Cell culture metabolomics in the diagnosis of lung cancer - The influence of cell culture conditions

Journal article


Kalluri, U., Naiker, Mani and Myers, M. A.. (2014) Cell culture metabolomics in the diagnosis of lung cancer - The influence of cell culture conditions. Journal of Breath Research. 8(2), pp. 1 - 10. https://doi.org/10.1088/1752-7155/8/2/027109
AuthorsKalluri, U., Naiker, Mani and Myers, M. A.
Abstract

Lung cancer is the leading cause of cancer deaths. Unfortunately, lung cancer is often diagnosed only when it becomes symptomatic or at an advanced stage when few treatment options are available. Hence, a diagnostic test suitable for screening widespread populations is required to enable earlier diagnosis. Analysis of exhaled breath provides a non-invasive method for early detection of lung cancer. Analysis of volatile organic compounds (VOCs) by various mass spectral techniques has identified potential biomarkers of disease. Nevertheless, the metabolic origins and the disease specificity of VOCs need further elucidation. Cell culture metabolomics can be used as a bottom-up approach to identify biomarkers of pathological conditions and can also be used to study the metabolic pathways that produce such compounds. This paper summarizes the current knowledge of lung cancer biomarkers in exhaled breath and emphasizes the critical role of cell culture conditions in determining the VOCs produced in vitro. Hypoxic culture conditions more closely mimic the conditions of cancer cell growth in vivo. We propose that since hypoxia influences cell metabolism and so potentially the VOCs that the cancer cells produce, the cell culture metabolomics projects should consider culturing cancer cells in hypoxic conditions.

Year2014
JournalJournal of Breath Research
Journal citation8 (2), pp. 1 - 10
PublisherInstitute of Physics Publishing
ISSN1752-7155
Digital Object Identifier (DOI)https://doi.org/10.1088/1752-7155/8/2/027109
Scopus EID2-s2.0-84901628702
Open accessOpen access
Page range1 - 10
Research GroupSchool of Behavioural and Health Sciences
Publisher's version
Additional information

Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

Place of publicationUnited Kingdom
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