Hematopoietic AMPK beta1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity

Journal article


Galic, Sandra, Fullerton, Morgan, Schertzer, Jonathan, Sikkema, Sarah, Marcinko, Katarina, Walkley, Carl, Izon, David, Honeyman, Jane, Chen, Zhi-Ping, Van Denderen, Bryce, Kemp, Bruce and Steinberg, Gregory. (2011). Hematopoietic AMPK beta1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. Journal of Clinical Investigation. 121(12), pp. 4903 - 4915. https://doi.org/10.1172/JCI58577
AuthorsGalic, Sandra, Fullerton, Morgan, Schertzer, Jonathan, Sikkema, Sarah, Marcinko, Katarina, Walkley, Carl, Izon, David, Honeyman, Jane, Chen, Zhi-Ping, Van Denderen, Bryce, Kemp, Bruce and Steinberg, Gregory
Abstract

Individuals who are obese are frequently insulin resistant, putting them at increased risk of developing type 2 diabetes and its associated adverse health conditions. The accumulation in adipose tissue of macrophages in an inflammatory state is a hallmark of obesity-induced insulin resistance. Here, we reveal a role for AMPK β1 in protecting macrophages from inflammation under high lipid exposure. Genetic deletion of the AMPK β1 subunit in mice (referred to herein as β1–/– mice) reduced macrophage AMPK activity, acetyl-CoA carboxylase phosphorylation, and mitochondrial content, resulting in reduced rates of fatty acid oxidation. β1–/– macrophages displayed increased levels of diacylglycerol and markers of inflammation, effects that were reproduced in WT macrophages by inhibiting fatty acid oxidation and, conversely, prevented by pharmacological activation of AMPK β1–containing complexes. The effect of AMPK β1 loss in macrophages was tested in vivo by transplantation of bone marrow from WT or β1–/– mice into WT recipients. When challenged with a high-fat diet, mice that received β1–/– bone marrow displayed enhanced adipose tissue macrophage inflammation and liver insulin resistance compared with animals that received WT bone marrow. Thus, activation of AMPK β1 and increasing fatty acid oxidation in macrophages may represent a new therapeutic approach for the treatment of insulin resistance.

Year2011
JournalJournal of Clinical Investigation
Journal citation121 (12), pp. 4903 - 4915
ISSN0021-9738
Digital Object Identifier (DOI)https://doi.org/10.1172/JCI58577
Page range4903 - 4915
Publisher's version
File Access Level
Controlled
Permalink -

https://acuresearchbank.acu.edu.au/item/88050/hematopoietic-ampk-beta1-reduces-mouse-adipose-tissue-macrophage-inflammation-and-insulin-resistance-in-obesity

Restricted files

Publisher's version

  • 132
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month
These values are for the period from 19th October 2020, when this repository was created.

Export as

Related outputs

The AMPK activator ATX-304 alters cellular metabolism to protect against cisplatin-induced acute kidney injury
Katerelos, Marina, Gleich, Kurt, Harley, Geoff, Loh, Kim, Oakhill, Jonathan, Kemp, Bruce Ernest, De Souza, David P., Narayana, Vinod K., Coughlan, Melinda T., Laskowski, Adrienne, Ling, Naomi, Murray-Segal, Lisa, Brink, Robert, Lee, Mardiana, Power, David and Mount, Peter. (2024). The AMPK activator ATX-304 alters cellular metabolism to protect against cisplatin-induced acute kidney injury. Biomedicine and Pharmacotherapy. 175, pp. 116730-116742. https://doi.org/10.1016/j.biopha.2024.116730
An AMPKa2-specific phospho-switch controls lysosomal targeting for activation
Morrison, Kaitlin R., Smiles, William J., Ling, Naomi X. Y., Hoque, Ashfaqul, Shea, Gabrielle, Ngoei, Kevin R. W., Yu, Dingyi, Murray-Segal, Lisa, Scott, John W., Galic, Sandra, Kemp, Bruce E., Petersen, Janni and Oakhill, Jonathan S.. (2022). An AMPKa2-specific phospho-switch controls lysosomal targeting for activation. Cell Reports. 38(7), p. Article 110365. https://doi.org/10.1016/j.celrep.2022.110365
Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis
Neopane, Katyayanee, Kozlov, Natalie, Negoita, Florentina, Murray-Segal, Lisa, Brink, Robert, Hoque, Ashfaqul, Ovens, Ashley J., Tjin, Gavin, McAloon, Luke M., Yu, Dingyi, Ling, Naomi X. Y., Sanders, Matthew J., Oakhill, Jonathan S., Scott, John W., Steinberg, Gregory R., Loh, Kim, Kemp, Bruce E., Sakamoto, Kei and Galic, Sandra. (2022). Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis. Cell Reports. 41(12), pp. 1-16. https://doi.org/10.1016/j.celrep.2022.111862
ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis
Zhang, Ting, Yin, Chaoran, Fedorov, Aleksandr, Qiao, Liangjun, Bao, Hongliang, Beknazarov, Nazar, Wang, Shiyu, Gautam, Avishekh, Williams, Riley M., Crawford, Jeremy Chase, Peri, Suraj, Studitsky, Vasily, Beg, Amer A., Thomas, Paul G., Walkley, Carl, Xu, Yan, Poptsova, Maria, Herbert, Alan and Balachandran, Siddharth. (2022). ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis. Nature. 606, pp. 594-602. https://doi.org/10.1038/s41586-022-04753-7
Structure-function analysis of the AMPK activator SC4 and identification of a potent pan AMPK activator
Ovens, Ashley J., Gee, Yi Sing, Ling, Naomi X. Y., Yu, Dingyi, Hardee, Justin P., Chung, Jin D., Ngoei, Kevin R. W., Waters, Nicholas J., Hoffman, Nolan J., Scott, John W., Loh, Kim, Spengler, Katrin, Heller, Regine, Parker, Michael W., Lynch, Gordon S., Huang, Fei, Galic, Sandra, Kemp, Bruce E., Baell, Jonathan B., ... Langendorf, Christopher G.. (2022). Structure-function analysis of the AMPK activator SC4 and identification of a potent pan AMPK activator. Biochemical Journal. 479(11), pp. 1181-1204. https://doi.org/10.1042/BCJ20220067
Calcium/calmodulin-dependent protein kinase kinase 2 regulates hepatic fuel metabolism
Stork, Britanny A., Dean, Adam, Ortiz, Andrea R., Saha, Pradip, Putluri, Nagireddy, Planas-Silva, Maricarmen D., Mahmud, Iqbal, Rajapakshe, Kimal, Coarfa, Cristian, Knapp, Stefan, Lorenzi, Philip L., Kemp, Bruce E., Turk, Benjamin E., Scott, John W., Means, Anthony R. and York, Brian. (2022). Calcium/calmodulin-dependent protein kinase kinase 2 regulates hepatic fuel metabolism. Molecular Metabolism. 62, p. Article 101513. https://doi.org/10.1016/j.molmet.2022.101513
Defective AMPK regulation of cholesterol metabolism accelerates atherosclerosis by promoting HSPC mobilization and myelopoiesis
Lee, Man K. S., Cooney, Olivia D., Lin, Xuzhu, Nadarajah, Shaktypreya, Dragoljevic, Dragana, Huynh, Kevin, Onda, Danise-Ann, Galic, Sandra, Meikle, Peter J., Edlund, Thomas, Fullerton, Morgan D., Kemp, Bruce E., Murphy, Andrew J. and Loh, Kim. (2022). Defective AMPK regulation of cholesterol metabolism accelerates atherosclerosis by promoting HSPC mobilization and myelopoiesis. Molecular Metabolism. 61, p. Article 101514. https://doi.org/10.1016/j.molmet.2022.101514
Genome-wide screening identifies cell-cycle control as a synthetic lethal pathway with SRSF2P95H mutation
Xu, Jane Jialu, Chalk, Alistair M., Nikolic, Iva, Simpson, Kaylene J., Smeets, Monique F. and Walkley, Carl R.. (2022). Genome-wide screening identifies cell-cycle control as a synthetic lethal pathway with SRSF2P95H mutation. Blood Advances. 6(7), pp. 2092-2106. https://doi.org/10.1182/bloodadvances.2021004571
Disrupting AMPK-glycogen binding in mice increases carbohydrate utilization and reduces exercise capacity
Janzen, Natalie R., Whitfield, Jamie, Murray-Segal, Lisa, Kemp, Bruce E., Hawley, John A. and Hoffman, Nolan J.. (2022). Disrupting AMPK-glycogen binding in mice increases carbohydrate utilization and reduces exercise capacity. Frontiers in Physiology. 13, p. 859246. https://doi.org/10.3389/fphys.2022.859246
Direct identification of A-to-I editing sites with nanopore native RNA sequencing
Nguyen, Tram, Heng, Jia, Kaewsapsak, Pornchai, Kok, Eng, Stanojevic, Dominik, Liu, Hao, Cardilla, Angelysia, Praditya, Albert, Yi, Zirong, Lin, Mingwan, Aw, Jong, Ho, Yin, Peh, Kai, Wang, Yuanming, Zhong, Qixing, Heraud-Farlow, Jacki, Xue, Shifeng, Reversade, Bruno, Walkley, Carl Richard, ... Tan, Meng. (2022). Direct identification of A-to-I editing sites with nanopore native RNA sequencing. Nature Methods. 19, pp. 833-844. https://doi.org/10.1038/s41592-022-01513-3
Blocking AMPK signalling to acetyl-CoA carboxylase increases cisplatin-induced acute kidney injury and suppresses the benefit of metformin
Harley, Geoff, Katerelos, Marina, Gleich, Kurt, de Souza, David P., Narayana, Vinod K., Kemp, Bruce E., Power, David A. and Mount, Peter F.. (2022). Blocking AMPK signalling to acetyl-CoA carboxylase increases cisplatin-induced acute kidney injury and suppresses the benefit of metformin. Biomedicine and Pharmacotherapy. 153, p. Article 113377. https://doi.org/10.1016/j.biopha.2022.113377
AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue
Chuang, Sheng-Ju, Johanns, Manuel, dit Ruys, Sébastien, Steinberg, Gregory R., Kemp, Bruce Ernest E., Viollet, Benoit and Rider, Mark. (2022). AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue. Biochemical Journal. 478(21), pp. 3869-3889. https://doi.org/10.1042/BCJ20210411
Post-translational modifications of the energy guardian AMP-activated protein kinase
Ovens, Ashley J., Scott, John W., Langendorf, Christopher G., Kemp, Bruce E., Oakhill, Jonathan S. and Smiles, William J.. (2021). Post-translational modifications of the energy guardian AMP-activated protein kinase. International Journal of Molecular Sciences. 22(3), p. Article 1229. https://doi.org/10.3390/ijms22031229
Cellular bioenergetics and AMPK and TORC1 signalling in blood lymphoblasts are biomarkers of clinical status in FMR1 premutation carriers
