Foam cell induction activates ampk but uncouples its regulation of autophagy and lysosomal homeostasis

Journal article

LeBlond, Nicholas D., Nunes, Julia R. C., Smith, Tyler K. T., O’Dwyer, Conor, Robichaud, Sabrina, Gadde, Suresh, Côté, Marceline, Kemp, Bruce E., Ouimet, Mireille and Fullerton, Morgan D.. (2020). Foam cell induction activates ampk but uncouples its regulation of autophagy and lysosomal homeostasis. International Journal of Molecular Sciences. 21(23), pp. 1-21. https://doi.org/10.3390/ijms21239033
AuthorsLeBlond, Nicholas D., Nunes, Julia R. C., Smith, Tyler K. T., O’Dwyer, Conor, Robichaud, Sabrina, Gadde, Suresh, Côté, Marceline, Kemp, Bruce E., Ouimet, Mireille and Fullerton, Morgan D.
Abstract

The dysregulation of macrophage lipid metabolism drives atherosclerosis. AMP-activated protein kinase (AMPK) is a master regulator of cellular energetics and plays essential roles regulating macrophage lipid dynamics. Here, we investigated the consequences of atherogenic lipoprotein-induced foam cell formation on downstream immunometabolic signaling in primary mouse macrophages. A variety of atherogenic low-density lipoproteins (acetylated, oxidized, and aggregated forms) activated AMPK signaling in a manner that was in part due to CD36 and calcium-related signaling. In quiescent macrophages, basal AMPK signaling was crucial for maintaining markers of lysosomal homeostasis as well as levels of key components in the lysosomal expression and regulation network. Moreover, AMPK activation resulted in targeted upregulation of members of this network via transcription factor EB. However, in lipid-induced macrophage foam cells, neither basal AMPK signaling nor its activation affected lysosomal-associated programs. These results suggest that while the sum of AMPK signaling in cultured macrophages may be anti-atherogenic, atherosclerotic input dampens the regulatory capacity of AMPK signaling.

Keywordsmacrophage; AMP-activated protein kinase (AMPK); autophagy; immunometabolism; lysosomal homeostasis; atherosclerosis; foam cell; lipids
Year2020
JournalInternational Journal of Molecular Sciences
Journal citation21 (23), pp. 1-21
PublisherMultidisciplinary Digital Publishing Institute (MDPI AG)
ISSN1422-0067
Digital Object Identifier (DOI)https://doi.org/10.3390/ijms21239033
Scopus EID2-s2.0-85096712263
Open accessPublished as ‘gold’ (paid) open access
Research or scholarlyResearch
Page range1-21
FunderNational Health and Medical Research Council (NHMRC)
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File Access Level
Open
Output statusPublished
Publication dates
Online27 Nov 2020
Publication process dates
Accepted26 Nov 2020
Deposited02 Jun 2021
Grant IDNHMRC/1085460
NHMRC/1078752
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Skeletal muscle AMPK is essential for the maintenance of FNDC5 expression
Lally, James S. V., Ford, Rebecca J., Johar, Jasper, Crane, Justin D., Kemp, Bruce Ernest and Steinberg, Gregory R.. (2015). Skeletal muscle AMPK is essential for the maintenance of FNDC5 expression. Physiological Reports. 3(5), pp. 1 - 9. https://doi.org/10.14814/phy2.12343
Salicylate improves macrophage cholesterol homeostasis via activation of Ampk
Fullerton, Morgan D., Ford, Rebecca J., McGregor, Chelsea P., LeBlond, Nicholas D., Snider, Shayne A., Stypa, Stephanie A., Day, Emily A., Lhoták, Šárka, Schertzer, Jonathan D., Austin, Richard C., Kemp, Bruce Ernest and Steinberg, Gregory R.. (2015). Salicylate improves macrophage cholesterol homeostasis via activation of Ampk. Journal of Lipid Research. 56(5), pp. 1025 - 1033. https://doi.org/10.1194/jlr.M058875
Choreography of AMPK activation
