Detection of localized hepatocellular amino acid kinetics by using mass spectrometry imaging of stable isotopes
Journal article
Arts, Martijn, Soons, Zita, Ellis, Shane R., Pierzchalski, Keely A., Balluff, Benjamin, Eijkel, Gert B., Dubois, Ludwig J., Lieuwes, Natasja G., Agten, Stijn M., Hackeng, Tilman M., Van Loon, Luc, Heeren, Ron M.A. and Damink, Steven W.O.. (2017). Detection of localized hepatocellular amino acid kinetics by using mass spectrometry imaging of stable isotopes. Angewandte Chemie. 56(25), pp. 7146 - 7150. https://doi.org/10.1002/anie.201702669
Authors | Arts, Martijn, Soons, Zita, Ellis, Shane R., Pierzchalski, Keely A., Balluff, Benjamin, Eijkel, Gert B., Dubois, Ludwig J., Lieuwes, Natasja G., Agten, Stijn M., Hackeng, Tilman M., Van Loon, Luc, Heeren, Ron M.A. and Damink, Steven W.O. |
---|---|
Abstract | Mass spectrometry imaging (MSI) simultaneously detects and identifies the spatial distribution of numerous molecules throughout tissues. Currently, MSI is limited to providing a static and ex vivo snapshot of highly dynamic systems in which molecules are constantly synthesized and consumed. Herein, we demonstrate an innovative MSI methodology to study dynamic molecular changes of amino acids within biological tissues by measuring the dilution and conversion of stable isotopes in a mouse model. We evaluate the method specifically on hepatocellular metabolism of the essential amino acid l-phenylalanine, associated with liver diseases. Crucially, the method reveals the localized dynamics of l-phenylalanine metabolism, including its in vivo hydroxylation to l-tyrosine and co-localization with other liver metabolites in a time course of samples from different animals. This method thus enables the dynamics of localized biochemical synthesis to be studied directly from biological tissues. |
Year | 2017 |
Journal | Angewandte Chemie |
Journal citation | 56 (25), pp. 7146 - 7150 |
Publisher | Wiley |
ISSN | 1433-7851 |
Digital Object Identifier (DOI) | https://doi.org/10.1002/anie.201702669 |
Scopus EID | 2-s2.0-85019167252 |
Page range | 7146 - 7150 |
Research Group | Mary MacKillop Institute for Health Research |
Publisher's version | File Access Level Controlled |
Place of publication | Germany |
Editors | P. Gölitz |
https://acuresearchbank.acu.edu.au/item/8v873/detection-of-localized-hepatocellular-amino-acid-kinetics-by-using-mass-spectrometry-imaging-of-stable-isotopes
Restricted files
Publisher's version
76
total views0
total downloads0
views this month0
downloads this month