Loesch, Danuta Z., Kemp, Bruce E., Bui, Minh Q., Fisher, Paul R., Allan, Claire Y., Sanislav, Oana, Ngoel, Kevin R. W., Atkinson, Anna, Tassone, Flora, Annesley, Sarah J. and Storey, Elsdon. (2021). Cellular bioenergetics and AMPK and TORC1 signalling in blood lymphoblasts are biomarkers of clinical status in FMR1 premutation carriers. Frontiers in Psychiatry. 12, p. Article 747268. https://doi.org/10.3389/fpsyt.2021.747268
Relationships between mitochondrial function, AMPK, and TORC1 signaling in lymphoblasts with premutation alleles of the FMR1 gene
Fisher, Paul R., Allan, Claire Y., Sanislav, Oana, Atkinson, Anna, Ngoei, Kevin R. W., Kemp, Bruce E., Storey, Elsdon, Loesch, Danuta Z. and Annesley, Sarah J.. (2021). Relationships between mitochondrial function, AMPK, and TORC1 signaling in lymphoblasts with premutation alleles of the FMR1 gene. International Journal of Molecular Sciences. 22(19), p. Article 10393. https://doi.org/10.3390/ijms221910393
Rothmund-Thomson syndrome-like RECQL4 truncating mutations cause a haploinsufficient low-bone-mass phenotype in mice
Castillo-Tandazo, Wilson, Frazier, Ann E., Sims, Natalie A., Smeets, Monique F. and Walkley, Carl R.. (2021). Rothmund-Thomson syndrome-like RECQL4 truncating mutations cause a haploinsufficient low-bone-mass phenotype in mice. Molecular and Cellular Biology. 41(3), pp. Article e00590-20. https://doi.org/10.1128/MCB.00590-20
Mice with whole-body disruption of AMPK-glycogen binding have increased adiposity, reduced fat oxidation and altered tissue glycogen dynamics
Janzen, Natalie, Whitfield, Jamie, Murray-Segal, Lisa, Kemp, Bruce E., Hawley, John A. and Hoffman, Nolan J.. (2021). Mice with whole-body disruption of AMPK-glycogen binding have increased adiposity, reduced fat oxidation and altered tissue glycogen dynamics. International Journal of Molecular Sciences. 22(17), p. Article: 9616. https://doi.org/10.3390/ijms22179616
Salsalate reduces atherosclerosis through AMPKβ1 in mice
Day, Emily A., Ford, Rebecca J., Smith, Brennan K., Houde, Vanessa P., Stypa, Stephanie, Rehal, Sonia, Lhotak, Sarka, Kemp, Bruce E., Trigatti, Bernardo L., Werstuck, Geoff H., Austin, Richard C., Fullerton, Morgan D. and Steinberg, Gregory R.. (2021). Salsalate reduces atherosclerosis through AMPKβ1 in mice. Molecular Metabolism. 53, p. Article: 101321. https://doi.org/10.1016/j.molmet.2021.101321
Voluntary physical activity protects against olanzapine-induced hyperglycemia
Shamshoum, Hesham, McKie, Greg L., Medak, Kyle D., Ashworth, Kristen E., Kemp, Bruce E. and Wright, David C.. (2021). Voluntary physical activity protects against olanzapine-induced hyperglycemia. Journal of Applied Physiology. 130(2), pp. 466-478. https://doi.org/10.1152/japplphysiol.00876.2020
AMPK mediates energetic stress-induced liver GDF15
Townsend, Logan K., Weber, Alyssa J., Day, Emily A., Shamshoum, Hesham, Shaw, Simon J., Perry, Christopher G. R., Kemp, Bruce E., Steinberg, Gregory R. and Wright, David C.. (2020). AMPK mediates energetic stress-induced liver GDF15. The FASEB Journal. 35(1), p. e21218. https://doi.org/10.1096/fj.202000954R
Foam cell induction activates ampk but uncouples its regulation of autophagy and lysosomal homeostasis
LeBlond, Nicholas D., Nunes, Julia R. C., Smith, Tyler K. T., O’Dwyer, Conor, Robichaud, Sabrina, Gadde, Suresh, Côté, Marceline, Kemp, Bruce E., Ouimet, Mireille and Fullerton, Morgan D.. (2020). Foam cell induction activates ampk but uncouples its regulation of autophagy and lysosomal homeostasis. International Journal of Molecular Sciences. 21(23), pp. 1-21. https://doi.org/10.3390/ijms21239033
Functional analysis of an R311C variant of Ca2+-calmodulin-dependent protein kinase kinase-2 (CaMKK2) found as a de novo mutation in a patient with bipolar disorder
Xy Ling, Naomi, Langendorf, Christopher G., Hoque, Ashfaqul, Galic, Sandra, Loh, Kim, Kemp, Bruce E., Gundlach, Andrew L., Oakhill, Jonathan S. and Scott, John W.. (2020). Functional analysis of an R311C variant of Ca2+-calmodulin-dependent protein kinase kinase-2 (CaMKK2) found as a de novo mutation in a patient with bipolar disorder. Bipolar Disorders. 22(8), pp. 841-848. https://doi.org/10.1111/bdi.12901
CaMKK2 is inactivated by cAMP-PKA signaling and 14-3-3 adaptor proteins
Langendorf, Christopher G., O'Brien, Matthew T., Ngoei, Kevin R. W., McAloon, Luke M., Dhagat, Urmi, Hoque, Ashfaqul, Ling, Naomi X. Y., Dite, Toby A., Galic, Sandra, Loh, Kim, Parker, Michael W., Oakhill, Jonathan S., Kemp, Bruce E. and Scott, John W.. (2020). CaMKK2 is inactivated by cAMP-PKA signaling and 14-3-3 adaptor proteins. Journal of Biological Chemistry. 295(48), pp. 16239-16250. https://doi.org/10.1074/jbc.RA120.013756
What do editors do? Understanding the physiological functions of A-to-I RNA editing by adenosine deaminase acting on RNAs
Heraud-Farlow, Jacki E. and Walkley, Carl R.. (2020). What do editors do? Understanding the physiological functions of A-to-I RNA editing by adenosine deaminase acting on RNAs. Open Biology. 10(7), p. 200085. https://doi.org/10.1098/rsob.200085
The myokine meteorin-like (metrnl) improves glucose tolerance in both skeletal muscle cells and mice by targeting AMPKa2
Lee, Jung Ok, Byun, Won Seok, Kang, Min Ju, Han, Jeong Ah, Moon, Jiyoung, Shin, Min-Jeong, Lee, Ho Jun, Chung, Ji Hyung, Lee, Jin-Seok, Son, Chang-Gue, Song, Kwon-Ho, Kim, Tae Woo, Lee, Eun-Soo, Kim, Hong Min, Chung, Choon Hee, Ngoei, Kevin R. W., Ling, Naomi X. Y., Oakhill, Jonathan S., Galic, Sandra, ... Kim, Hyeon Soo. (2020). The myokine meteorin-like (metrnl) improves glucose tolerance in both skeletal muscle cells and mice by targeting AMPKa2. The FEBS Journal. 287(10), pp. 2087-2104. https://doi.org/10.1111/FEBS.15301
Hematopoietic stem and progenitor cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia
Vu, Therese, Straube, Jasmin, Porter, Amy H., Bywater, Megan, Song, Axia, Ling, Victoria, Cooper, Leanne, Pali, Gabor, Bruedigam, Claudia, Jacquelin, Sebastien, Green, Joanne, Magor, Graham, Perkins, Andrew, Chalk, Alistair M., Walkley, Carl R., Heidel, Florian H., Mukhopadhyay, Pamela, Cloonan, Nicole, Gröschel, Stefan, ... Lane, Steven W.. (2020). Hematopoietic stem and progenitor cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia. Nature Communications. 11, pp. 1-15. https://doi.org/10.1038/s41467-020-16840-2
Genetic loss of AMPK-glycogen binding destabilizes AMPK and disrupts metabolism
Hoffman, Nolan J., Whitfield, Jamie, Janzen, Natalie R., Belhaj, Mehdi R., Galic, Sandra, Murray-Segal, Lisa, Smiles, William J., Ling, Naomi X. Y., Dite, Toby A., Scott, John W., Oakhill, Jonathan S., Brink, Robert, Kemp, Bruce E. and Hawley, John A.. (2020). Genetic loss of AMPK-glycogen binding destabilizes AMPK and disrupts metabolism. Molecular Metabolism. 41, pp. 1-13. https://doi.org/10.1016/j.molmet.2020.101048
Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms
Pinkosky, Stephen L., Scott, John W., Desjardins, Eric M., Smith, Brennan K., Day, Emily A., Ford, Rebecca J., Langendorf, Christopher G., Ling, Naomi X. Y., Nero, Tracy L., Loh, Kim, Galic, Sandra, Hoque, Ashfaqul, Smiles, William J., Ngoei, Kevin R. W., Parker, Michael W., Yan, Yan, Melcher, Karsten, Kemp, Bruce E., Oakhill, Jonathan S. and Steinberg, Gregory R.. (2020). Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms. Nature Metabolism. 2(9), pp. 873-881. https://doi.org/10.1038/s42255-020-0245-2
Enhancing mitochondrial function in vivo rescues MDS-like anemia induced by pRb deficiency
Sen, Taha, Jain, Mayur, Gram, Magnus, Mattebo, Alexander, Soneji, Shamit, Walkley, Carl R. and Singbrant, Sofie. (2020). Enhancing mitochondrial function in vivo rescues MDS-like anemia induced by pRb deficiency. Experimental Hematology. 88, pp. 28-41. https://doi.org/10.1016/j.exphem.2020.06.006
Effects of PKB/Akt inhibitors on insulin-stimulated lipogenesis and phosphorylation state of lipogenic enzymes in white adipose tissue
Hussain, Nusrat, Chuang, Sheng-Ju, Johanns, Manuel, Vertommen, Didier, Steinberg, Gregory R., Kemp, Bruce E. and Rider, Mark H.. (2020). Effects of PKB/Akt inhibitors on insulin-stimulated lipogenesis and phosphorylation state of lipogenic enzymes in white adipose tissue. Biochemical Journal. 477(8), pp. 1373-1389. https://doi.org/10.1042/BCJ20190788
Dynamic regulation of Z-DNA in the mouse prefrontal cortex by the RNA-editing enzyme Adar1 is required for fear extinction
Marshall, Paul R., Zhao, Qiongyi, Li, Xiang, Wei, Wei, Periyakaruppiah, Ambika, Zajaczkowski, Esmi L., J. Leighton, Laura, Madugalle, Sachithrani U., Basic, Dean, Wang, Ziqi, Yin, Jiayu, Liau, Wei-Siang, Gupte, Ankita, Walkley, Carl R. and Bredy, Timothy W.. (2020). Dynamic regulation of Z-DNA in the mouse prefrontal cortex by the RNA-editing enzyme Adar1 is required for fear extinction. Nature Neuroscience. 23(6), pp. 718-729. https://doi.org/10.1038/s41593-020-0627-5
AMPK β1 activation suppresses antipsychotic-induced hyperglycemia in mice
Shamshoum, Hesham, Medak, Kyle D., Townsend, Logan K., Ashworth, Kristen E., Bush, Natasha D., Halm, Margaret K., Kemp, Bruce E. and Wright, David C.. (2019). AMPK β1 activation suppresses antipsychotic-induced hyperglycemia in mice. The FASEB Journal. 33(12), pp. 14010-14021. https://doi.org/10.1096/fj.201901820R
Murine models of bone sarcomas
Jacques, Camille, Renema, Nathalie, Ory, Benjamin, Walkley, Carl R., Grigoriadis, Agamemnon E. and Heymann, Dominique. (2019). Murine models of bone sarcomas. In In Idris, Aymen I. (Ed.). Bone research protocols pp. 331-342 Humana Press. https://doi.org/10.1007/978-1-4939-8997-3_18
Osteosarcoma in the Post Genome Era: Preclinical Models and Approaches to Identify Tractable Therapeutic Targets
Wilson Castillo-Tandazo, Anthony J. Mutsaers and Carl R. Walkley. (2019). Osteosarcoma in the Post Genome Era: Preclinical Models and Approaches to Identify Tractable Therapeutic Targets. Current Osteoporosis Reports. 17(5), pp. 343-352. https://doi.org/10.1007/s11914-019-00534-w
