Versatile wetting measurement of microplate wells

Journal article

Ng, Enoch Ming Wei, Cheong, Brandon Huey-Ping, Yu, Yang, Liew, Oi Wah and Ng, Tuck Wah. (2016). Versatile wetting measurement of microplate wells. Review of Scientific Instruments. 87(11), pp. 115107-1 - 115107-7. https://doi.org/10.1063/1.4965038
AuthorsNg, Enoch Ming Wei, Cheong, Brandon Huey-Ping, Yu, Yang, Liew, Oi Wah and Ng, Tuck Wah
Abstract

A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4–6 μl showed no measured variance in the contact angle. Experiments with phosphate buffer solution with varied concentrations of a non-ionic detergent (Tween 20) dissolved, however, revealed smaller contact angles with increased detergent concentration. It is experimentally shown that drops can be located up to 7° from the lowest position of the well without affecting the accuracy of contact angle measurements. Numerical simulations confirm the ability of the drops to manifest the correct contact angle despite the lack of axis-symmetry in their shape while residing on a circular surface. This method offers a convenient means to determine the wetting characteristics of different liquid samples in different microplates.

Year2016
JournalReview of Scientific Instruments
Journal citation87 (11), pp. 115107-1 - 115107-7
PublisherAmerican Institute of Physics
ISSN0034-6748
Digital Object Identifier (DOI)https://doi.org/10.1063/1.4965038
Scopus EID2-s2.0-84994850718
Page range115107-1 - 115107-7
Research GroupSchool of Behavioural and Health Sciences
Publisher's version
File Access Level
Controlled
Place of publicationUnited States of America
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