Langerdorf, Christopher G. and Kemp, Bruce Ernest. (2015). Choreography of AMPK activation. Cell Research. 25(1), pp. 5 - 6. https://doi.org/10.1038/cr.2014.163
Skeletal muscle ACC2 S212 phosphorylation is not required for the control of fatty acid oxidation during exercise
O'Neill, Hayley M., Lally, James S., Galic, Sandra, Pulinilkunnil, Thomas, Ford, Rebecca J., Dyck, Jason R. B., Van Denderen, Bryce J,, Kemp, Bruce Ernest and Steinberg, Gregory R.. (2015). Skeletal muscle ACC2 S212 phosphorylation is not required for the control of fatty acid oxidation during exercise. Physiological Reports. 3(7), pp. 1 - 10. https://doi.org/10.14814/phy2.12444
Metformin and salicylate synergistically activate liver AMPK, inhibit lipogenesis and improve insulin sensitivity
Ford, Rebecca, Fullerton, Morgan, Pinkosky, Stephen, Day, Emily, Scott, John, Oakhill, Jonathan, Bujak, Adam, Smith, Brennan, Crane, Justin, Blumer, Regje, Marcinko, Katarina, Kemp, Bruce, Gerstein, Hertzel and Steinberg, Gregory. (2015). Metformin and salicylate synergistically activate liver AMPK, inhibit lipogenesis and improve insulin sensitivity. Biochemical Journal. 468(1), pp. 125 - 132. https://doi.org/10.1042/BJ20150125
High intensity interval training improves liver and adipose tissue insulin sensitivity
Marcinko, Katarina, Sikkema, Sarah R., Samaan, M. Constantine, Kemp, Bruce Ernest, Fullerton, Morgan D. and Steinberg, Gregory R.. (2015). High intensity interval training improves liver and adipose tissue insulin sensitivity. Molecular Metabolism. 4(12), pp. 903 - 915. https://doi.org/10.1016/j.molmet.2015.09.006
Inhibition of AMP-activated protein kinase at the allosteric drug-binding site promotes islet insulin release
Scott, John, Galic, Sandra, Graham, Kate, Foitzik, Richard, Ling, Naomi, Dite, Toby, Issa, Samah, Langendorf, Chris, Weng, Qing, Thomas, Helen, Kay, Thomas, Birnberg, Neal, Steinberg, Gregory, Kemp, Bruce and Oakhill, Jonathan. (2015). Inhibition of AMP-activated protein kinase at the allosteric drug-binding site promotes islet insulin release. Chemistry and Biology. 22(6), pp. 705 - 711. https://doi.org/10.1016/j.chembiol.2015.05.011
AMPK deficiency in cardiac muscle results in dilated cardiomyopathy in the absence of changes in energy metabolism
Sung, Miranda M., Zordoky, Beshay N. M., Bujak, Adam L., Lally, James S. V., Fung, David, Young, Martin E., Horman, Sandrine, Miller, Edward J., Light, Peter E., Kemp, Bruce Ernest, Steinberg, Gregory R. and Dyck, Jason R. B.. (2015). AMPK deficiency in cardiac muscle results in dilated cardiomyopathy in the absence of changes in energy metabolism. Cardiovascular Research. 107(2), pp. 235 - 245. https://doi.org/10.1093/cvr/cvv166
Salicylate activates AMPK and synergizes with metformin to reduce the survival of prostate and lung cancer cells ex vivo through inhibition of de novo lipogenesis
O'Brien, Andrew J., Villani, Linda A., Broadfield, Lindsay A., Houde, Vanessa P., Galic, Sandra, Blandino, Giovanni, Kemp, Bruce Ernest, Tsakiridis, Theodoros, Muti, Paola and Steinberg, Gregory R.. (2015). Salicylate activates AMPK and synergizes with metformin to reduce the survival of prostate and lung cancer cells ex vivo through inhibition of de novo lipogenesis. Biochemical Journal. 469(2), pp. 177 - 187. https://doi.org/10.1042/Bj20150122
The AMPK activator R419 improves exercise capacity and skeletal muscle insulin sensitivity in obese mice
Marcinko, Katarina, Bujak, Adam L., Lally, James S. V., Ford, Rebecca J., Wong, Tammy H., Smith, Brennan K., Kemp, Bruce Ernest, Jenkins, Yonchu, Li, Wei, Kinsella, Todd M., Hitoshi, Yasumichi and Steinberg, Gregory R.. (2015). The AMPK activator R419 improves exercise capacity and skeletal muscle insulin sensitivity in obese mice. Molecular Metabolism. 4(9), pp. 643 - 651. https://doi.org/10.1016/j.molmet.2015.06.002