ATP synthase inhibitory factor 1 (IF1), a novel myokine, regulates glucose metabolism by AMPK and Akt dual pathways
Lee, Hye Jeong, Moon, Jiyoung, Chung, InHyeok, Chung, Ji Hyung, Park, Chan, Lee, Jung Ok, Han, Jeong Ah, Kang, Min Ju, Yoo, Eun Hye, Kwak, So-Young, Jo, Garam, Park, Wonil, Park, Jonghoon, Kim, Kyoung Min, Lim, Soo, Ngoei, Kevin R. W., Ling, Naomi X. Y., Oakhill, Jonathan S., Galic, Sandra, ... Kim, Hyeon Soo. (2019). ATP synthase inhibitory factor 1 (IF1), a novel myokine, regulates glucose metabolism by AMPK and Akt dual pathways. The FASEB Journal. 33(12), pp. 14825 - 14840. https://doi.org/10.1096/fj.201901440RR
Cell death following the loss of ADAR1 mediated A-to-I RNA editing is not effected by the intrinsic apoptosis pathway
Walkley, Carl R. and Kile, Benjamin T.. (2019). Cell death following the loss of ADAR1 mediated A-to-I RNA editing is not effected by the intrinsic apoptosis pathway. Cell Death and Disease. 10, pp. 1 - 5. https://doi.org/10.1038/s41419-019-2160-6
Absence of the β1 subunit of AMP-activated protein kinase reduces myofibroblast infiltration of the kidneys in early diabetes
Choy, Suet-Wan, Fraser, Scott A., Katerelos, Marina, Galic, Sandra, Kemp, Bruce E., Mount, Peter F. and Power, David A.. (2019). Absence of the β1 subunit of AMP-activated protein kinase reduces myofibroblast infiltration of the kidneys in early diabetes. International Journal of Experimental Pathology. 100(2), pp. 114 - 122. https://doi.org/10.1111/iep.12313
Defining the functions of adenosine-to-inosine RNA editing through hematology
Heraud-Farlow, Jacki E., Chalk, Alistair M. and Walkley, Carl R.. (2019). Defining the functions of adenosine-to-inosine RNA editing through hematology. Current Opinion in Hematology. 26(4), pp. 241 - 248. https://doi.org/10.1097/MOH.0000000000000514
Modeling human RNA spliceosome mutations in the mouse: Not all mice were created equal
Xu, Jane Jialu, Smeets, Monique F., Tan, Shuh Ying, Wall, Meaghan, Purton, Louise E. and Walkley, Carl R.. (2019). Modeling human RNA spliceosome mutations in the mouse: Not all mice were created equal. Experimental Hematology. 70, pp. 10 - 23. https://doi.org/10.1016/j.exphem.2018.11.001
Inhibition of adenosine monophosphate-activated protein kinase-3-hydroxy-3-methylglutaryl coenzyme a reductase signaling leads to hypercholesterolemia and promotes hepatic steatosis and insulin resistance
Loh, Kim, Tam, Shanna, Murray-Segal, Lisa, Huynh, Kevin, Meikle, Peter J., Scott, John W., van Denderen, Bryce J., Chen, Zhiping, Steel, Rohan, LeBlond, Nicholas D., Burkovsky, Leah A., O'Dwyer, Conor, Nunes, Julia R. C., Steinberg, Gregory R., Fullerton, Morgan D., Galic, Sandra and Kemp, Bruce E.. (2019). Inhibition of adenosine monophosphate-activated protein kinase-3-hydroxy-3-methylglutaryl coenzyme a reductase signaling leads to hypercholesterolemia and promotes hepatic steatosis and insulin resistance. Hepatology Communications. 3(1), pp. 84 - 98. https://doi.org/10.1002/hep4.1279
Hemopoietic Cell Kinase amplification with Protein Tyrosine Phosphatase Receptor T depletion leads to polycythemia, aberrant marrow erythoid maturation, and splenomegaly
Ku, Matthew, MacKinnon, Ruth N., Wall, Meaghan, Narayan, Nisha, Walkley, Carl, Cheng, Heung-Chin, Campbell, Lynda J., Purton, Louise E. and Nandurkar, Harshal. (2019). Hemopoietic Cell Kinase amplification with Protein Tyrosine Phosphatase Receptor T depletion leads to polycythemia, aberrant marrow erythoid maturation, and splenomegaly. Scientific Reports. 9(1), pp. 1 - 11. https://doi.org/10.1038/s41598-019-43373-6
ATP-dependent helicase activity is dispensable for the physiological functions of Recql4
Castillo-Tandazo, Wilson, Smeets, Monique F., Murphy, Vincent, Liu, Rui, Hodson, Charlotte, Heierhorst, Jörg, Deans, Andrew J. and Walkley, Carl R.. (2019). ATP-dependent helicase activity is dispensable for the physiological functions of Recql4. PLoS Genetics. 15(7), pp. 1 - 19. https://doi.org/10.1371/journal.pgen.1008266
The majority of A-to-I RNA editing is not required for mammalian homeostasis
Chalk, Alistair M., Taylor, Scott, Heraud-Farlow, Jacki E. and Walkley, Carl. (2019). The majority of A-to-I RNA editing is not required for mammalian homeostasis. Genome Biology. 20, pp. 1 - 14. https://doi.org/10.1186/s13059-019-1873-2
Smac mimetics LCL161 and GDC-0152 inhibit osteosarcoma growth and metastasis in mice
Shekhar, Tanmay M., Burvenich, Ingrid J. G., Harris, Michael A., Rigopoulos, Angela, Zanker, Damien, Spurling, Alex, Parker, Belinda S., Walkley, Carl R., Scott, Andrew M. and Hawkins, Christine J.. (2019). Smac mimetics LCL161 and GDC-0152 inhibit osteosarcoma growth and metastasis in mice. BMC Cancer. 19, pp. 1 - 18. https://doi.org/10.1186/s12885-019-6103-5
Phosphorylation of Acetyl-CoA Carboxylase by AMPK reduces renal fibrosis and is essential for the anti-fibrotic effect of metformin
Lee, Mardiana, Kateleros, Marina, Gleich, Kurt, Galic, Sandra, Kemp, Bruce, Mount, Peter F. and Power, David A.. (2018). Phosphorylation of Acetyl-CoA Carboxylase by AMPK reduces renal fibrosis and is essential for the anti-fibrotic effect of metformin. Journal of the American Society of Nephrology. 29(9), pp. 2326 - 2336. https://doi.org/10.1681/ASN.2018010050
Srsf2P95H initiates myeloid bias and myelodysplastic/myeloproliferative syndrome from hemopoietic stem cells
Smeets, Monique F., Tan, Shuh Ying, Xu, Jane Jialu, Anande, Govardhan, Unnikrishnan, Ashwin, Chalk, Alistair M., Taylor, Scott, Pimanda, John E., Wall, Meaghan, Purton, L. E. and Walkley, Carl. (2018). Srsf2P95H initiates myeloid bias and myelodysplastic/myeloproliferative syndrome from hemopoietic stem cells. Blood. 132(6), pp. 608 - 621. https://doi.org/10.1182/blood-2018-04-845602
Small animal models for the study of bone sarcoma pathogenesis: characteristics, therapeutic interests and limitations
Jacques, Camille, Renema, Nathalie, Lezot, Frederic, Ory, Benjamin, Walkley, Carl, Grigoriadis, Agi E. and Heymann, Dominique. (2018). Small animal models for the study of bone sarcoma pathogenesis: characteristics, therapeutic interests and limitations. Journal of Bone Oncology. 12, pp. 7 - 13. https://doi.org/10.1016/j.jbo.2018.02.004
Loss of BIM increases mitochondrial oxygen consumption and lipid oxidation, reduces adiposity and improves insulin sensitivity in mice
Wali, Jibran A., Galic, Sandra, Tan, Christina Y. R., Gurzov, Esteban N., Frazier, Ann E., Connor, Timothy, Ge, Jingjing, Pappas, Evan G., Stroud, David, Varanasi, L. Chitra, Selck, Claudia, Ryan, Michael T., Thorburn, David R., Kemp, Bruce, Krishnamurthy, Balasubramanian, Kay, Thomas W. H., McGee, S. L. and Thomas, Helen. (2018). Loss of BIM increases mitochondrial oxygen consumption and lipid oxidation, reduces adiposity and improves insulin sensitivity in mice. Cell Death and Differentiation. 25(1), pp. 217 - 225. https://doi.org/10.1038/cdd.2017.168
AMPK signaling to acetyl-CoA carboxylase is required for fasting - and cold-induced appetite but not thermogenesis
Galic, Sandra, Loh, Kim, Murray-Segal, Lisa, Steinberg, Gregory R., Andrews, Zane B. and Kemp, Bruce. (2018). AMPK signaling to acetyl-CoA carboxylase is required for fasting - and cold-induced appetite but not thermogenesis. eLife. 7, pp. 1 - 22. https://doi.org/10.7554/eLife.32656
Structural determinants for small-molecule activation of skeletal muscle AMPK α2β2γ1 by the glucose importagog sc4
Ngoei, Kevin R.W., Langendorf, Christopher G., Ling, Naomi, Hoque, Ashfaqul, Johnson, Swapna, Camerino, Michelle C., Walker, Scott R., Bozikis, Ylva E., Dite, Toby A., Ovens, Ashley J., Smiles, William, Jacobs, Roxane, Huang, He, Parker, Michael W., Scott, John W., Rider, Mark H., Foitzik, Richard C., Kemp, Bruce, Baell, Jonathan B. and Oakhill, Jonathan S.. (2018). Structural determinants for small-molecule activation of skeletal muscle AMPK α2β2γ1 by the glucose importagog sc4. Cell Chemical Biology. 25(6), pp. 728 - 737. https://doi.org/10.1016/j.chembiol.2018.03.008
AMPK-ACC signaling modulates platelet phospholipids and potentiates thrombus formation
Lepropre, Sophie, Kautbally, Shakeel, Octave, Marie, Ginion, Audrey, Onselaer, Marie-Blanche, Steinberg, Gregory R., Kemp, Bruce E., Hego, Alexandre, Wéra, Odile, Brouns, Sanne, Swieringa, Frauke, Giera, Martin, Darley-Usmar, Victor M., Ambroise, Jérôme, Guigas, Bruno, Heemskerk, Johan, Bertrand, Luc, Oury, Cécile, Beauloye, Christophe and Horman, Sandrine. (2018). AMPK-ACC signaling modulates platelet phospholipids and potentiates thrombus formation. Blood. 132(11), pp. 1180 - 1192. https://doi.org/10.1182/blood-2018-02-831503
The spectrum of neurological and white matter changes and premutation status categories of older male carriers of the FMR1 alleles are linked to genetic (CGG and FMR1 mRNA) and cellular stress (AMPK) markers
Loesch, Danuta Z., Trost, Nicholas, Bui, Minh Q., Hammersley, Eleanor, Lay, Sui T., Annesley, Sarah J., Sanislav, Oana, Allan, Claire Y., Tassone, Flora, Chen, Zhi-Ping, Ngoei, Kevin R. W., Kemp, Bruce E., Francis, David, Fisher, Paul R. and Storey, Elsdon. (2018). The spectrum of neurological and white matter changes and premutation status categories of older male carriers of the FMR1 alleles are linked to genetic (CGG and FMR1 mRNA) and cellular stress (AMPK) markers. Frontiers in Genetics. 9, pp. 1 - 12. https://doi.org/10.3389/fgene.2018.00531
Visualizing AMPK drug binding sites through crystallization of full-length phosphorylated α2β1γ1 heterotrimer
