The effect of nitrate supplementation on muscle contraction in healthy adults

Journal article


Hoon, Matthew W., Fornusek, Che, Chapman, Phillip Grant and Johnson, Nathan A.. (2016) The effect of nitrate supplementation on muscle contraction in healthy adults. European Journal of Sport Science. 15(8), pp. 712 - 719. https://doi.org/10.1080/17461391.2015.1053418
AuthorsHoon, Matthew W., Fornusek, Che, Chapman, Phillip Grant and Johnson, Nathan A.
Abstract

This study examined the effect of dietary supplementation with inorganic nitrate ( ) on markers of contractile function in human knee extensors. In a double-blinded, randomized cross-over design, 18 (12 M) healthy participants undertook four days of supplementation with either nitrate-rich beetroot juice (NITRATE; days 1–3: 525 mg , day 4: 1050 mg ) or nitrate-depleted beetroot juice (PLACEBO). On the fourth day, isometric knee extension force was assessed during a series of voluntary and electrically evoked (stimulation) tests. In addition, muscular fatigue was examined in two separate continuous-stimulation (0.8 s tetanus with a 1:1 work:rest ratio for 102.4 s) fatigue tests, one with and one without blood flow restriction. There were no differences for maximum voluntary contraction, peak twitch force, half-relaxation time and the force–frequency relationship for stimulations up to 100 Hz between the NITRATE and PLACEBO trials. No differences between trials were observed in the non-restricted fatigue test, however NITRATE was found to attenuate the decline in force during the restricted test, such that the force at the 80 s mark (PLACEBO: 66 ± 11 vs. NITRATE 74 ± 9% of initial force; P = .01) and 102 s mark (PLACEBO: 47 ± 8% vs. NITRATE 55 ± 8%; P < .01) were significantly higher. These results suggest that four days of supplementation elicits peripheral responses in muscle that attenuate muscular fatigue during exhaustive exercise under hypovolemic conditions. This ergogenic action is likely attributable to improved Ca2+ handling in the muscle, or enhanced perfusion during ischemia.

KeywordsBeetroot juice; excitation–contraction coupling; fatigue; muscle force; transcutaneous electrical nerve stimulation
Year2016
JournalEuropean Journal of Sport Science
Journal citation15 (8), pp. 712 - 719
PublisherTaylor & Francis
ISSN1746-1391
Digital Object Identifier (DOI)https://doi.org/10.1080/17461391.2015.1053418
Scopus EID2-s2.0-84939167229
Page range712 - 719
Research GroupSports Performance, Recovery, Injury and New Technologies (SPRINT) Research Centre
Place of publicationUnited Kingdom
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