The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells

Journal article


Joglekar, Mugdha V., Patil, Deepak, Joglekar, Vinay M., Rao, Guduru Venkat, Reddy, Nageshwar Duvvuru, Mitnala, Sasikala, Shouche, Yogesh and Hardikar, Anandwardhan. (2009). The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells. Islets. 1(2), pp. 137 - 147. https://doi.org/10.4161/isl.1.2.9578
AuthorsJoglekar, Mugdha V., Patil, Deepak, Joglekar, Vinay M., Rao, Guduru Venkat, Reddy, Nageshwar Duvvuru, Mitnala, Sasikala, Shouche, Yogesh and Hardikar, Anandwardhan
Abstract

Epithelial-to-mesenchymal transition is a phenomenon necessary for embryonic development and also seen during certain pathological conditions. We show here for the first time that reduction in miR-30 family microRNAs, is responsible for mesenchymal transition of primary cultures of human pancreatic epithelial cells. We found that miR-30 family microRNAs target mesenchymal gene transcripts and maintain them in a translationally inactive state. Forced depletion using miR-30 family specific anti-miRs leads to mesenchymal transition while ectopic overexpression maintains the epithelial phenotype. We also show that miR-30 family microRNAs increase in abundance during differentiation of pancreatic islet-derived mesenchymal cells into hormone-producing islet-like cell aggregates. Our studies in human adult diseased pancreas also demonstrate that miR-30 family microRNAs are expressed at lower abundance in fibrotic lesions during pancreatitis. Together, our data confirm that miR-30 family microRNAs form a part of the regulatory signaling events involved in cellular response of pancreatic epithelial cells during mesenchymal transition.

KeywordsmicroRNA; miR-30; EMT; pancreas; islet
Year2009
JournalIslets
Journal citation1 (2), pp. 137 - 147
PublisherLandes Bioscience
ISSN1938-2014
Digital Object Identifier (DOI)https://doi.org/10.4161/isl.1.2.9578
Page range137 - 147
Research GroupSchool of Nursing, Midwifery and Paramedicine
Place of publicationUnited States of America
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