Exercise and GLUT4 in human subcutaneous adipose tissue
Journal article
Flores-Opazo, Marcelo, Boland, Eva, Garnham, Andrew, Murphy, Robyn M., McGee, Sean L. and Hargreaves, Mark. (2018). Exercise and GLUT4 in human subcutaneous adipose tissue. Physiological Reports. 6(22), pp. 1 - 8. https://doi.org/10.14814/phy2.13918
Authors | Flores-Opazo, Marcelo, Boland, Eva, Garnham, Andrew, Murphy, Robyn M., McGee, Sean L. and Hargreaves, Mark |
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Abstract | To examine the effect of acute and chronic exercise on adipose tissue GLUT4 expression, a total of 20 healthy, male subjects performed one of two studies. Ten subjects performed cycle ergometer exercise for 60 min at 73 ± 2% VO2 peak and abdominal adipose tissue samples were obtained immediately before and after exercise and after 3 h of recovery. Another 10 subjects completed 10 days of exercise training, comprising a combination of six sessions of 60 min at 75% VO2 peak and four sessions of 6 × 5 min at 90% VO2 peak, separated by 3 min at 40% VO2 peak. Abdominal adipose tissue and vastus lateralis muscle samples were obtained before training and 24 h after the last training session. A single bout of exercise did not change adipose tissue GLUT4 mRNA; however, there was a small, but significant, reduction in adipose tissue GLUT4 protein expression 3 h after exercise. There were no changes in adipose tissue GLUT4 or COX‐IV expression following exercise training. In contrast, skeletal muscle GLUT4 and COX‐IV were increased by 47% and 44%, respectively following exercise training. The exercise training‐induced increase in GLUT4 expression was similar in both type I and type IIa single muscle fibers. Our results indicate that neither a single exercise bout, nor 10 days of exercise training, increased adipose tissue GLUT4, in contrast with the increases observed in skeletal muscle GLUT4 expression. |
Keywords | Adipose tissue; exercise; GLUT4; skeletal muscle |
Year | 2018 |
Journal | Physiological Reports |
Journal citation | 6 (22), pp. 1 - 8 |
Publisher | John Wiley & Sons |
ISSN | 2051-817X |
Digital Object Identifier (DOI) | https://doi.org/10.14814/phy2.13918 |
Scopus EID | 2-s2.0-85056692700 |
Open access | Open access |
Page range | 1 - 8 |
Research Group | Mary MacKillop Institute for Health Research |
Publisher's version | License |
Place of publication | United States of America |
Editors | T. Kleyman |
https://acuresearchbank.acu.edu.au/item/8q1xy/exercise-and-glut4-in-human-subcutaneous-adipose-tissue
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OA_Flores_Opazo_2018_Exercise_and_GLUT4_in_human_subcutaneous.pdf | |
License: CC BY 4.0 |
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