Versatile wetting measurement of microplate wells
Journal article
Ng, Enoch Ming Wei, Cheong, Brandon Huey-Ping, Yu, Yang, Liew, Oi Wah and Ng, Tuck Wah. (2016). Versatile wetting measurement of microplate wells. Review of Scientific Instruments. 87(11), pp. 115107-1 - 115107-7. https://doi.org/10.1063/1.4965038
Authors | Ng, Enoch Ming Wei, Cheong, Brandon Huey-Ping, Yu, Yang, Liew, Oi Wah and Ng, Tuck Wah |
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Abstract | A method to measure the contact angle, which is indicative of wetting, using small liquid volumes dispensed directly on microplate wells is described and demonstrated. Experiments with enhanced green protein samples of volumes 4.4–6 μl showed no measured variance in the contact angle. Experiments with phosphate buffer solution with varied concentrations of a non-ionic detergent (Tween 20) dissolved, however, revealed smaller contact angles with increased detergent concentration. It is experimentally shown that drops can be located up to 7° from the lowest position of the well without affecting the accuracy of contact angle measurements. Numerical simulations confirm the ability of the drops to manifest the correct contact angle despite the lack of axis-symmetry in their shape while residing on a circular surface. This method offers a convenient means to determine the wetting characteristics of different liquid samples in different microplates. |
Year | 2016 |
Journal | Review of Scientific Instruments |
Journal citation | 87 (11), pp. 115107-1 - 115107-7 |
Publisher | American Institute of Physics |
ISSN | 0034-6748 |
Digital Object Identifier (DOI) | https://doi.org/10.1063/1.4965038 |
Scopus EID | 2-s2.0-84994850718 |
Page range | 115107-1 - 115107-7 |
Research Group | School of Behavioural and Health Sciences |
Publisher's version | File Access Level Controlled |
Place of publication | United States of America |
https://acuresearchbank.acu.edu.au/item/8q76q/versatile-wetting-measurement-of-microplate-wells
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