Effectors of the mammalian plasma membrane NADH-oxidoreductase system. Short-chain ubiquinone analogues as potent stimulators

Journal article


Vaillant, Francois, Larm, Jari, McMullen, Gabrielle, Wolvetang, Ernst and Lawen, Alfons. (1996) Effectors of the mammalian plasma membrane NADH-oxidoreductase system. Short-chain ubiquinone analogues as potent stimulators. Journal of Bioenergetics and Biomembranes. 28(6), pp. 531 - 540. https://doi.org/10.1007/BF02110443
AuthorsVaillant, Francois, Larm, Jari, McMullen, Gabrielle, Wolvetang, Ernst and Lawen, Alfons
Abstract

In the presence of effectors variations in the two recognized activities of the plasma membrane NADH-oxidoreductase system were studied in separate, specificin vitro assays. We report here that ubiquinone analogues that contain a short, less hydrophobic side chain than coenzyme Q-10 dramatically stimulate the NADH-oxidase activity of isolated rat liver plasma membranes whereas they show no effect on the reductase activity of isolated membranes. If measured in assays of the NADH∶ferricyanide reductase of living cultured cells these compounds have only a limited effect; the oxidase activity of whole cells is not measurable in our hands. We have furthermore identified selective inhibitors of both enzyme activities. In particular, the NADH-oxidase activity can be significantly inhibited by structural analogues of ubiquinone, such as capsaicin and resiniferatoxin. The NADH∶ferricyanide reductase, on the other hand, is particularly sensitive to pCMBS, indicating the presence of a sulfhydryl group or groups at its active site. The identification of these specific effectors of the different enzyme activities of the PMOR yields further insights into the function of this system.

KeywordsPlasma membrane NADH-oxidoreductase; NADH-oxidase; NADH∶ferricyanide reductase; ubiquinone analogues; ρ0 cells
Year1996
JournalJournal of Bioenergetics and Biomembranes
Journal citation28 (6), pp. 531 - 540
PublisherSpringer
ISSN0145-479X
Digital Object Identifier (DOI)https://doi.org/10.1007/BF02110443
Scopus EID2-s2.0-0029966791
Page range531 - 540
Place of publicationGermany
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