Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise

Journal article


Koopman, René, Gleeson, Benjamin G., Gijsen, Annemie P., Groen, Bart B. L., Senden, Joan M. G., Rennie, Michael J. and van Loon, Luc J. C. 2011. Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise. European Journal of Applied Physiology. 111 (8), pp. 1871 - 1878. https://doi.org/10.1007/s00421-010-1808-9
AuthorsKoopman, René, Gleeson, Benjamin G., Gijsen, Annemie P., Groen, Bart B. L., Senden, Joan M. G., Rennie, Michael J. and van Loon, Luc J. C.
Abstract

We examined the effect of an acute bout of resistance exercise on fractional muscle protein synthesis rates in human type I and type II muscle fibres. After a standardised breakfast (31 ± 1 kJ kg−1 body weight, consisting of 52 Energy% (En%) carbohydrate, 34 En% protein and 14 En% fat), 9 untrained men completed a lower-limb resistance exercise bout (8 sets of 10 repetitions leg press and leg extension at 70% 1RM). A primed, continuous infusion of l-[ring-13C6]phenylalanine was combined with muscle biopsies collected from both legs immediately after exercise and after 6 h of post-exercise recovery. Single muscle fibres were dissected from freeze-dried biopsies and stained for ATPase activity with pre-incubation at a pH of 4.3. Type I and II fibres were separated under a light microscope and analysed for protein-bound l-[ring-13C6]phenylalanine labelling. Baseline (post-exercise) l-[ring-13C6]phenylalanine muscle tissue labelling, expressed as (∂13C/12C), averaged −32.09 ± 0.28, −32.53 ± 0.10 and −32.02 ± 0.16 in the type I and II muscle fibres and mixed muscle, respectively (P = 0.14). During post-exercise recovery, muscle protein synthesis rates were marginally (8 ± 2%) higher in the type I than type II muscle fibres, at 0.100 ± 0.005 versus 0.094 ± 0.005%/h, respectively (P < 0.05), whereby rates of mixed muscle protein were 0.091 ± 0.005%/h. Muscle protein synthesis rates following resistance-type exercise are only marginally higher in type I compared with type II muscle fibres.

Keywordsskeletal muscle; muscle fibre type; ATPase; FSR; stable isotopes
Year2011
JournalEuropean Journal of Applied Physiology
Journal citation111 (8), pp. 1871 - 1878
PublisherSpringer
ISSN1439-6319
Digital Object Identifier (DOI)https://doi.org/10.1007/s00421-010-1808-9
Scopus EID2-s2.0-80053911646
Open accessOpen access
Page range1871 - 1878
Research GroupMary MacKillop Institute for Health Research
Publisher's version
Place of publicationGermany
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