Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes

Journal article


Shaw, C. S., Shepherd, Sam O., Wagenmakers, A. J. M., Hansen, Dominique, Dendale, Paul and Van Loon, Luc J.C. (2012). Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes. American Journal of Physiology - Endocrinology and Metabolism. 303(9), pp. 1158 - 1165. https://doi.org/10.1152/ajpendo.00272.2012
AuthorsShaw, C. S., Shepherd, Sam O., Wagenmakers, A. J. M., Hansen, Dominique, Dendale, Paul and Van Loon, Luc J.C
Abstract

The aim of the present study was to investigate changes in intramuscular triglyceride ( IMTG ) content and perilipin 2 expression in skeletal muscle tissue following 6 mo of endurance-type exercise training in type 2 diabetes patients. Ten obese male type 2 diabetes patients ( age 62 ± 1 yr, body mass index BMI 31 ± 1 kg/m2 ) completed three exercise sessions/week consisting of 40 min of continuous endurance-type exercise at 75% V̇o2 peak for a period of 6 mo. Muscle biopsies collected at baseline and after 2 and 6 mo of intervention were analyzed for IMTG content and perilipin 2 expression using fiber type-specific immunofluorescence microscopy. Endurance-type exercise training reduced trunk body fat by 6 ± 2% and increased whole body oxygen uptake capacity by 13 ± 7% ( P < 0.05 ). IMTG content increased twofold in response to the 6 mo of exercise training in both type I and type II muscle fibers ( P < 0.05 ). A threefold increase in perilipin 2 expression was observed from baseline to 2 and 6 mo of intervention in the type I muscle fibers only ( 1.1 ± 0.3, 3.4 ± 0.6, and 3.6 ± 0.6% of fibers stained, respectively, P < 0.05 ). Exercise training induced a 1.6-fold increase in mitochondrial content after 6 mo of training in both type I and type II muscle fibers ( P < 0.05 ). In conclusion, this is the first study to report that prolonged endurance-type exercise training increases the expression of perilipin 2 alongside increases in IMTG content in a type I muscle fiber-type specific manner in type 2 diabetes patients.

Year2012
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Journal citation303 (9), pp. 1158 - 1165
PublisherAmerican Physiological Society
ISSN0193-1849
Digital Object Identifier (DOI)https://doi.org/10.1152/ajpendo.00272.2012
Scopus EID2-s2.0-84868326874
Page range1158 - 1165
Research GroupMary MacKillop Institute for Health Research
Publisher's version
File Access Level
Controlled
Place of publicationUnited States
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