Effectors of the mammalian plasma membrane NADH-oxidoreductase system. Short-chain ubiquinone analogues as potent stimulators
Journal article
Vaillant, Francois, Larm, Jari, McMullen, Gabrielle, Wolvetang, Ernst and Lawen, Alfons. (1996). Effectors of the mammalian plasma membrane NADH-oxidoreductase system. Short-chain ubiquinone analogues as potent stimulators. Journal of Bioenergetics and Biomembranes. 28(6), pp. 531 - 540. https://doi.org/10.1007/BF02110443
Authors | Vaillant, Francois, Larm, Jari, McMullen, Gabrielle, Wolvetang, Ernst and Lawen, Alfons |
---|---|
Abstract | In the presence of effectors variations in the two recognized activities of the plasma membrane NADH-oxidoreductase system were studied in separate, specificin vitro assays. We report here that ubiquinone analogues that contain a short, less hydrophobic side chain than coenzyme Q-10 dramatically stimulate the NADH-oxidase activity of isolated rat liver plasma membranes whereas they show no effect on the reductase activity of isolated membranes. If measured in assays of the NADH∶ferricyanide reductase of living cultured cells these compounds have only a limited effect; the oxidase activity of whole cells is not measurable in our hands. We have furthermore identified selective inhibitors of both enzyme activities. In particular, the NADH-oxidase activity can be significantly inhibited by structural analogues of ubiquinone, such as capsaicin and resiniferatoxin. The NADH∶ferricyanide reductase, on the other hand, is particularly sensitive to pCMBS, indicating the presence of a sulfhydryl group or groups at its active site. The identification of these specific effectors of the different enzyme activities of the PMOR yields further insights into the function of this system. |
Keywords | Plasma membrane NADH-oxidoreductase; NADH-oxidase; NADH∶ferricyanide reductase; ubiquinone analogues; ρ0 cells |
Year | 1996 |
Journal | Journal of Bioenergetics and Biomembranes |
Journal citation | 28 (6), pp. 531 - 540 |
Publisher | Springer |
ISSN | 0145-479X |
Digital Object Identifier (DOI) | https://doi.org/10.1007/BF02110443 |
Scopus EID | 2-s2.0-0029966791 |
Page range | 531 - 540 |
Publisher's version | File Access Level Controlled |
Place of publication | Germany |
https://acuresearchbank.acu.edu.au/item/87x0x/effectors-of-the-mammalian-plasma-membrane-nadh-oxidoreductase-system-short-chain-ubiquinone-analogues-as-potent-stimulators
Restricted files
Publisher's version
161
total views0
total downloads8
views this month0
downloads this month