Herbicide impact on Hormosira banksii gametes measured by fluorescence and germination bioassays

Journal article


Seery, Clifford R., Gunthorpe, Leanne and Ralph, Peter J.. (2006) Herbicide impact on Hormosira banksii gametes measured by fluorescence and germination bioassays. Environmental Pollution. 140(1), pp. 43 - 51. https://doi.org/10.1016/j.envpol.2005.07.001
AuthorsSeery, Clifford R., Gunthorpe, Leanne and Ralph, Peter J.
Abstract

The innovative bioassay described here involves chlorophyll a fluorescence measurements of gametes from the macroalgae, Hormosira banksii, where gametes (eggs) were exposed to Diuron, Irgarol and Bromacil. Response was assessed as percent inhibition from control of effective quantum yield (ΔF/Fm′) of photosystem II, herein referred to as % PSII Inhibition. This was measured with the dual-channelled pulse amplitude modulated (PAM) fluorometer, ToxY-PAM. The fluorescence bioassay was run simultaneously with an established H. banksii germination bioassay to compare sensitivity, precision, and time-to-result. The fluorescence bioassay gave highly sensitive results evidenced by EC50s (% PSII Inhibition) for Diuron, Irgarol and Bromacil being three, four and three orders of magnitude (respectively) lower than EC50s generated from the germination bioassays. Precision of the fluorescence bioassay was demonstrated with low coefficient of variations ( < 30%) for all three toxicants. With regard to time, the fluorescence bioassay gave results within 6 h, as opposed to more than 50 h for the germination bioassay.

Keywordschlorophyll a fluorescence; ToxY-PAM; toxicity bioassay; macroalgae; hormosira banksii
Year2006
JournalEnvironmental Pollution
Journal citation140 (1), pp. 43 - 51
PublisherPergamon
ISSN0269-7491
Digital Object Identifier (DOI)https://doi.org/10.1016/j.envpol.2005.07.001
Page range43 - 51
Research GroupSchool of Behavioural and Health Sciences
Place of publicationUnited Kingdom
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https://acuresearchbank.acu.edu.au/item/88v60/herbicide-impact-on-hormosira-banksii-gametes-measured-by-fluorescence-and-germination-bioassays

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