Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine

Journal article


Ramanathan, Babu, Poh, Chit L., Kirk, Kristin, McBride, William John Hannan, Aaskov, John and Grollo, Lara. (2016). Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLoS ONE. 11(5), pp. 1 - 22. https://doi.org/10.1371/journal.pone.0155900
AuthorsRamanathan, Babu, Poh, Chit L., Kirk, Kristin, McBride, William John Hannan, Aaskov, John and Grollo, Lara
Abstract

Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine.

Year2016
JournalPLoS ONE
Journal citation11 (5), pp. 1 - 22
PublisherPublic Library of Science
ISSN1932-6203
Digital Object Identifier (DOI)https://doi.org/10.1371/journal.pone.0155900
Scopus EID2-s2.0-84971260873
Open accessOpen access
Page range1 - 22
Research GroupSchool of Behavioural and Health Sciences
Publisher's version
License
Additional information

© 2016 Ramanathan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Place of publicationUnited States of America
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https://acuresearchbank.acu.edu.au/item/8q32x/synthetic-b-cell-epitopes-eliciting-cross-neutralizing-antibodies-strategies-for-future-dengue-vaccine

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