AMPK activation of muscle autophagy prevents fasting-induced hypoglycemia and myopathy during aging
Bujak, Adam L., Crane, Justin D., Lally, James S., Ford, Rebecca J., Kang, Sally J., Rebalka, Irena A., Green, Alex E., Kemp, Bruce Ernest, Hawke, Thomas J., Schertzer, Jonathan D. and Steinberg, Gregory R.. (2015). AMPK activation of muscle autophagy prevents fasting-induced hypoglycemia and myopathy during aging. Cell Metabolism. 21(6), pp. 883 - 890. https://doi.org/10.1016/j.cmet.2015.05.016
Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging
Crane, Justin D., MacNeil, Lauren G., Lally, James S. V., Ford, Rebecca J., Bujak, Adam L., Brar, Ikdip K., Kemp, Bruce Ernest, Raha, Sandeep, Steinberg, Gregory Robert and Tarnopolsky, Mark A.. (2015). Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging. Aging Cell. 14(4), pp. 625 - 634. https://doi.org/10.1111/acel.12341
Autophosphorylation of CaMKK2 generates autonomous activity that is disrupted by a T85S mutation linked to anxiety and bipolar disorder
Scott, John, Park, Elizabeth, Rodriguiz, Ramona, Oakhill, Jonathan, Issa, Samah, O'Brien, Matthew, Dite, Toby, Langendorf, Christopher, Wetsel, William, Means, Anthony and Kemp, Bruce. (2015). Autophosphorylation of CaMKK2 generates autonomous activity that is disrupted by a T85S mutation linked to anxiety and bipolar disorder. Scientific Reports. 5, pp. 1 - 10. https://doi.org/10.1038/srep14436
SnRK1 from Arabidopsis thaliana is an atypical AMPK
Emanuelle, Shane, Hossain, Mohammed Iqbal, Moller, Isabel E., Pedersen, Henriette L., van de Meene, Allison M. L., Doblin, Monika S., Koay, Ann, Oakhill, Jonathan S., Scott, John W., Willats, William G. T., Kemp, Bruce Ernest, Bacic, Antony, Gooley, Paul R. and Stapleton, David I.. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant Journal. 82(2), pp. 183 - 192. https://doi.org/10.1111/tpj.12813
PPARδ Activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity
Bojic, Lazar, Telford, Dawn, Fullerton, Morgan, Ford, Rebecca, Sutherland, Brian, Edwards, Jane, Sawyez, Cynthia, Gros, Robert, Kemp, Bruce, Steinberg, Gregory and Huff, Murray. (2014). PPARδ Activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity. Journal of Lipid Research. 55(7), pp. 1254 - 1266. https://doi.org/10.1194/jlr.M046037
PPARδ activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity
Bojic, Lazar A., Telford, Dawn E., Fullerton, Morgan D., Ford, Rebecca J., Sutherland, Brian G., Edwards, Jane Y., Sawyez, Cynthia G., Gros, Robert, Kemp, Bruce Ernest, Steinberg, Gregory R. and Huff, Murray W.. (2014). PPARδ activation attenuates hepatic steatosis in Ldlr−/− mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity. Journal of Lipid Research. 55(7), pp. 1254 - 1266. https://doi.org/10.1194/jlr.M046037
Muscle-specific AMPK ß1ß2-null mice display a myopathy due to loss of capillary density in nonpostural muscles
Thomas, Melissa, Wang, David, D'Souza, Donna, Krause, Matthew, Layne, Andrew, Criswell, David, O'Neill, Hayley, Connor, Michael, Anderson, Judy, Kemp, Bruce, Steinberg, Gregory and Hawke, Thomas. (2014). Muscle-specific AMPK ß1ß2-null mice display a myopathy due to loss of capillary density in nonpostural muscles. The FASEB Journal. 28(5), pp. 2098 - 2107. https://doi.org/10.1096/fj.13-238972
Evidence for the role of AMPK in regulating PGC-1 alpha expression and mitochondrial proteins in mouse epididymal adipose tissue