Langendorf, Christopher G., Oakhill, Jonathan S. and Kemp, Bruce E.. (2018). Visualizing AMPK drug binding sites through crystallization of full-length phosphorylated α2β1γ1 heterotrimer. In In D. Neumann and B. Viollet (Ed.). AMPK: Methods and protocol pp. 15 - 27 Humana Press, Inc.. https://doi.org/10.1007/978-1-4939-7598-3_2
ADAR1-mediated RNA editing is required for thymic self-tolerance and inhibition of autoimmunity
Nakahama, Taisuke, Kato, Yuki, Kim, Jung In, Vongpipatana, Tuangtong, Suzuki, Yutaka, Walkley, Carl R. and Kawahara, Yukio. (2018). ADAR1-mediated RNA editing is required for thymic self-tolerance and inhibition of autoimmunity. EMBO Reports. 19(12), pp. 1 - 18. https://doi.org/10.15252/embr.201846303
Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells
Hoque, Ashfaqul, Sivakumaran, Priyadharshini, Bond, Simon T., Ling, Naomi, Kong, Anne M., Scott, John W., Bandara, Nadeeka, Hernandez, Damian, Liu, Guei-Sheung, Wong, Raymond, Ryan, Michael T., Hausenloy, Derek, Kemp, Bruce, Oakhill, Jonathan S., Drew, Brian, Pebay, Alice and Lim, Shiang Y.. (2018). Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells. Cell Death Discovery. 4(39), pp. 1 - 13. https://doi.org/10.1038/s41420-018-0042-9
mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis
Martin, Sally K., Fitter, Stephen, El Khawanky, Nadia, Grose, Randall H., Walkley, Carl, Purton, Louise E., Ruegg, Markus A., Hall, Michael N., Gronthos, Stan and Zannettino, Andrew C. W.. (2018). mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis. Scientific Reports. 8(1), pp. 1 - 10. https://doi.org/10.1038/s41598-018-32858-5
Adar3 is involved in learning and memory in mice
Mladenova, Dessislava, Barry, Guy, Konen, Lyndsey M., Pineda, Sandy S., Guennewig, Boris, Avesson, Lotta, Zinn, Raphael, Schonrock, Nicole, Bitar, Maina, Jonkhout, Nicky, Crumlish, Lauren, Kaczorowski, Dominik C., Gong, Andrew, Pinese, Mark, Franco, Gloria R., Walkley, Carl R., Vissel, Bryce and Mattick, John S.. (2018). Adar3 is involved in learning and memory in mice. Frontiers in Neuroscience. 12, pp. 1 - 17. https://doi.org/10.3389/fnins.2018.00243
AMP-activated protein kinase selectively inhibited by the type II inhibitor SBI-0206965
Dite, Toby A., Langendorf, Christopher, Hoque, Ashfaqul, Galic, Sandra, Rebello, Richard J., Ovens, Ashley J., Lindqvist, Lisa M., Ngoei, Kevin R.W., Ling, Naomi, Furic, Luc, Kemp, Bruce, Scott, John W. and Oakhill, Jonathan S.. (2018). AMP-activated protein kinase selectively inhibited by the type II inhibitor SBI-0206965. Journal of Biological Chemistry. 293(23), pp. 8874 - 8885. https://doi.org/10.1074/jbc.RA118.003547
Metformin inhibits gluconeogenesis via a redox-dependent mechanism in vivo
Madiraju, Anila K., Qiu, Yang, Perry, Rachel J., Rahimi, Yasmeen, Zhang, Xian-Man, Zhang, Dongyan, Camporez, João-Paulo G., Cline, Gary W., Butrico, Gina M., Kemp, Bruce, Casals, Gregori, Steinberg, Gregory R., Vatner, Daniel F., Petersen, Kitt F. and Shulman, Gerald I.. (2018). Metformin inhibits gluconeogenesis via a redox-dependent mechanism in vivo. Nature Medicine. 24(9), pp. 1384-1394. https://doi.org/10.1038/s41591-018-0125-4
Tolerance to sustained activation of the cAMP/Creb pathway activity in osteoblastic cells is enabled by loss of p53
Walia, Mannu K., Taylor, Scott, Ho, Patricia M.W., Martin, John and Walkley, Carl. (2018). Tolerance to sustained activation of the cAMP/Creb pathway activity in osteoblastic cells is enabled by loss of p53. Cell Death and Disease. 9(9), pp. 1 - 17. https://doi.org/10.1038/s41419-018-0944-8
The asymmetric cell division regulators par3, scribble and PINS/GPSM2 are not essential for erythroid development or enucleation
Wölwer, Christina B., Gödde, Nathan, Pase, Luke B., Elsum, Imogen A., Lim, Krystle Y. B., Sacirbegovic, Faruk, Walkley, Carl, Ellis, Sharon, Ohno, Shigeo, Matsuzaki, Fumio, Russell, Sarah M. and Humbert, Patrick O.. (2017). The asymmetric cell division regulators par3, scribble and PINS/GPSM2 are not essential for erythroid development or enucleation. PLoS ONE. 12(1), pp. 1 - 14. https://doi.org/10.1371/journal.pone.0170295
mTORC1 plays an important role in skeletal development by controlling preosteoblast differentiation
Fitter, Stephen, Mathews, Mary P., Martin, Sally K., Xie, Jianling, Ooi, Soo Siang, Walkley, Carl, Codrington, John D., Ruegg, Markus A., Hall, Michael N., Proud, Christopher G., Gronthos, Stan and Zannettino, Andrew C. W.. (2017). mTORC1 plays an important role in skeletal development by controlling preosteoblast differentiation. Molecular and Cellular Biology. 37(7), pp. 1 - 20. https://doi.org/10.1128/MCB.00668-16
Murine models of osteosarcoma: A piece of the translational puzzle
Walia, Mannu K., Castillo-Tandazo, Wilson, Mutsaers, Anthony J., Martin, T. John and Walkley, Carl. (2017). Murine models of osteosarcoma: A piece of the translational puzzle. Journal of Cellular Biochemistry. 119(6), pp. 4241 - 4250. https://doi.org/10.1002/jcb.26601
Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress
Shi, Wei, Vu, Therese, Boucher, Didier, Biernacka, Anna, Nde, Jules, Pandita, Raj K., Straube, Jasmin, Boyle, Glen M., Al-Ejeh, Fares, Nag, Purba, Jeffery, Jessie, Harris, Janelle L., Bain, Amanda L., Grzelak, Marta, Skrzypczak, Magdalena, Mitra, Abhishek, Dojer, Norbert, Crosetto, Nicola, Cloonan, Nicole, ... Khanna, Kum Kum. (2017). Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress. Blood. 129(18), pp. 2479 - 2492. https://doi.org/10.1182/blood-2016-06-725093
Impact of genetic variation on human CaMKK2 regulation by Ca2+ -calmodulin and multisite phosphorylation
O'Brien, Matthew, Oakhill, Jonathan S., Ling, Naomi, Langendorf, Christopher G., Hoque, Ashfaqul, Dite, Toby A., Means, Anthony R., Kemp, Bruce and Scott, John W.. (2017). Impact of genetic variation on human CaMKK2 regulation by Ca2+ -calmodulin and multisite phosphorylation. Scientific Reports. 7, pp. 1 - 11. https://doi.org/10.1038/srep43264
AMPK ß1 reduces tumor progression and improves survival in p53 null mice
Houde, Vanessa P., Donzelli, Sara, Sacconi, Andrea, Galic, Sandra, Hammill, Joanne A., Bramson, Jonathan L., Foster, Robert A., Tsakiridis, Theodoros, Kemp, Bruce, Grasso, Giuseppe, Blandino, Giovanni, Muti, Paola and Steinberg, Gregory R.. (2017). AMPK ß1 reduces tumor progression and improves survival in p53 null mice. Molecular Oncology. 11(9), pp. 1143 - 1155. https://doi.org/10.1002/1878-0261.12079
The autophagy initiator ULK1 sensitizes AMPK to allosteric drugs
Dite, Toby A., Ling, Naomi, Scott, John W., Hoque, Ashfaqul, Galic, Sandra, Parker, Benjamin L., Ngoei, Kevin R.W., Langendorf, Christopher G., O'Brien, Matthew, Kundu, Mondira, Viollet, Benoit, Steinberg, Gregory R., Sakamoto, Kei, Kemp, Bruce and Oakhill, Jonathan S.. (2017). The autophagy initiator ULK1 sensitizes AMPK to allosteric drugs. Nature Communications. 8(571), pp. 1 - 14. https://doi.org/10.1038/s41467-017-00628-y
Metabolism: Energy sensing through a sugar diphosphate
Kemp, Bruce and Oakhill, Jonathan S.. (2017). Metabolism: Energy sensing through a sugar diphosphate. Nature. 548(7665), pp. 36 - 37. https://doi.org/10.1038/nature23099
Fake inhibitors: AMPK activation trumps inhibition
Langendorf, Christopher G., Scott, John W. and Kemp, Bruce E.. (2017). Fake inhibitors: AMPK activation trumps inhibition. Cell Chemical Biology. 24(7), pp. 775 - 777. https://doi.org/10.1016/j.chembiol.2017.07.005
Dynamic landscape and regulation of RNA editing in mammals
Tan, Meng How, Li, Qin, Shanmugam, Raghuvaran, Piskol, Robert, Kohler, Jennefer, Young, Amy N., Liu, Kaiwen Ivy, Zhang, Rui, Ramaswami, Gokul, Ariyoshi, Kentaro, Gupte, Ankita, Keegan, Liam P., George, Cyril X., Ramu, Avinash, Huang, Ni, Pollina, Elizabeth A., Leeman, Dena S., Rustighi, Alessandra, Sharon Goh, Y. P., ... GTEx Consortium. (2017). Dynamic landscape and regulation of RNA editing in mammals. Nature. 550, pp. 249 - 254. https://doi.org/10.1038/nature24041
Design, synthesis, and biological activity of 1,2,3-triazolobenzodiazepine BET bromodomain inhibitors
Sharp, Phillip P., Garnier, Jean-Marc, Hatfaludi, Tamas, Xu, Zhen, Segal, David, Jarman, Kate E., Jousset, Hélène, Garnham, Alexandra, Feutrill, John T., Cuzzupe, Anthony, Hall, Peter, Taylor, Scott, Walkley, Carl, Tyler, Dean, Dawson, Mark A., Czabotar, Peter, Wilks, Andrew F., Glaser, Stefan, Huang, David C. S. and Burns, Christopher J.. (2017). Design, synthesis, and biological activity of 1,2,3-triazolobenzodiazepine BET bromodomain inhibitors. ACS Medicinal Chemistry Letters. 8(12), pp. 1298-1303. https://doi.org/10.1021/acsmedchemlett.7b00389
Protein recoding by ADAR1-mediated RNA editing is not essential for normal development and homeostasis
Heraud-Farlow, Jacki E., Chalk, Alistair M., Linder, Sandra, Li, Qin, Taylor, Scott, White, Joshua M., Pang, Lokman, Liddicoat, Brian J., Gupte, Ankita, Li, Jin Billy and Walkley, Carl. (2017). Protein recoding by ADAR1-mediated RNA editing is not essential for normal development and homeostasis. Genome Biology. 18(1), pp. 1 - 18. https://doi.org/10.1186/s13059-017-1301-4
Rewriting the transcriptome: adenosine-to-inosine RNA editing by ADARs
Walkley, Carl and Li, Jin Billy. (2017). Rewriting the transcriptome: adenosine-to-inosine RNA editing by ADARs. Genome Biology. 18(1), pp. 1 - 13. https://doi.org/10.1186/s13059-017-1347-3
ADAR1, inosine and the immune sensing system: Distinguishing self from non-self
Liddicoat, Brian J., Chalk, Alistair M. and Walkley, Carl R.. (2016). ADAR1, inosine and the immune sensing system: Distinguishing self from non-self. Wiley Interdisciplinary Reviews: RNA. 7(2), pp. 157 - 172. https://doi.org/10.1002/wrna.1322
PDGF-AB and 5-Azacytidine induce conversion of somatic cells into tissue-regenerative multipotent stem cells
