Identification of traditional medicinal plant extracts with novel anti-influenza activity

Journal article


Rajasekaran, Dhivya, Palombo, Enzo, Yeo, Tiong Chia, Ley, Diana, Tu, Chu, Malherbe, Francois and Grollo, Lara. (2013). Identification of traditional medicinal plant extracts with novel anti-influenza activity. PLoS One (online). 8(11), pp. 1 - 15. https://doi.org/10.1371/journal.pone.0079293
AuthorsRajasekaran, Dhivya, Palombo, Enzo, Yeo, Tiong Chia, Ley, Diana, Tu, Chu, Malherbe, Francois and Grollo, Lara
Abstract

The emergence of drug resistant variants of the influenza virus has led to a need to identify novel and effective antiviral agents. As an alternative to synthetic drugs, the consolidation of empirical knowledge with ethnopharmacological evidence of medicinal plants offers a novel platform for the development of antiviral drugs. The aim of this study was to identify plant extracts with proven activity against the influenza virus. Extracts of fifty medicinal plants, originating from the tropical rainforests of Borneo used as herbal medicines by traditional healers to treat flu-like symptoms, were tested against the H1N1 and H3N1 subtypes of the virus. In the initial phase, in vitro micro-inhibition assays along with cytotoxicity screening were performed on MDCK cells. Most plant extracts were found to be minimally cytotoxic, indicating that the compounds linked to an ethnomedical framework were relatively innocuous, and eleven crude extracts exhibited viral inhibition against both the strains. All extracts inhibited the enzymatic activity of viral neuraminidase and four extracts were also shown to act through the hemagglutination inhibition (HI) pathway. Moreover, the samples that acted through both HI and neuraminidase inhibition (NI) evidenced more than 90% reduction in virus adsorption and penetration, thereby indicating potent action in the early stages of viral replication. Concurrent studies involving Receptor Destroying Enzyme treatments of HI extracts indicated the presence of sialic acid-like component(s) that could be responsible for hemagglutination inhibition. The manifestation of both modes of viral inhibition in a single extract suggests that there may be a synergistic effect implicating more than one active component. Overall, our results provide substantive support for the use of Borneo traditional plants as promising sources of novel anti-influenza drug candidates. Furthermore, the pathways involving inhibition of hemagglutination could be a solution to the global occurrence of viral strains resistant to neuraminidase drugs.

Year2013
JournalPLoS One (online)
Journal citation8 (11), pp. 1 - 15
ISSN1932-6203
Digital Object Identifier (DOI)https://doi.org/10.1371/journal.pone.0079293
Open accessOpen access
Page range1 - 15
Research GroupSchool of Behavioural and Health Sciences
Publisher's version
Permalink -

https://acuresearchbank.acu.edu.au/item/8q21v/identification-of-traditional-medicinal-plant-extracts-with-novel-anti-influenza-activity

  • 70
    total views
  • 91
    total downloads
  • 2
    views this month
  • 2
    downloads this month
These values are for the period from 19th October 2020, when this repository was created.

Export as

Related outputs

Salivary blockade protects the lower respiratory tract of mice from lethal influenza virus infection
Ivinson, Karen, Deliyannis, Georgia, McNabb, Leanne, Grollo, Lara, Gilbertson, Brad, Jackson, David and Brown, Lorena E.. (2017). Salivary blockade protects the lower respiratory tract of mice from lethal influenza virus infection. Journal of Virology. 91(14), pp. 1 - 12. https://doi.org/10.1128/JVI.00624-17
Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
Ramanathan, Babu, Poh, Chit L., Kirk, Kristin, McBride, William John Hannan, Aaskov, John and Grollo, Lara. (2016). Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLoS ONE. 11(5), pp. 1 - 22. https://doi.org/10.1371/journal.pone.0155900
Enterovirus-specific anti-peptide antibodies
Poh, Chit Laa, Kirk, Katherine, Chua, Hui Na and Grollo, Lara. (2015). Enterovirus-specific anti-peptide antibodies. In In Houen, Gunnar (Ed.). Peptide antibodies methods and protocols pp. 341-350 Humana Press. https://doi.org/10.1007/978-1-4939-2999-3_29
Development of antiviral agents toward enterovirus 71 infection
Pourianfar, Hamid Reza and Grollo, Lara. (2015). Development of antiviral agents toward enterovirus 71 infection. Journal of Microbiology Immunology and Infection. 48(1), pp. 1 - 8. https://doi.org/10.1016/j.jmii.2013.11.011
Evidence of synergistic activity of medicinal plant extracts against neuraminidase inhibitor resistant strains of influenza
Rajasekaran, Dhivya, Palombo, Enzo, Yeo, Tiong Chia, Ley, Diana, Tu, Chu Lee, Malherbe, Francois and Grollo, Lara. (2014). Evidence of synergistic activity of medicinal plant extracts against neuraminidase inhibitor resistant strains of influenza. Advances in Microbiology. 4(16), pp. 1260 - 1277. https://doi.org/10.4236/aim.2014.416136
Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface hepaan sulphate
Pourianfar, Hamid Reza, Kirk, Kristin and Grollo, Lara. (2014). Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface hepaan sulphate. VirusDisease. 25(3), pp. 277 - 284. https://doi.org/10.1007/s13337-013-0172-x
Global impact of heparin on gene expression profiles in neural cells infected by Enterovirus 71
Pourianfar, H. R., Palombo, E. and Grollo, L.. (2014). Global impact of heparin on gene expression profiles in neural cells infected by Enterovirus 71. Intervirology: international journal of basic and medical virology. 57(2), pp. 93 - 100. https://doi.org/10.1159/000355872
In vitro evaluation of the antiviral activity of heparan sulfate mimetic compounds against Enterovirus 71
Pourianfar, Hamid, Poh, Chit Laa, Fecondo, John and Grollo, Lara. (2012). In vitro evaluation of the antiviral activity of heparan sulfate mimetic compounds against Enterovirus 71. Virus Research. 169(1), pp. 22 - 29. https://doi.org/10.1016/j.virusres.2012.06.025
A colorimetric-based accurate method for the determination of enterovirus 71 titer
Pourianfar, Hamid Reza, Javadi, Arman and Grollo, Lara. (2012). A colorimetric-based accurate method for the determination of enterovirus 71 titer. Indian Journal of Virology. 23(3), pp. 303 - 310. https://doi.org/10.1007/s13337-012-0105-0
Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach
Kirk, Kristin, Poh, Chit Lau, Fecondo, John, Shaw, Jillian and Grollo, Lara. (2012). Cross-reactive neutralizing antibody epitopes against Enterovirus 71 identified by an in silico approach. Vaccine. 30(49), pp. 7105 - 7110. https://doi.org/10.1016/j.vaccine.2012.09.030