The effects of knee injury on skeletal muscle function, Na+-ATPase content, and isoform abundance

Journal article


Perry, Ben D., Levinger, P, Morris, Hayden G., Petersen, Aaron C., Garnham, Andrew, Levinger, Itamar and McKenna, Michael J.. (2015) The effects of knee injury on skeletal muscle function, Na+-ATPase content, and isoform abundance. Physiological Reports. 3(2), pp. 1 - 14. https://doi.org/10.14814/phy2.12294
AuthorsPerry, Ben D., Levinger, P, Morris, Hayden G., Petersen, Aaron C., Garnham, Andrew, Levinger, Itamar and McKenna, Michael J.
Abstract

While training upregulates skeletal muscle Na+, K+‐ATPase (NKA), the effects of knee injury and associated disuse on muscle NKA remain unknown. This was therefore investigated in six healthy young adults with a torn anterior cruciate ligament, (KI; four females, two males; age 25.0 ± 4.9 years; injury duration 15 ± 17 weeks; mean ± SD) and seven age‐ and BMI‐matched asymptomatic controls (CON; five females, two males). Each participant underwent a vastus lateralis muscle biopsy, on both legs in KI and one leg in CON. Muscle was analyzed for muscle fiber type and cross‐sectional area (CSA), NKA content ([3H]ouabain binding), and α1–3 and β1–2 isoform abundance. Participants also completed physical activity and knee function questionnaires (KI only); and underwent quadriceps peak isometric strength, thigh CSA and postural sway assessments in both injured and noninjured legs. NKA content was 20.1% lower in the knee‐injured leg than the noninjured leg and 22.5% lower than CON. NKA α2 abundance was 63.0% lower in the knee‐injured leg than the noninjured leg, with no differences in other NKA isoforms. Isometric strength and thigh CSA were 21.7% and 7.1% lower in the injured leg than the noninjured leg, respectively. In KI, postural sway did not differ between legs, but for two‐legged standing was 43% higher than CON. Hence, muscle NKA content and α2 abundance were reduced in severe knee injury, which may contribute to impaired muscle function. Restoration of muscle NKA may be important in rehabilitation of muscle function after knee and other lower limb injury.

Keywordsdisuse; excitability; sodium potassium pump
Year2015
JournalPhysiological Reports
Journal citation3 (2), pp. 1 - 14
PublisherAmerican Physiological Society
ISSN2051-817X
Digital Object Identifier (DOI)https://doi.org/10.14814/phy2.12294
Scopus EID2-s2.0-84974597779
Open accessOpen access
Page range1 - 14
Publisher's version
Additional information

© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Place of publicationUnited Kingdom
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