Chandrakanthan, Vashe, Yeola, Avani, Kwan, Jair C., Oliver, Rema A., Qiao, Qiao, Kang, Young Chan, Zarzour, Peter, Beck, Dominik, Boelen, Lies, Unnikrishnan, Ashwin, Villanueva, Jeanette E., Nunez, Andrea C., Knezevic, Kathy, Palu, Cintia, Nasrallah, Rabab, Carnell, Michael, Macmillan, Alex, Whan, Renee, Yu, Yan, ... Pimanda, John E.. (2016). PDGF-AB and 5-Azacytidine induce conversion of somatic cells into tissue-regenerative multipotent stem cells. Proceedings of the National Academy of Sciences of USA. 113(16), pp. E2306 - E2315. https://doi.org/10.1073/pnas.1518244113
Metformin prevents nigrostriatal dopamine degeneration independent of AMPK activation in dopamine neurons
Bayliss, Jacqueline A., Lemus, Moyra B., Santos, Vanessa, Deo, Minh, Davies, Jeffrey S., Kemp, Bruce Ernest, Elsworth, John D. and Andrews, Zane B.. (2016). Metformin prevents nigrostriatal dopamine degeneration independent of AMPK activation in dopamine neurons. PLoS ONE. 11(7), pp. 1 - 15. https://doi.org/10.1371/journal.pone.0159381
Structural basis of allosteric and synergistic activation of AMPK by furan-2-phosphonic derivative C2 binding
Langendorf, Christopher G., Ngoei, Kevin R. W., Scott, John W., Ling, Naomi X. Y., Issa, Sam M. A., Gorman, Michael A., Parker, Michael W., Sakamoto, Kei, Oakhill, Jonathan S. and Kemp, Bruce Ernest. (2016). Structural basis of allosteric and synergistic activation of AMPK by furan-2-phosphonic derivative C2 binding. Nature Communications. 7, pp. 1 - 8. https://doi.org/10.1038/ncomms10912
Immortalized Parkinson's disease lymphocytes have enhanced mitochondrial respiratory activity
Annesley, Sarah J., Lay, Sui T., De Piazza, Shawn W., Sanislav, Oana, Hammersley, Eleanor, Allan, Claire Y., Francione, Lisa M., Bui, Minh Q., Chen, Zhi-Ping, Ngoei, Kevin R. W., Tassone, Flora, Kemp, Bruce E., Storey, Elsdon, Evans, Andrew, Loesch, Danuta Z. and Fisher, Paul R.. (2016). Immortalized Parkinson's disease lymphocytes have enhanced mitochondrial respiratory activity. Disease Models and Mechanisms. 9(9), pp. 1295 - 1305. https://doi.org/10.1242/dmm.025684
Defining the minimal factors required for erythropoiesis through direct lineage conversion
Capellera-Garcia, Sandra, Pulecio, Julian, Dhulipala, Kishori, Siva, Kavitha, Rayon-Estrada, Violeta, Singbrant, Sofie, Sommarin, Mikael N. E., Walkley, Carl R., Soneji, Shamit, Karlsson, Göran, Raya, Ángel, Sankaran, Vijay G. and Flygare, Johan. (2016). Defining the minimal factors required for erythropoiesis through direct lineage conversion. Cell Reports. 15(11), pp. 2550 - 2562. https://doi.org/10.1016/j.celrep.2016.05.027
Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis
Liddicoat, Brian J., Hartner, Jochen C., Piskol, Robert, Ramaswami, Gokul, Chalk, Alistair M., Kingsley, Paul D., Sankaran, Vijay G., Wall, Meaghan, Purton, Louise E., Seeburg, Peter H., Palis, James, Orkin, Stuart H., Lu, Jun, Li, Jin Billy and Walkley, Carl R.. (2016). Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis. Experimental Hematology. 44(10), pp. 947 - 963. https://doi.org/10.1016/j.exphem.2016.06.250
Ghrelin-AMPK signaling mediates the neuroprotective effects of calorie restriction in Parkinson's Disease
Bayliss, Jacqueline A., Lemus, Moyra B., Stark, Romana, Santos, Vanessa V., Thompson, Aiysha, Rees, Daniel J., Galic, Sandra, Elsworth, John D., Kemp, Bruce Ernest, Davies, Jeffrey S. and Andrews, Zane B.. (2016). Ghrelin-AMPK signaling mediates the neuroprotective effects of calorie restriction in Parkinson's Disease. Journal of Neuroscience. 36(10), pp. 3049 - 3063. https://doi.org/10.1523/JNEUROSCI.4373-15.2016
Loss of ephrinB1 in osteogenic progenitor cells impedes endochondral ossification and compromises bone strength integrity during skeletal development
Nguyen, Thao M., Arthur, Agnieszka, Paton, Sharon, Hemming, Sarah, Panagopoulos, Romana, Codrington, John D., Walkley, Carl R., Zannettino, Andrew C. W. and Gronthos, Stan. (2016). Loss of ephrinB1 in osteogenic progenitor cells impedes endochondral ossification and compromises bone strength integrity during skeletal development. Bone. 93, pp. 12 - 21. https://doi.org/10.1016/j.bone.2016.09.009
Increased miR-155-5p and reduced miR-148a-3p contribute to the suppression of osteosarcoma cell death
Bhattacharya, S., Chalk, Alistair M., Ng, A. J. M., Martin, T. John, Zannettino, Andrew C. W., Purton, L. E., Lu, J., Baker, E. K. and Walkley, Carl R.. (2016). Increased miR-155-5p and reduced miR-148a-3p contribute to the suppression of osteosarcoma cell death. Oncogene. 35(40), pp. 5282 - 5294. https://doi.org/10.1038/onc.2016.68
The transcription factor ASCIZ and its target DYNLL1 are essential for the development and expansion of MYC-driven B cell lymphoma
Wong, David M., Li, ngli Li, Jurado, Sabine, King, Ashleigh, Bamford, Rebecca, Wall, Meaghan, Walia, Mannu K., Kelly, Gemma L., Walkley, Carl R., Tarlinton, David M., Strasser, Andreas and Heierhorst, Jörg. (2016). The transcription factor ASCIZ and its target DYNLL1 are essential for the development and expansion of MYC-driven B cell lymphoma. Cell Reports. 14(6), pp. 1488 - 1499. https://doi.org/10.1016/j.celrep.2016.01.012
The role of RNA editing by ADAR1 in prevention of innate immune sensing of self-RNA
Heraud-Farlow, Jacki E. and Walkley, Carl R.. (2016). The role of RNA editing by ADAR1 in prevention of innate immune sensing of self-RNA. Journal of Molecular Medicine. 94(10), pp. 1095 - 1102. https://doi.org/10.1007/s00109-016-1416-1
Activation of PTHrP-cAMP-CREB1 signaling following p53 loss is essential for osteosarcoma initiation and maintenance
Walia, Mannu K., Ho, Patricia M. W., Taylor, Scott, Ng, Alvin J. M., Gupte, Ankita, Chalk, Alistair M., Zannettino, Andrew C. W., Martin, T. John and Walkley, Carl R.. (2016). Activation of PTHrP-cAMP-CREB1 signaling following p53 loss is essential for osteosarcoma initiation and maintenance. eLife. 5(April), pp. 1 - 31. https://doi.org/10.7554/eLife.13446
IAP antagonists sensitize murine osteosarcoma cells to killing by TNFα
Shekhar, Tanmay M., Miles, Mark A., Gupte, Ankita, Taylor, Scott, Tascone, Brianna, Walkley, Carl R. and Hawkins, Christine J.. (2016). IAP antagonists sensitize murine osteosarcoma cells to killing by TNFα. Oncotarget. 7(23), pp. 33866 - 33886. https://doi.org/10.18632/oncotarget.8980
Systematic screening identifies dual PI3K and mTOR inhibition as a conserved therapeutic vulnerability in osteosarcoma
Gupte, Ankita, Baker, E. K., Wan, Soo-San, Stewart, Elizabeth, Loh, Amos, Shelat, Anang A., Gould, Cathryn M., Chalk, Alistair M., Taylor, Scott, Lackovic, Kurt, Karlström, Åsa, Mutsaers, Anthony J., Desai, Jayesh, Madhamshettiwar, Piyush B., Zannettino, Andrew C. W., Burns, Christopher J., Huang, David C. S., Dyer, Michael A., Simpson, Kaylene J. and Walkley, Carl. (2015). Systematic screening identifies dual PI3K and mTOR inhibition as a conserved therapeutic vulnerability in osteosarcoma. Clinical Cancer Research. 21(14), pp. 3216 - 3229. https://doi.org/10.1158/1078-0432.CCR-14-3026
Wnt inhibitory factor 1 (WIF1) is a marker of osteoblastic differentiation stage and is not silenced by DNA methylation in osteosarcoma
Baker, Emma K., Taylor, Scott, Gupte, Ankita, Chalk, Alistair M., Bhattacharya, Shreya, Green, Alanna C., Martin, T. John, Strbenac, Dario, Robinson, Mark D., Purton, Louise E. and Walkley, Carl R.. (2015). Wnt inhibitory factor 1 (WIF1) is a marker of osteoblastic differentiation stage and is not silenced by DNA methylation in osteosarcoma. Bone. 73, pp. 223 - 232. https://doi.org/10.1016/j.bone.2014.12.063
Skeletal muscle AMPK is essential for the maintenance of FNDC5 expression
Lally, James S. V., Ford, Rebecca J., Johar, Jasper, Crane, Justin D., Kemp, Bruce Ernest and Steinberg, Gregory R.. (2015). Skeletal muscle AMPK is essential for the maintenance of FNDC5 expression. Physiological Reports. 3(5), pp. 1 - 9. https://doi.org/10.14814/phy2.12343
Src family kinases and their role in hematological malignancies
Ku, Matthew, Wall, Meaghan, MacKinnon, Ruth N., Walkley, Carl R., Purton, Louise E., Tam, Constantine, Izon, David, Campbell, Lynda, Cheng, Heung-Chin and Nandurkar, Harshal. (2015). Src family kinases and their role in hematological malignancies. Leukemia and Lymphoma. 56(3), pp. 577 - 586. https://doi.org/10.3109/10428194.2014.907897
HIF-1α is required for hematopoietic stem cell mobilization and 4-prolyl hydroxylase inhibitors enhance mobilization by stabilizing HIF-1α
Forristal, C. E., Nowlan, B., Jacobsen, R. N., Barbier, V., Walkinshaw, G., Walkley, Carl, Winkler, I. G. and Levesque, J. P.. (2015). HIF-1α is required for hematopoietic stem cell mobilization and 4-prolyl hydroxylase inhibitors enhance mobilization by stabilizing HIF-1α. Leukemia. 29(6), pp. 1366 - 1378. https://doi.org/10.1038/leu.2015.8
Salicylate improves macrophage cholesterol homeostasis via activation of Ampk
Fullerton, Morgan D., Ford, Rebecca J., McGregor, Chelsea P., LeBlond, Nicholas D., Snider, Shayne A., Stypa, Stephanie A., Day, Emily A., Lhoták, Šárka, Schertzer, Jonathan D., Austin, Richard C., Kemp, Bruce Ernest and Steinberg, Gregory R.. (2015). Salicylate improves macrophage cholesterol homeostasis via activation of Ampk. Journal of Lipid Research. 56(5), pp. 1025 - 1033. https://doi.org/10.1194/jlr.M058875
The DNA Helicase Recql4 is required for normal osteoblast expansion and osteosarcoma formation
Ng, A. J. M., Walia, Mannu K., Smeets, Monique F., Mutsaers, Anthony J., Sims, Natalie A., Purton, L. E., Walsh, Nicole C., Martin, T. John and Walkley, Carl. (2015). The DNA Helicase Recql4 is required for normal osteoblast expansion and osteosarcoma formation. PLoS Genetics. 11(4), pp. 1 - 25. https://doi.org/10.1371/journal.pgen.1005160
