Global impact of heparin on gene expression profiles in neural cells infected by Enterovirus 71

Journal article


Pourianfar, H. R., Palombo, E. and Grollo, L.. (2014). Global impact of heparin on gene expression profiles in neural cells infected by Enterovirus 71. Intervirology: international journal of basic and medical virology. 57(2), pp. 93 - 100. https://doi.org/10.1159/000355872
AuthorsPourianfar, H. R., Palombo, E. and Grollo, L.
Abstract

Objectives: Heparan sulphate mimetics, particularly heparin (Hep), have previously been shown to considerably inhibit infection of enterovirus 71 (EV71) in Vero cells. Therefore, in this study, a genome-wide DNA microarray was performed to gain insight into the mechanism(s) of action of Hep against infection of a human neural cell line, SK-N-SH, with a clinical strain of EV71. Methods: This study focused on a selection of EV71-induced genes whose expression profiling was exclusively affected by the antiviral activity of Hep. The selection procedure was performed through a statistical multi-level comparison with the following controls: negative control cells, compound control (cells treated with Hep only), virus control (cells treated with virus only) and treatment control (EV71-infected cells treated with Hep). Results: Overall, of more than 30,000 genes studied, 14 well-known annotated genes were selected that may be targets for the antiviral activity of Hep against EV71 infection in neural cells. For most of these genes, Hep appeared to modulate the impact of EV71 infection on the expression pattern of the genes. Conclusions: The findings of this research may provide initial assistance in new directions for studies to design molecular drug targets against EV71 infection.

Year2014
JournalIntervirology: international journal of basic and medical virology
Journal citation57 (2), pp. 93 - 100
ISSN0300-5526
Digital Object Identifier (DOI)https://doi.org/10.1159/000355872
Page range93 - 100
Research GroupSchool of Behavioural and Health Sciences
Publisher's version
File Access Level
Controlled
Permalink -

https://acuresearchbank.acu.edu.au/item/87zv8/global-impact-of-heparin-on-gene-expression-profiles-in-neural-cells-infected-by-enterovirus-71

Restricted files

Publisher's version

  • 65
    total views
  • 0
    total downloads
  • 1
    views this month
  • 0
    downloads this month
These values are for the period from 19th October 2020, when this repository was created.

Export as

Related outputs

Salivary blockade protects the lower respiratory tract of mice from lethal influenza virus infection
Ivinson, Karen, Deliyannis, Georgia, McNabb, Leanne, Grollo, Lara, Gilbertson, Brad, Jackson, David and Brown, Lorena E.. (2017). Salivary blockade protects the lower respiratory tract of mice from lethal influenza virus infection. Journal of Virology. 91(14), pp. 1 - 12. https://doi.org/10.1128/JVI.00624-17
Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
Ramanathan, Babu, Poh, Chit L., Kirk, Kristin, McBride, William John Hannan, Aaskov, John and Grollo, Lara. (2016). Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLoS ONE. 11(5), pp. 1 - 22. https://doi.org/10.1371/journal.pone.0155900
Enterovirus-specific anti-peptide antibodies
Poh, Chit Laa, Kirk, Katherine, Chua, Hui Na and Grollo, Lara. (2015). Enterovirus-specific anti-peptide antibodies. In In Houen, Gunnar (Ed.). Peptide antibodies methods and protocols pp. 341-350 Humana Press. https://doi.org/10.1007/978-1-4939-2999-3_29
Development of antiviral agents toward enterovirus 71 infection
Pourianfar, Hamid Reza and Grollo, Lara. (2015). Development of antiviral agents toward enterovirus 71 infection. Journal of Microbiology Immunology and Infection. 48(1), pp. 1 - 8. https://doi.org/10.1016/j.jmii.2013.11.011
Evidence of synergistic activity of medicinal plant extracts against neuraminidase inhibitor resistant strains of influenza
Rajasekaran, Dhivya, Palombo, Enzo, Yeo, Tiong Chia, Ley, Diana, Tu, Chu Lee, Malherbe, Francois and Grollo, Lara. (2014). Evidence of synergistic activity of medicinal plant extracts against neuraminidase inhibitor resistant strains of influenza. Advances in Microbiology. 4(16), pp. 1260 - 1277. https://doi.org/10.4236/aim.2014.416136
Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface hepaan sulphate
Pourianfar, Hamid Reza, Kirk, Kristin and Grollo, Lara. (2014). Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface hepaan sulphate. VirusDisease. 25(3), pp. 277 - 284. https://doi.org/10.1007/s13337-013-0172-x
Identification of traditional medicinal plant extracts with novel anti-influenza activity
Rajasekaran, Dhivya, Palombo, Enzo, Yeo, Tiong Chia, Ley, Diana, Tu, Chu, Malherbe, Francois and Grollo, Lara. (2013). Identification of traditional medicinal plant extracts with novel anti-influenza activity. PLoS One (online). 8(11), pp. 1 - 15. https://doi.org/10.1371/journal.pone.0079293
In vitro evaluation of the antiviral activity of heparan sulfate mimetic compounds against Enterovirus 71
Pourianfar, Hamid, Poh, Chit Laa, Fecondo, John and Grollo, Lara. (2012). In vitro evaluation of the antiviral activity of heparan sulfate mimetic compounds against Enterovirus 71. Virus Research. 169(1), pp. 22 - 29. https://doi.org/10.1016/j.virusres.2012.06.025
A colorimetric-based accurate method for the determination of enterovirus 71 titer
Pourianfar, Hamid Reza, Javadi, Arman and Grollo, Lara. (2012). A colorimetric-based accurate method for the determination of enterovirus 71 titer. Indian Journal of Virology. 23(3), pp. 303 - 310. https://doi.org/10.1007/s13337-012-0105-0
Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach
Kirk, Kristin, Poh, Chit Lau, Fecondo, John, Shaw, Jillian and Grollo, Lara. (2012). Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach. Vaccine. 30(49), pp. 7105 - 7110. https://doi.org/10.1016/j.vaccine.2012.09.030