The muscle protein synthetic response to whey protein ingestion is greater in middle-aged women when compared with men

Journal article


Horstman, Astrid M. H., Kouw, Imre W. K., van Dijk, Jan-Willem, Hamer, Henrike M., Groen, Bart B. L., van Kranenburg, Janneau, Gorissen, Stefan H. M. and van Loon, Luc J. C.. (2019) The muscle protein synthetic response to whey protein ingestion is greater in middle-aged women when compared with men. Journal of Clinical Endocrinology and Metabolism. 104(4), pp. 994 - 1004. https://doi.org/10.1210/jc.2018-01734
AuthorsHorstman, Astrid M. H., Kouw, Imre W. K., van Dijk, Jan-Willem, Hamer, Henrike M., Groen, Bart B. L., van Kranenburg, Janneau, Gorissen, Stefan H. M. and van Loon, Luc J. C.
Abstract

Rationale: Muscle mass maintenance is largely regulated by the postprandial rise in muscle protein synthesis rates. It remains unclear whether postprandial protein handling differs between women and men. Methods: Healthy men (43 ± 3 years; body mass index, 23.4 ± 0.4 kg/m2; n = 12) and women (46 ± 2 years; body mass index, 21.3 ± 0.5 kg/m2; n = 12) received primed continuous infusions of L-[ring-2H5]-phenylalanine and L-[ring-3,5-2H2]-tyrosine and ingested 25 g intrinsically L-[1-13C]-phenylalanine–labeled whey protein. Blood samples and muscle biopsies were collected to assess dietary protein digestion and amino acid absorption kinetics as well as basal and postprandial myofibrillar protein synthesis rates. Results: Plasma phenylalanine and leucine concentrations rapidly increased after protein ingestion (both P < 0.001), with no differences between middle-aged women and men (Time × Sex, P = 0.307 and 0.529, respectively). The fraction of dietary protein–derived phenylalanine that appeared in the circulation over the 5-hour postprandial period averaged 56 ± 1% and 53 ± 1% in women and men, respectively (P = 0.145). Myofibrillar protein synthesis rates increased (Time, P = 0.010) from 0.035 ± 0.004%/h and 0.030 ± 0.002%/h in the postabsorptive state (t test, P = 0.319) to 0.045 ± 0.002%/h and 0.034 ± 0.002%/h in the 5-hour postprandial phase in middle-aged women and men, respectively, with higher postprandial myofibrillar protein synthesis rates in women compared with men (ttest, P = 0.005). Middle-aged women showed a greater increase in myofibrillar protein synthesis rates during the early (0 to 2 hours) postprandial period compared with men (Time × Sex, P = 0.001). Conclusions: There are no differences in postabsorptive myofibrillar protein synthesis rates between middle-aged women and men. The myofibrillar protein synthetic response to the ingestion of 25 g whey protein is greater in women than in men.

Year2019
JournalJournal of Clinical Endocrinology and Metabolism
Journal citation104 (4), pp. 994 - 1004
PublisherOxford University Press
ISSN0021-972X
Digital Object Identifier (DOI)https://doi.org/10.1210/jc.2018-01734
Scopus EID2-s2.0-85061492438
Open accessPublished as green open access
Page range994 - 1004
Research GroupMary MacKillop Institute for Health Research
Author's accepted manuscript
Publisher's version
File Access Level
Controlled
Additional information

This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Clinical Endocrinology & Metabolism following peer review. The version of record Astrid M H Horstman, Imre W K Kouw, Jan-Willem van Dijk, Henrike M Hamer, Bart B L Groen, Janneau van Kranenburg, Stefan H M Gorissen, Luc J C van Loon, The Muscle Protein Synthetic Response to Whey Protein Ingestion Is Greater in Middle-Aged Women Compared With Men, The Journal of Clinical Endocrinology & Metabolism, Volume 104, Issue 4, April 2019, Pages 994–1004 is available online at: http://doi.org/10.1210/jc.2018-01734.

Place of publicationUnited Kingdom
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