Choreography of AMPK activation
Langerdorf, Christopher G. and Kemp, Bruce Ernest. (2015). Choreography of AMPK activation. Cell Research. 25(1), pp. 5 - 6. https://doi.org/10.1038/cr.2014.163
BET inhibitors induce apoptosis through a MYC independent mechanism and synergise with CDK inhibitors to kill osteosarcoma cells
Baker, E. K., Taylor, Scott, Gupte, Ankita, Sharp, Phillip P., Walia, Mannu K., Walsh, Nicole C., Zannettino, Andrew C. W., Chalk, Alistair M., Burns, Christopher J. and Walkley, Carl. (2015). BET inhibitors induce apoptosis through a MYC independent mechanism and synergise with CDK inhibitors to kill osteosarcoma cells. Scientific Reports. 5, pp. 1 - 14. https://doi.org/10.1038/srep10120
Skeletal muscle ACC2 S212 phosphorylation is not required for the control of fatty acid oxidation during exercise
O'Neill, Hayley M., Lally, James S., Galic, Sandra, Pulinilkunnil, Thomas, Ford, Rebecca J., Dyck, Jason R. B., Van Denderen, Bryce J,, Kemp, Bruce Ernest and Steinberg, Gregory R.. (2015). Skeletal muscle ACC2 S212 phosphorylation is not required for the control of fatty acid oxidation during exercise. Physiological Reports. 3(7), pp. 1 - 10. https://doi.org/10.14814/phy2.12444
Metformin and salicylate synergistically activate liver AMPK, inhibit lipogenesis and improve insulin sensitivity
Ford, Rebecca, Fullerton, Morgan, Pinkosky, Stephen, Day, Emily, Scott, John, Oakhill, Jonathan, Bujak, Adam, Smith, Brennan, Crane, Justin, Blumer, Regje, Marcinko, Katarina, Kemp, Bruce, Gerstein, Hertzel and Steinberg, Gregory. (2015). Metformin and salicylate synergistically activate liver AMPK, inhibit lipogenesis and improve insulin sensitivity. Biochemical Journal. 468(1), pp. 125 - 132. https://doi.org/10.1042/BJ20150125
High intensity interval training improves liver and adipose tissue insulin sensitivity
Marcinko, Katarina, Sikkema, Sarah R., Samaan, M. Constantine, Kemp, Bruce Ernest, Fullerton, Morgan D. and Steinberg, Gregory R.. (2015). High intensity interval training improves liver and adipose tissue insulin sensitivity. Molecular Metabolism. 4(12), pp. 903 - 915. https://doi.org/10.1016/j.molmet.2015.09.006
RNA editing by ADAR1 prevents MDA5 sensing of endogenous dsRNA as nonself
Liddicoat, Brian J., Piskol, Robert, Chalk, Alistair M., Ramaswami, Gokul, Higuchi, Miyoko, Hartner, Jochen C., Li, Jin Billy, Seeburg, Peter H. and Walkley, Carl. (2015). RNA editing by ADAR1 prevents MDA5 sensing of endogenous dsRNA as nonself. Science. 349(6252), pp. 1115 - 1120. https://doi.org/10.1126/science.aac7049
Inhibition of AMP-activated protein kinase at the allosteric drug-binding site promotes islet insulin release
Scott, John, Galic, Sandra, Graham, Kate, Foitzik, Richard, Ling, Naomi, Dite, Toby, Issa, Samah, Langendorf, Chris, Weng, Qing, Thomas, Helen, Kay, Thomas, Birnberg, Neal, Steinberg, Gregory, Kemp, Bruce and Oakhill, Jonathan. (2015). Inhibition of AMP-activated protein kinase at the allosteric drug-binding site promotes islet insulin release. Chemistry and Biology. 22(6), pp. 705 - 711. https://doi.org/10.1016/j.chembiol.2015.05.011
Knockdown of PTHR1 in osteosarcoma cells decreases invasion and growth and increases tumor differentiation in vivo
Ho, P. W. M., Goradia, A., Russell, M. R., Chalk, Alistair M., Milley, K. M., Baker, E. K., Danks, J. A., Slavin, John, Walia, Mannu K., Crimeen-Irwin, B., Dickins, R. A., Martin, T. John and Walkley, Carl. (2015). Knockdown of PTHR1 in osteosarcoma cells decreases invasion and growth and increases tumor differentiation in vivo. Oncogene. 34(22), pp. 2922 - 2933. https://doi.org/10.1038/onc.2014.217
AMPK deficiency in cardiac muscle results in dilated cardiomyopathy in the absence of changes in energy metabolism
Sung, Miranda M., Zordoky, Beshay N. M., Bujak, Adam L., Lally, James S. V., Fung, David, Young, Martin E., Horman, Sandrine, Miller, Edward J., Light, Peter E., Kemp, Bruce Ernest, Steinberg, Gregory R. and Dyck, Jason R. B.. (2015). AMPK deficiency in cardiac muscle results in dilated cardiomyopathy in the absence of changes in energy metabolism. Cardiovascular Research. 107(2), pp. 235 - 245. https://doi.org/10.1093/cvr/cvv166
Salicylate activates AMPK and synergizes with metformin to reduce the survival of prostate and lung cancer cells ex vivo through inhibition of de novo lipogenesis
O'Brien, Andrew J., Villani, Linda A., Broadfield, Lindsay A., Houde, Vanessa P., Galic, Sandra, Blandino, Giovanni, Kemp, Bruce Ernest, Tsakiridis, Theodoros, Muti, Paola and Steinberg, Gregory R.. (2015). Salicylate activates AMPK and synergizes with metformin to reduce the survival of prostate and lung cancer cells ex vivo through inhibition of de novo lipogenesis. Biochemical Journal. 469(2), pp. 177 - 187. https://doi.org/10.1042/Bj20150122
The AMPK activator R419 improves exercise capacity and skeletal muscle insulin sensitivity in obese mice
Marcinko, Katarina, Bujak, Adam L., Lally, James S. V., Ford, Rebecca J., Wong, Tammy H., Smith, Brennan K., Kemp, Bruce Ernest, Jenkins, Yonchu, Li, Wei, Kinsella, Todd M., Hitoshi, Yasumichi and Steinberg, Gregory R.. (2015). The AMPK activator R419 improves exercise capacity and skeletal muscle insulin sensitivity in obese mice. Molecular Metabolism. 4(9), pp. 643 - 651. https://doi.org/10.1016/j.molmet.2015.06.002
RARγ is a negative regulator of osteoclastogenesis
Green, Alanna C., Poulton, Ingrid J., Vrahnas, Christina, Häusler, Karl D., Walkley, Carl, Wu, Joy Y., Martin, T. John, Gillespie, Matthew T., Chandraratna, Roshantha A. S., Quinn, Julian M. W., Sims, Natalie A. and Purton, L. E.. (2015). RARγ is a negative regulator of osteoclastogenesis. The Journal of Steroid Biochemistry and Molecular Biology. 150, pp. 46 - 53. https://doi.org/10.1016/j.jsbmb.2015.03.005
Brief report: The differential roles of mTORC1 and mTORC2 in mesenchymal stem cell differentiation
Martin, Sally K., Fitter, Stephen, Dutta, Ankit K., Matthews, Mary P., Walkley, Carl, Hall, Michael N., Ruegg, Markus A., Gronthos, Stan and Zannettino, Andrew C. W.. (2015). Brief report: The differential roles of mTORC1 and mTORC2 in mesenchymal stem cell differentiation. Stem Cells. 33(4), pp. 1359 - 1365. https://doi.org/10.1002/stem.1931
AMPK activation of muscle autophagy prevents fasting-induced hypoglycemia and myopathy during aging
Bujak, Adam L., Crane, Justin D., Lally, James S., Ford, Rebecca J., Kang, Sally J., Rebalka, Irena A., Green, Alex E., Kemp, Bruce Ernest, Hawke, Thomas J., Schertzer, Jonathan D. and Steinberg, Gregory R.. (2015). AMPK activation of muscle autophagy prevents fasting-induced hypoglycemia and myopathy during aging. Cell Metabolism. 21(6), pp. 883 - 890. https://doi.org/10.1016/j.cmet.2015.05.016
Ciliary neurotrophic factor has intrinsic and extrinsic roles in regulating B cell differentiation and bone structure
Askmyr, Maria, White, Kirby E., Jovic, Tanja, King, Hannah A., Quach, Julie M., Maluenda, Ana C., Baker, E. K., Smeets, Monique F., Walkley, Carl and Purton, L. E.. (2015). Ciliary neurotrophic factor has intrinsic and extrinsic roles in regulating B cell differentiation and bone structure. Scientific Reports. 5, pp. 1 - 13. https://doi.org/10.1038/srep15529
Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging
Crane, Justin D., MacNeil, Lauren G., Lally, James S. V., Ford, Rebecca J., Bujak, Adam L., Brar, Ikdip K., Kemp, Bruce Ernest, Raha, Sandeep, Steinberg, Gregory Robert and Tarnopolsky, Mark A.. (2015). Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging. Aging Cell. 14(4), pp. 625 - 634. https://doi.org/10.1111/acel.12341
Autophosphorylation of CaMKK2 generates autonomous activity that is disrupted by a T85S mutation linked to anxiety and bipolar disorder
Scott, John, Park, Elizabeth, Rodriguiz, Ramona, Oakhill, Jonathan, Issa, Samah, O'Brien, Matthew, Dite, Toby, Langendorf, Christopher, Wetsel, William, Means, Anthony and Kemp, Bruce. (2015). Autophosphorylation of CaMKK2 generates autonomous activity that is disrupted by a T85S mutation linked to anxiety and bipolar disorder. Scientific Reports. 5, pp. 1 - 10. https://doi.org/10.1038/srep14436
SnRK1 from Arabidopsis thaliana is an atypical AMPK
Emanuelle, Shane, Hossain, Mohammed Iqbal, Moller, Isabel E., Pedersen, Henriette L., van de Meene, Allison M. L., Doblin, Monika S., Koay, Ann, Oakhill, Jonathan S., Scott, John W., Willats, William G. T., Kemp, Bruce Ernest, Bacic, Antony, Gooley, Paul R. and Stapleton, David I.. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant Journal. 82(2), pp. 183 - 192. https://doi.org/10.1111/tpj.12813
PTHrP, its receptor, and protein kinase A activation in osteosarcoma
Walkley, Carl, Walia, Mannu K., Ho, P.W.M. and Martin, T. J.. (2014). PTHrP, its receptor, and protein kinase A activation in osteosarcoma. Molecular & Cellular Oncology. 1(4), pp. 1 - 3. https://doi.org/10.4161/23723548.2014.965624
PPARδ Activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity
Bojic, Lazar, Telford, Dawn, Fullerton, Morgan, Ford, Rebecca, Sutherland, Brian, Edwards, Jane, Sawyez, Cynthia, Gros, Robert, Kemp, Bruce, Steinberg, Gregory and Huff, Murray. (2014). PPARδ Activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity. Journal of Lipid Research. 55(7), pp. 1254 - 1266. https://doi.org/10.1194/jlr.M046037
PPARδ activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity
Bojic, Lazar A., Telford, Dawn E., Fullerton, Morgan D., Ford, Rebecca J., Sutherland, Brian G., Edwards, Jane Y., Sawyez, Cynthia G., Gros, Robert, Kemp, Bruce Ernest, Steinberg, Gregory R. and Huff, Murray W.. (2014). PPARδ activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity. Journal of Lipid Research. 55(7), pp. 1254 - 1266. https://doi.org/10.1194/jlr.M046037
