Effects of acute exercise on lipid content and dietary lipid uptake in liver and skeletal muscle of lean and diabetic rats

Journal article


Janssens, Sharon, Jonkers, Richard A. M., Groen, Albert K., Nicolay, Klaas, Van Loon, Luc J. C. and Prompers, Jeanine J.. (2015) Effects of acute exercise on lipid content and dietary lipid uptake in liver and skeletal muscle of lean and diabetic rats. American Journal of Physiology - Endocrinology and Metabolism. 309(10), pp. 874 - 883. https://doi.org/10.1152/ajpendo.00292.2015
AuthorsJanssens, Sharon, Jonkers, Richard A. M., Groen, Albert K., Nicolay, Klaas, Van Loon, Luc J. C. and Prompers, Jeanine J.
Abstract

Insulin resistance is associated with ectopic lipid accumulation. Physical activity improves insulin sensitivity, but the impact of exercise on lipid handling in insulin-resistant tissues remains to be elucidated. The present study characterizes the effects of acute exercise on lipid content and dietary lipid partitioning in liver and skeletal muscle of lean and diabetic rats by use of magnetic resonance spectroscopy ( MRS ). After baseline measurements, rats were randomized to exercise or no-exercise groups. A subset of animals was subjected to MRS directly after 1 h of treadmill running for measurement of total intrahepatocellular lipid ( IHCL ) and intramyocellular lipid ( IMCL ) content ( n = 7 lean and diabetic rats ). The other animals were administered 13C-labeled lipids orally after treadmill visit ( with or without exercise ) followed by MRS measurements after 4 and 24 h to determine the 13C enrichment of IHCL and IMCL ( n = 8 per group ). Total IHCL and IMCL content were fivefold higher in diabetic vs. lean rats ( P < 0.001 ). Exercise did not significantly affect IHCL content but reduced IMCL by 25 ± 7 and 33 ± 4% in lean and diabetic rats ( P < 0.05 ), respectively. Uptake of dietary lipids in liver and muscle was 2.3-fold greater in diabetic vs. lean rats ( P < 0.05 ). Prior exercise did not significantly modulate dietary lipid uptake into muscle, but in liver of both lean and diabetic rats, lipid uptake was 44% reduced after acute exercise ( P < 0.05 ). In conclusion, IMCL but not IHCL represents a viable substrate source during exercise in both lean and diabetic rats, and exercise differentially affects dietary lipid uptake in muscle and liver.

Year2015
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Journal citation309 (10), pp. 874 - 883
PublisherAmerican Physiological Society
ISSN0193-1849
Digital Object Identifier (DOI)https://doi.org/10.1152/ajpendo.00292.2015
Scopus EID2-s2.0-84947292688
Page range874 - 883
Research GroupMary MacKillop Institute for Health Research
Publisher's version
File Access Level
Controlled
Place of publicationUnited States
Permalink -

https://acuresearchbank.acu.edu.au/item/89vx5/effects-of-acute-exercise-on-lipid-content-and-dietary-lipid-uptake-in-liver-and-skeletal-muscle-of-lean-and-diabetic-rats

Restricted files

Publisher's version

  • 3
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month
These values are for the period from 19th October 2020, when this repository was created.

Export as