Wan, Zhongxiao, Root-Mccaig, Jared, Castellani, Laura, Kemp, Bruce, Steinberg, Gregory and Wright, David. (2014). Evidence for the role of AMPK in regulating PGC-1 alpha expression and mitochondrial proteins in mouse epididymal adipose tissue. Obesity: a resourch journal. 22(3), pp. 730 - 738. https://doi.org/10.1002/oby.20605
Activation of AMPK reduces the co-transporter activity of NKCC1
Fraser, Scott A., Davies, Matthew R. P., Katerelos, Marina, Gleich, Kurt, Choy, Suet-Wan, Steel, Rohan, Galic, Sandra, Mount, Peter F., Kemp, Bruce Ernest and Power, David A.. (2014). Activation of AMPK reduces the co-transporter activity of NKCC1. Molecular Membrane Biology. 31(2-3), pp. 95 - 102. https://doi.org/10.3109/09687688.2014.902128
Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models
Perera, Nirma, Sheean, Rebecca, Scott, John, Kemp, Bruce, Horne, Malcolm and Turner, Bradley. (2014). Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models. PLoS One (online). 9(3), pp. 1 - 11. https://doi.org/10.1371/journal.pone.0090449
Reduced skeletal muscle AMPK and mitochondrial markers do not promote age-induced insulin resistance
Bujak, Adam, Blumer, Regje, Marcinko, Katarina, Fullerton, Morgan, Kemp, Bruce and Steinberg, Gregory. (2014). Reduced skeletal muscle AMPK and mitochondrial markers do not promote age-induced insulin resistance. Journal of Applied Physiology. 117(2), pp. 171 - 179. https://doi.org/10.1152/japplphysiol.01101.2013
Compensatory regulation of HDAC5 in muscle maintains metabolic adaptive responses and metabolism in response to energetic stress
McGee, Sean, Swinton, Courtney, Morrison, Shona, Gaur, Vidhi, Campbell, Duncan, Jorgensen, Sebastian, Kemp, Bruce, Baar, Keith, Steinberg, Gregory and Hargreaves, Mark. (2014). Compensatory regulation of HDAC5 in muscle maintains metabolic adaptive responses and metabolism in response to energetic stress. The FASEB Journal. 28(8), pp. 3384 - 3395. https://doi.org/10.1096/fj.14-249359
ATP sensitive bi-quinoline activator of the AMP-activated protein kinase
Scott, John, Oakhill, Jonathan, Ling, Naomi, Langendorf, Christopher, Foitzik, Richard, Kemp, Bruce and Issinger, Olaf-Georg. (2014). ATP sensitive bi-quinoline activator of the AMP-activated protein kinase. Biochemical and Biophysical Research Communications. 443(2), pp. 435 - 440. https://doi.org/10.1016/j.bbrc.2013.11.130
Enhanced activation of cellular AMPK by dual-small molecule treatment: AICAR and A769662
Ducommun, Serge, Ford, Rebecca J., Bultot, Laurent, Deak, Maria, Bertrand, Luc, Kemp, Bruce Ernest, Steinberg, Gregory R. and Sakamoto, Kei. (2014). Enhanced activation of cellular AMPK by dual-small molecule treatment: AICAR and A769662. American Journal of Physiology - Endocrinology and Metabolism. 306(6), pp. 688 - 696. https://doi.org/10.1152/ajpendo.00672.2013
Small molecule drug A-769662 and AMP synergistically activate naive AMPK independent of upstream kinase signaling
Scott, John, Ling, Naomi, Issa, Samah, Dite, Toby, O'Brien, Matthew, Chen, Zhi-Ping, Galic, Sandra, Langendorf, Christopher, Steinberg, Gregory, Kemp, Bruce and Oakhill, Jonathan. (2014). Small molecule drug A-769662 and AMP synergistically activate naive AMPK independent of upstream kinase signaling. Chemistry and Biology. 21(5), pp. 619 - 627. https://doi.org/10.1016/j.chembiol.2014.03.006
Novel mechanisms of Na+ retention in obesity: Phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK
Davies, Matthew R. P., Fraser, Scott A., Galic, Sandra, Choy, Suet-Wan, Katerelos, Marina, Gleich, Kurt, Kemp, Bruce Ernest, Mount, Peter F. and Power, David A.. (2014). Novel mechanisms of Na+ retention in obesity: Phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK. American Journal of Physiology - Renal Physiology. 307(1), pp. 96 - 106. https://doi.org/10.1152/ajprenal.00524.2013