Muscle-specific AMPK ß1ß2-null mice display a myopathy due to loss of capillary density in nonpostural muscles
Thomas, Melissa, Wang, David, D'Souza, Donna, Krause, Matthew, Layne, Andrew, Criswell, David, O'Neill, Hayley, Connor, Michael, Anderson, Judy, Kemp, Bruce, Steinberg, Gregory and Hawke, Thomas. (2014). Muscle-specific AMPK ß1ß2-null mice display a myopathy due to loss of capillary density in nonpostural muscles. The FASEB Journal. 28(5), pp. 2098 - 2107. https://doi.org/10.1096/fj.13-238972
Evidence for the role of AMPK in regulating PGC-1 alpha expression and mitochondrial proteins in mouse epididymal adipose tissue
Wan, Zhongxiao, Root-Mccaig, Jared, Castellani, Laura, Kemp, Bruce, Steinberg, Gregory and Wright, David. (2014). Evidence for the role of AMPK in regulating PGC-1 alpha expression and mitochondrial proteins in mouse epididymal adipose tissue. Obesity: a resourch journal. 22(3), pp. 730 - 738. https://doi.org/10.1002/oby.20605
Gene expression profiling to define the cell intrinsic role of the SKI proto-oncogene in hematopoiesis and myeloid neoplsms
Chalk, Alistair M., Liddicoat, Brian J., Walkley, Carl and Singbrant, Sofie. (2014). Gene expression profiling to define the cell intrinsic role of the SKI proto-oncogene in hematopoiesis and myeloid neoplsms. Genomics Data. 2, pp. 189 - 191. https://doi.org/10.1016/j.gdata.2014.06.022
Activation of AMPK reduces the co-transporter activity of NKCC1
Fraser, Scott A., Davies, Matthew R. P., Katerelos, Marina, Gleich, Kurt, Choy, Suet-Wan, Steel, Rohan, Galic, Sandra, Mount, Peter F., Kemp, Bruce Ernest and Power, David A.. (2014). Activation of AMPK reduces the co-transporter activity of NKCC1. Molecular Membrane Biology. 31(2-3), pp. 95 - 102. https://doi.org/10.3109/09687688.2014.902128
Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models
Perera, Nirma, Sheean, Rebecca, Scott, John, Kemp, Bruce, Horne, Malcolm and Turner, Bradley. (2014). Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models. PLoS One (online). 9(3), pp. 1 - 11. https://doi.org/10.1371/journal.pone.0090449
Reduced skeletal muscle AMPK and mitochondrial markers do not promote age-induced insulin resistance
Bujak, Adam, Blumer, Regje, Marcinko, Katarina, Fullerton, Morgan, Kemp, Bruce and Steinberg, Gregory. (2014). Reduced skeletal muscle AMPK and mitochondrial markers do not promote age-induced insulin resistance. Journal of Applied Physiology. 117(2), pp. 171 - 179. https://doi.org/10.1152/japplphysiol.01101.2013
The SKI proto-oncogene enhances the in vivo repopulation of hematopoietic stem cells and causes myeloproliferative disease
Singbrant, Sofie, Wall, Meaghan, Moody, Jennifer, Karlsson, Göran, Chalk, Alistair M., Liddicoat, Brian J., Russell, Megan R., Walkley, Carl R. and Karlsson, Stefan. (2014). The SKI proto-oncogene enhances the in vivo repopulation of hematopoietic stem cells and causes myeloproliferative disease. Haematologica. 99(4), pp. 647 - 655. https://doi.org/10.3324/haematol.2013.093971
Compensatory regulation of HDAC5 in muscle maintains metabolic adaptive responses and metabolism in response to energetic stress
McGee, Sean, Swinton, Courtney, Morrison, Shona, Gaur, Vidhi, Campbell, Duncan, Jorgensen, Sebastian, Kemp, Bruce, Baar, Keith, Steinberg, Gregory and Hargreaves, Mark. (2014). Compensatory regulation of HDAC5 in muscle maintains metabolic adaptive responses and metabolism in response to energetic stress. The FASEB Journal. 28(8), pp. 3384 - 3395. https://doi.org/10.1096/fj.14-249359
The Rothmund-Thomson syndrome helicase RECQL4 is essential for hematopoiesis
Smeets, Monique F., DeLuca, Elisabetta, Wall, Meaghan, Quach, Julie M., Chalk, Alistair M., Deans, Andrew J., Heierhorst, Jörg, Purton, Louise E., Izon, David J. and Walkley, Carl R.. (2014). The Rothmund-Thomson syndrome helicase RECQL4 is essential for hematopoiesis. Journal of Clinical Investigation. 124(8), pp. 3551 - 3565. https://doi.org/10.1172/JCI75334
ATP sensitive bi-quinoline activator of the AMP-activated protein kinase
Scott, John, Oakhill, Jonathan, Ling, Naomi, Langendorf, Christopher, Foitzik, Richard, Kemp, Bruce and Issinger, Olaf-Georg. (2014). ATP sensitive bi-quinoline activator of the AMP-activated protein kinase. Biochemical and Biophysical Research Communications. 443(2), pp. 435 - 440. https://doi.org/10.1016/j.bbrc.2013.11.130
Enhanced activation of cellular AMPK by dual-small molecule treatment: AICAR and A769662
Ducommun, Serge, Ford, Rebecca J., Bultot, Laurent, Deak, Maria, Bertrand, Luc, Kemp, Bruce Ernest, Steinberg, Gregory R. and Sakamoto, Kei. (2014). Enhanced activation of cellular AMPK by dual-small molecule treatment: AICAR and A769662. American Journal of Physiology - Endocrinology and Metabolism. 306(6), pp. 688 - 696. https://doi.org/10.1152/ajpendo.00672.2013
Small molecule drug A-769662 and AMP synergistically activate naive AMPK independent of upstream kinase signaling
Scott, John, Ling, Naomi, Issa, Samah, Dite, Toby, O'Brien, Matthew, Chen, Zhi-Ping, Galic, Sandra, Langendorf, Christopher, Steinberg, Gregory, Kemp, Bruce and Oakhill, Jonathan. (2014). Small molecule drug A-769662 and AMP synergistically activate naive AMPK independent of upstream kinase signaling. Chemistry and Biology. 21(5), pp. 619 - 627. https://doi.org/10.1016/j.chembiol.2014.03.006
Novel mechanisms of Na+ retention in obesity: Phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK
Davies, Matthew R. P., Fraser, Scott A., Galic, Sandra, Choy, Suet-Wan, Katerelos, Marina, Gleich, Kurt, Kemp, Bruce Ernest, Mount, Peter F. and Power, David A.. (2014). Novel mechanisms of Na+ retention in obesity: Phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK. American Journal of Physiology - Renal Physiology. 307(1), pp. 96 - 106. https://doi.org/10.1152/ajprenal.00524.2013
Mechanism of action of compound-13 : An a1-selective small molecule activator of AMPK
Hunter, Roger, Foretz, Marc, Bultot, Laurent, Fullerton, Morgan, Deak, Maria, Ross, Fiona, Hawley, Simon, Shpiro, Natalia, Viollet, Benoit, Barron, Denis, Kemp, Bruce, Steinberg, Gregory, Hardie, David Grahame and Sakamoto, Kei. (2014). Mechanism of action of compound-13 : An a1-selective small molecule activator of AMPK. Chemistry and Biology. 21(7), pp. 866 - 879. https://doi.org/10.1016/j.chembiol.2014.05.014
Cells of origin in osteosarcoma: Mesenchymal stem cells or osteoblast committed cells?
Mutsaers, Anthony J. and Walkley, Carl R.. (2014). Cells of origin in osteosarcoma: Mesenchymal stem cells or osteoblast committed cells? Bone. 62, pp. 56 - 63. https://doi.org/10.1016/j.bone.2014.02.003
Immune response to rb1-regulated senescence limits radiation-Induced osteosarcoma formation
Kansara, Maya, Leong, Huei San, Lin, Dan Mei, Popkiss, Sophie, Pang, Puiyi, Garsed, Dale W., Walkley, Carl R., Cullinane, Carleen, Ellul, Jason, Haynes, Nicole M., Hicks, Rod, Kuijjer, Marieke L., Cleton-Jansen, Anne-Marie, Hinds, Philip W., Smyth, Mark J. and Thomas, David M.. (2013). Immune response to rb1-regulated senescence limits radiation-Induced osteosarcoma formation. Journal of Clinical Investigation. 123(12), pp. 5351 - 5360. https://doi.org/10.1172/JCI70559
Erythroid-extrinsic regulation of normal erythropoiesis by retinoic acid receptors
Dewamitta, Sita R., Joseph, Chacko, Purton, Louise E. and Walkley, Carl R.. (2013). Erythroid-extrinsic regulation of normal erythropoiesis by retinoic acid receptors. British Journal of Haematology. 164(2), pp. 280 - 285. https://doi.org/10.1111/bjh.12578
Modeling distinct osteosarcoma subtypes in vivo using Cre: Lox and lineage-restricted transgenic shRNA
Mutsaers, Anthony J., Ng, Alvin J. M., Baker, Emma K., Russell, Megan R., Chalk, Alistair M., Wall, Meaghan, Liddicoat, Brian J. J., Ho, Patricia W. M., Slavin, John L., Goradia, Ankita, Martin, T. John, Purton, Louise E., Dickins, Ross A. and Walkley, Carl R.. (2013). Modeling distinct osteosarcoma subtypes in vivo using Cre: Lox and lineage-restricted transgenic shRNA. Bone. 55(1), pp. 166 - 178. https://doi.org/10.1016/j.bone.2013.02.016
Darbepoietin-alfa has comparable erythropoietic stimulatory effects to recombinant erythropoietin whilst preserving the bone marrow microenvironment
Dewamitta, Sita R., Russell, Megan R., Nandurkar, Harshal and Walkley, Carl R.. (2013). Darbepoietin-alfa has comparable erythropoietic stimulatory effects to recombinant erythropoietin whilst preserving the bone marrow microenvironment. Haematologica. 98(5), pp. 686 - 690. https://doi.org/10.3324/haematol.2012.078709
Single phosphorylation sites in Acc1 and Acc2 regulate lipid homeostasis and the insulin-sensitizing effects of metformin
Fullerton, Morgan, Galic, Sandra, Marcinko, Katarina, Sikkema, Sarah, Pulinilkunnil, Thomas, Chen, Zhi-Ping, O'Neill, Hayley, Ford, Rebecca, Palanivel, Rengasamy, O'Brien, Matthew, Hardie, D. Grahame, Macaulay, Lance, Schertzer, Jonathan, Dyck, Jason, van Denderen, Bryce, Kemp, Bruce and Steinberg, Gregory. (2013). Single phosphorylation sites in Acc1 and Acc2 regulate lipid homeostasis and the insulin-sensitizing effects of metformin. Nature Medicine. 19(12), pp. 1649 - 1654. https://doi.org/10.1038/nm.3372
Deciphering hematopoietic stem cells in their niches: A critical appraisal of genetic models, lineage tracing, and imaging strategies
Joseph, Chacko, Quach, Julie M., Walkley, Carl R., Lane, Steven W., Celso, Cristina Lo and Purton, Louise E.. (2013). Deciphering hematopoietic stem cells in their niches: A critical appraisal of genetic models, lineage tracing, and imaging strategies. Cell Stem Cell. 13(5), pp. 520 - 533. https://doi.org/10.1016/j.stem.2013.10.010
The zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim
Jurado, Sabine, Gleeson, Kimberly, O’Donnell, Kristy, Izon, David J., Walkley, Carl R., Strasser, Andreas, Tarlinton, David M. and Heierhorst, Jörg. (2012). The zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim. Journal of Experimental Medicine. 209(9), pp. 1629-1639. https://doi.org/10.1084/jem.20120785
Fak depletion in both hematopoietic and nonhematopoietic niche cells leads to hematopoietic stem cell expansion
Lu, Jiayun, Sun, Yan, Nombela-Arrieta, Cesar, Du, Karrie P., Park, Shin-Young, Chai, Li, Walkley, Carl, Luo, Hongbo R. and Silberstein, Leslie E.. (2012). Fak depletion in both hematopoietic and nonhematopoietic niche cells leads to hematopoietic stem cell expansion. Experimental Hematology. 40(4), pp. 307-317. https://doi.org/10.1016/j.exphem.2011.11.010
AMPK phosphorylation of ACC2 is required for skeletal muscle fatty acid oxidation and insulin sensitivity in mice
O’Neill, Hayley M., Lally, James S., Galic, Sandra, Thomas, Melissa, Azizi, Paymon D., Fullerton, Morgan D., Smith, Brennan K., Pulinilkunnil, Thomas, Chen, Zhiping, Constantine Samaan, M., Jorgensen, Sebastian B., Dyck, Jason R. B., Holloway, Graham P., Hawke, Thomas J., van Denderen, Bryce J., Kemp, Bruce E. and Steinberg, Gregory R.. (2012). AMPK phosphorylation of ACC2 is required for skeletal muscle fatty acid oxidation and insulin sensitivity in mice. Diabetologia. 57(8), pp. 1693 - 1702. https://doi.org/10.1007/s00125-014-3273-1
AMPK-dependent inhibitory phosphorylation of ACC is not essential for maintaining myocardial fatty acid oxidation
Zordoky, Beshay N.M., Nagendran, Jeevan, Pulinilkunnil, Thomas, Kienesberger, Petra C., Masson, Grant, Waller, Terri J., Kemp, Bruce E., Steinberg, Gregory R. and Dyck, Jason R. B.. (2012). AMPK-dependent inhibitory phosphorylation of ACC is not essential for maintaining myocardial fatty acid oxidation. Circulation Research. 115(5), pp. 518 - 524. https://doi.org/10.1161/CIRCRESAHA.115.304538
The ancient drug salicylate directly activates AMP-activated protein kinase
Hawley, Simon, Fullerton, Morgan, Ross, Fiona, Schertzer, Jonathan, Chevtzoff, Cyrille, Walker, Katherine, Peggie, Mark, Zibrova, Darya, Green, Kevin, Mustard, Kirsty, Kemp, Bruce, Sakamoto, Kei, Steinberg, Gregory and Hardie, D. Grahame. (2012). The ancient drug salicylate directly activates AMP-activated protein kinase. Science. 336(6083), pp. 918 - 922. https://doi.org/10.1126/science.1215327
Pro-GRP-derived peptides are expressed in colorectal cancer cells and tumors and are biologically active in vivo
Patel, Oneel, Clyde, Daniel, Chang, Mike, Nordlund, Marianne, Steel, Rohan, Kemp, Bruce, Pritchard, D. Mark, Shulkes, Arthur and Baldwin, Graham. (2012). Pro-GRP-derived peptides are expressed in colorectal cancer cells and tumors and are biologically active in vivo. Endocrinology. 153(3), pp. 1082 - 1092. https://doi.org/10.1210/en.2011-1875
The outcome of renal ischemia-reperfusion injury is unchanged in AMPK-ß1 deficient mice
Mount, Peter, Gleich, Kurt, Tam, Shanna, Fraser, Scott, Choy, Suet-Wan, Dwyer, Karen, Lu, Bo, Van Denderen, Bryce, Fingerle-Rowson, Gunter, Bucala, Richard, Kemp, Bruce and Power, David. (2012). The outcome of renal ischemia-reperfusion injury is unchanged in AMPK-ß1 deficient mice. PLoS One (online). 7(1), pp. 1 - 10. https://doi.org/10.1371/journal.pone.0029887
Erythropoietin couples erythropoiesis, B-lymphopoiesis, and bone homeostasis within the bone marrow microenvironment
Singbrant, Sofie, Russell, Megan R., Jovic, Tanja, Liddicoat, Brian, Izon, David J., Purton, Louise E., Sims, Natalie A., Martin, T. John, Sankaran, Vijay G. and Walkley, Carl R.. (2011). Erythropoietin couples erythropoiesis, B-lymphopoiesis, and bone homeostasis within the bone marrow microenvironment. Blood. 117(21), pp. 5631-5642. https://doi.org/10.1182/blood-2010-11-320564
Erythropoiesis, anemia and the bone marrow microenvironment
Walkley, Carl R.. (2011). Erythropoiesis, anemia and the bone marrow microenvironment. International Journal of Hematology. 93, pp. 10-13. https://doi.org/10.1007/s12185-010-0759-6
Defining the hematopoietic stem cell niche : The chicken and the egg conundrum
Singbrant, Sofie, Askmyr, Maria, Purton, Louise E. and Walkley, Carl R.. (2011). Defining the hematopoietic stem cell niche : The chicken and the egg conundrum. Journal of Cellular Biochemistry. 112(6), pp. 1486-1490. https://doi.org/10.1002/jcb.23085
Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase
Alesultan, Ioana, Foller, Michael, Sopjani, Mentor, Dermaku-Sopjani, Miribane, Zelenak, Christine, Frohlich, Henning, Velic, Ana, Fraser, Scott, Kemp, Bruce, Seebohm, Guiscard, Volkl, Harald and Lang, Florian. (2011). Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase. Molecular Membrane Biology. 28(2), pp. 79 - 89. https://doi.org/10.3109/09687688.2010.520037
AMP-activated protein kinase (AMPK) ß1ß2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise
O'Neill, Hayley M., Maarbjerg, Stine J., Crane, Justin D., Jeppesen, Jacob, Jorgensen, Sebastian B., Schertzer, Jonathan D., Shyroka, Olga, Kiens, Bente, Van Denderen, Bryce J., Tarnopolsky, Mark A., Kemp, Bruce Ernest, Richter, Erik A. and Steinberg, Gregory R.. (2011). AMP-activated protein kinase (AMPK) ß1ß2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Proceedings of the National Academy of Sciences of USA. 108(38), pp. 16092 - 16097. https://doi.org/10.1073/pnas.1105062108
Ca 2+/calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation
Green, Michelle, Scott, John, Steel, Rohan, Oakhill, Jonathan, Kemp, Bruce and Means, Anthony. (2011). Ca 2+/calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation. Journal of Biological Chemistry. 286(32), pp. 28066 - 28079. https://doi.org/10.1074/jbc.M111.251504
Hematopoietic AMPK Beta ß1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity
Galic, Sandra, Fullerton, Morgan D., Schertzer, Jonathan D., Sikkema, Sarah, Marcinko, Katarina, Walkley, Carl R., Izon, David, Honeyman, Jane, Chen, Zhi-Ping, van Denderen, Bryce J., Kemp, Bruce Ernest and Steinberg, Gregory R.. (2011). Hematopoietic AMPK Beta ß1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. Journal of Clinical Investigation. 121(12), pp. 4903 - 4915. https://doi.org/10.1172/JCI58577
Inhibition of Kir2.1 (KCNJ2) by the AMP-activated protein kinase
Alesutan, Ioana, Munoz, Carlos, Sopjani, Mentor, Dermaku-Sopjani, Miribane, Michael, Diana, Fraser, Scott, Kemp, Bruce, Seebohm, Guiscard, Foller, Michael and Lang, Florian. (2011). Inhibition of Kir2.1 (KCNJ2) by the AMP-activated protein kinase. Biochemical and Biophysical Research Communications. 408(4), pp. 505 - 510. https://doi.org/10.1016/j.bbrc.2011.04.015
Inhibition of Connexin 26 by the AMP-activated protein kinase
Alesutan, Ioana, Sopjani, Mentor, Munoz, Carlos, Fraser, Scott, Kemp, Bruce, Foller, Michael and Lang, Florian. (2011). Inhibition of Connexin 26 by the AMP-activated protein kinase. Journal of Membrane Biology: an international journal for studies on the structure, function and genesis of biomembranes. 240(3), pp. 151 - 158. https://doi.org/10.1007/s00232-011-9353-y
AMPK is a direct adenylate charge-regulated protein kinase
Oakhill, Jonathan S., Steel, Rohan, Chen, Zhi-Ping, Scott, John W., Ling, Naomi, Tam, Shanna and Kemp, Bruce Ernest. (2011). AMPK is a direct adenylate charge-regulated protein kinase. Science. 332(6036), pp. 1433 - 1435. https://doi.org/10.1126/science.1200094
AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1
Fraser, Scott A., Choy, Suet-Wan, Pastor-Soler, Núria M., Li, Hui, Davies, Matthew R. P., Cook, Natasha, Katerelos, Marina, Mount, Peter F., Gleich, Kurt, McRae, Jennifer L., Dwyer, Karen M., van Denderen, Bryce J. W., Hallows, Kenneth R., Kemp, Bruce E. and Power, David A.. (2010). AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1. American Journal of Physiology - Renal Physiology. 305(5), pp. F679 - F690. https://doi.org/10.1152/ajprenal.00407.2012
Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated with whole-body insulin resistance in rats bred for low intrinsic exercise capacity
Lessard, Sarah J., Rivas, Donato A., Chen, Zhu-Ping, van Denderen, Bryce J., Watt, Matthew J., Koch, Lauren G., Britton, Steven L., Kemp, Bruce Ernest and Hawley, John Alan. (2009). Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated with whole-body insulin resistance in rats bred for low intrinsic exercise capacity. Endocrinology. 150(11), pp. 4883 - 4891. https://doi.org/10.1210/en.2009-0158
Fat adaptation followed by carbohydrate restoration increases AMPK activity in skeletal muscle from trained humans
Yeo, Wee Kian, Lessard, Sarah J., Chen, Zhi-Ping, Garnham, Andrew P., Burke, Louise, Rivas, Donato A., Kemp, Bruce Ernest and Hawley, John Alan. (2008). Fat adaptation followed by carbohydrate restoration increases AMPK activity in skeletal muscle from trained humans. Journal of Applied Physiology. 105(5), pp. 1519 - 1526. https://doi.org/10.1152/japplphysiol.90540.2008
Activation of AMPK reduces the co-transporter activity of NKCC1
Fraser, Scott A., Davies, Matthew, Katerelos, Marina, Gleich, Kurt, Choy, Suet-Wan, Steel, Rohan, Galic, Sandra, Mount, Peter F., Kemp, Bruce E. and Power, David A.. (1999). Activation of AMPK reduces the co-transporter activity of NKCC1. Molecular Membrane Biology. 31(2-3), pp. 95 - 102. https://doi.org/10.3109/09687688.2014.902128
Myosin light chain kinases
Kemp, Bruce E. and Stull, James T.. (1990). Myosin light chain kinases. In In B. E. Kemp (Ed.). Peptides and Protein Phosphorylation pp. 115 - 133 CRC Press. https://doi.org/10.1201/9781351075442