, Joey S. J., Peeters, Wouter M., Zorenc, Antoine H., Schierbeek, Henk, Rollo, Ian, Verdijk, Lex B. and van Loon, Luc J. C.
Abstract

Background Protein ingestion during recovery from resistance-type exercise increases postexercise muscle protein synthesis rates. Whey protein has been reported to have greater anabolic properties than soy protein, an effect which may be attributed to the higher leucine content of whey. Objective The objective of this study was to compare postprandial myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates after ingestion of carbohydrate with whey, soy, or soy protein enriched with free leucine (to match the leucine content of whey) during recovery from a single bout of concurrent resistance- and endurance-type exercise in young healthy men. Methods In a randomized, double-blind, parallel-group design, 36 healthy young recreationally active men (mean ± SEM age: 23 ± 0.4 y) received a primed continuous infusion of L-[ring-13C6]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 45 g carbohydrate with 20 g protein from whey (WHEY), soy (SOY), or leucine-enriched soy (SOY + LEU) after concurrent resistance- and endurance-type exercise. Blood and muscle biopsies were collected over a 360 min postexercise recovery period to assess MyoPS and MitoPS rates, and associated signaling through the mammalian target of rapamycin complex 1 (mTORC1). Results Postprandial peak plasma leucine concentrations were significantly higher in WHEY (mean ± SEM: 322 ± 10 μmol/L) and SOY + LEU (328 ± 14 μmol/L) compared with SOY (216 ± 6 μmol/L) (P < 0.05). Despite the apparent differences in plasma leucinemia, MyoPS (WHEY: 0.054 ± 0.002; SOY: 0.053 ± 0.004; SOY + LEU: 0.056 ± 0.004%·h−1; P = 0.83), and MitoPS (WHEY: 0.061 ± 0.004; SOY: 0.061 ± 0.006; SOY + LEU: 0.063 ± 0.004%·h−1; P = 0.96) rates over the entire 360 min recovery period did not differ between treatments. Similarly, signaling through mTORC1Ser2448, p70S6kThr389, 4E-BP1Thr37/46, and rpS6Ser235/236 was similar between treatments. Conclusion Postexercise MyoPS and MitoPS rates do not differ after co-ingestion of carbohydrate with 20 g protein from whey, soy, or leucine-enriched soy protein during 360 min of recovery from concurrent resistance- and endurance-type exercise in young, recreationally active men. This trial was registered at Nederlands Trial Register as NTR5098.

Keywordsmuscle protein synthesis; young men; carbohydrate; dietary protein; whey; soy; leucine; concurrent exercise
Year2019
JournalThe Journal of Nutrition
Journal citation149 (2), pp. 210 - 220
PublisherOxford University Press
ISSN0022-3166
Digital Object Identifier (DOI)https://doi.org/10.1093/jn/nxy251
Scopus EID2-s2.0-85062290184
Open accessOpen access
Page range210 - 220
Research GroupMary MacKillop Institute for Health Research
Publisher's version
Place of publicationUnited States of America
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