Pan-proteomics, a concept for unifying quantitative proteome measurements when comparing closely-related bacterial strains

Journal article


Broadbent, James A., Broszczak, Daniel A., Tennakoon, Imalka U. K. and Huygens, Flavia. (2016) Pan-proteomics, a concept for unifying quantitative proteome measurements when comparing closely-related bacterial strains. Expert Review of Proteomics. 13(4), pp. 355 - 365. https://doi.org/10.1586/14789450.2016.1155986
AuthorsBroadbent, James A., Broszczak, Daniel A., Tennakoon, Imalka U. K. and Huygens, Flavia
Abstract

The comparison of proteomes between genetically heterogeneous bacterial strains may offer valuable insights into physiological diversity and function, particularly where such variation aids in the survival and virulence of clinically-relevant strains. However, reports of such comparisons frequently fail to account for underlying genetic variance. As a consequence, the current knowledge regarding bacterial physiological diversity at the protein level may be incomplete or inaccurate. To address this, greater consideration must be given to the impact of genetic heterogeneity on proteome comparisons. This may be possible through the use of pan-proteomics, an analytical concept that permits the ability to qualitatively and quantitatively compare the proteomes of genetically heterogeneous organisms. Limited examples of this emerging technology highlight currently unmet analytical challenges. In this article we define pan-proteomics, where its value lies in microbiology, and discuss the technical considerations critical to its successful execution and potential future application.

Keywordspan-proteomics; microbiology; bioinformatics; bacterial strains; comparative proteomics; genetic heterogeneity; clinical microbiology
Year2016
JournalExpert Review of Proteomics
Journal citation13 (4), pp. 355 - 365
PublisherTaylor and Francis Ltd
ISSN1478-9450
Digital Object Identifier (DOI)https://doi.org/10.1586/14789450.2016.1155986
Scopus EID2-s2.0-84961217145
Page range355 - 365
Research GroupSchool of Behavioural and Health Sciences
Place of publicationUnited Kingdom
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