Mechanism of action of compound-13 : An a1-selective small molecule activator of AMPK
Hunter, Roger, Foretz, Marc, Bultot, Laurent, Fullerton, Morgan, Deak, Maria, Ross, Fiona, Hawley, Simon, Shpiro, Natalia, Viollet, Benoit, Barron, Denis, Kemp, Bruce, Steinberg, Gregory, Hardie, David Grahame and Sakamoto, Kei. (2014). Mechanism of action of compound-13 : An a1-selective small molecule activator of AMPK. Chemistry and Biology. 21(7), pp. 866 - 879. https://doi.org/10.1016/j.chembiol.2014.05.014
Single phosphorylation sites in Acc1 and Acc2 regulate lipid homeostasis and the insulin-sensitizing effects of metformin
Fullerton, Morgan, Galic, Sandra, Marcinko, Katarina, Sikkema, Sarah, Pulinilkunnil, Thomas, Chen, Zhi-Ping, O'Neill, Hayley, Ford, Rebecca, Palanivel, Rengasamy, O'Brien, Matthew, Hardie, D. Grahame, Macaulay, Lance, Schertzer, Jonathan, Dyck, Jason, van Denderen, Bryce, Kemp, Bruce and Steinberg, Gregory. (2013). Single phosphorylation sites in Acc1 and Acc2 regulate lipid homeostasis and the insulin-sensitizing effects of metformin. Nature Medicine. 19(12), pp. 1649 - 1654. https://doi.org/10.1038/nm.3372
AMPK phosphorylation of ACC2 is required for skeletal muscle fatty acid oxidation and insulin sensitivity in mice
O’Neill, Hayley M., Lally, James S., Galic, Sandra, Thomas, Melissa, Azizi, Paymon D., Fullerton, Morgan D., Smith, Brennan K., Pulinilkunnil, Thomas, Chen, Zhiping, Constantine Samaan, M., Jorgensen, Sebastian B., Dyck, Jason R. B., Holloway, Graham P., Hawke, Thomas J., van Denderen, Bryce J., Kemp, Bruce E. and Steinberg, Gregory R.. (2012). AMPK phosphorylation of ACC2 is required for skeletal muscle fatty acid oxidation and insulin sensitivity in mice. Diabetologia. 57(8), pp. 1693 - 1702. https://doi.org/10.1007/s00125-014-3273-1
AMPK-dependent inhibitory phosphorylation of ACC is not essential for maintaining myocardial fatty acid oxidation
Zordoky, Beshay N.M., Nagendran, Jeevan, Pulinilkunnil, Thomas, Kienesberger, Petra C., Masson, Grant, Waller, Terri J., Kemp, Bruce E., Steinberg, Gregory R. and Dyck, Jason R. B.. (2012). AMPK-dependent inhibitory phosphorylation of ACC is not essential for maintaining myocardial fatty acid oxidation. Circulation Research. 115(5), pp. 518 - 524. https://doi.org/10.1161/CIRCRESAHA.115.304538
The ancient drug salicylate directly activates AMP-activated protein kinase
Hawley, Simon, Fullerton, Morgan, Ross, Fiona, Schertzer, Jonathan, Chevtzoff, Cyrille, Walker, Katherine, Peggie, Mark, Zibrova, Darya, Green, Kevin, Mustard, Kirsty, Kemp, Bruce, Sakamoto, Kei, Steinberg, Gregory and Hardie, D. Grahame. (2012). The ancient drug salicylate directly activates AMP-activated protein kinase. Science. 336(6083), pp. 918 - 922. https://doi.org/10.1126/science.1215327
Pro-GRP-derived peptides are expressed in colorectal cancer cells and tumors and are biologically active in vivo
Patel, Oneel, Clyde, Daniel, Chang, Mike, Nordlund, Marianne, Steel, Rohan, Kemp, Bruce, Pritchard, D. Mark, Shulkes, Arthur and Baldwin, Graham. (2012). Pro-GRP-derived peptides are expressed in colorectal cancer cells and tumors and are biologically active in vivo. Endocrinology. 153(3), pp. 1082 - 1092. https://doi.org/10.1210/en.2011-1875
The outcome of renal ischemia-reperfusion injury is unchanged in AMPK-ß1 deficient mice
Mount, Peter, Gleich, Kurt, Tam, Shanna, Fraser, Scott, Choy, Suet-Wan, Dwyer, Karen, Lu, Bo, Van Denderen, Bryce, Fingerle-Rowson, Gunter, Bucala, Richard, Kemp, Bruce and Power, David. (2012). The outcome of renal ischemia-reperfusion injury is unchanged in AMPK-ß1 deficient mice. PLoS One (online). 7(1), pp. 1 - 10. https://doi.org/10.1371/journal.pone.0029887
Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase
Alesultan, Ioana, Foller, Michael, Sopjani, Mentor, Dermaku-Sopjani, Miribane, Zelenak, Christine, Frohlich, Henning, Velic, Ana, Fraser, Scott, Kemp, Bruce, Seebohm, Guiscard, Volkl, Harald and Lang, Florian. (2011). Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase. Molecular Membrane Biology. 28(2), pp. 79 - 89. https://doi.org/10.3109/09687688.2010.520037
AMP-activated protein kinase (AMPK) ß1ß2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise
O'Neill, Hayley M., Maarbjerg, Stine J., Crane, Justin D., Jeppesen, Jacob, Jorgensen, Sebastian B., Schertzer, Jonathan D., Shyroka, Olga, Kiens, Bente, Van Denderen, Bryce J., Tarnopolsky, Mark A., Kemp, Bruce Ernest, Richter, Erik A. and Steinberg, Gregory R.. (2011). AMP-activated protein kinase (AMPK) ß1ß2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Proceedings of the National Academy of Sciences of USA. 108(38), pp. 16092 - 16097. https://doi.org/10.1073/pnas.1105062108
Ca 2+/calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation
Green, Michelle, Scott, John, Steel, Rohan, Oakhill, Jonathan, Kemp, Bruce and Means, Anthony. (2011). Ca 2+/calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation. Journal of Biological Chemistry. 286(32), pp. 28066 - 28079. https://doi.org/10.1074/jbc.M111.251504
Hematopoietic AMPK beta1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity
Galic, Sandra, Fullerton, Morgan, Schertzer, Jonathan, Sikkema, Sarah, Marcinko, Katarina, Walkley, Carl, Izon, David, Honeyman, Jane, Chen, Zhi-Ping, Van Denderen, Bryce, Kemp, Bruce and Steinberg, Gregory. (2011). Hematopoietic AMPK beta1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. Journal of Clinical Investigation. 121(12), pp. 4903 - 4915. https://doi.org/10.1172/JCI58577
Hematopoietic AMPK Beta ß1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity
Galic, Sandra, Fullerton, Morgan D., Schertzer, Jonathan D., Sikkema, Sarah, Marcinko, Katarina, Walkley, Carl R., Izon, David, Honeyman, Jane, Chen, Zhi-Ping, van Denderen, Bryce J., Kemp, Bruce Ernest and Steinberg, Gregory R.. (2011). Hematopoietic AMPK Beta ß1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. Journal of Clinical Investigation. 121(12), pp. 4903 - 4915. https://doi.org/10.1172/JCI58577
Inhibition of Kir2.1 (KCNJ2) by the AMP-activated protein kinase
Alesutan, Ioana, Munoz, Carlos, Sopjani, Mentor, Dermaku-Sopjani, Miribane, Michael, Diana, Fraser, Scott, Kemp, Bruce, Seebohm, Guiscard, Foller, Michael and Lang, Florian. (2011). Inhibition of Kir2.1 (KCNJ2) by the AMP-activated protein kinase. Biochemical and Biophysical Research Communications. 408(4), pp. 505 - 510. https://doi.org/10.1016/j.bbrc.2011.04.015
Inhibition of Connexin 26 by the AMP-activated protein kinase
Alesutan, Ioana, Sopjani, Mentor, Munoz, Carlos, Fraser, Scott, Kemp, Bruce, Foller, Michael and Lang, Florian. (2011). Inhibition of Connexin 26 by the AMP-activated protein kinase. Journal of Membrane Biology: an international journal for studies on the structure, function and genesis of biomembranes. 240(3), pp. 151 - 158. https://doi.org/10.1007/s00232-011-9353-y
AMPK is a direct adenylate charge-regulated protein kinase
Oakhill, Jonathan S., Steel, Rohan, Chen, Zhi-Ping, Scott, John W., Ling, Naomi, Tam, Shanna and Kemp, Bruce Ernest. (2011). AMPK is a direct adenylate charge-regulated protein kinase. Science. 332(6036), pp. 1433 - 1435. https://doi.org/10.1126/science.1200094
AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1
Fraser, Scott A., Choy, Suet-Wan, Pastor-Soler, Núria M., Li, Hui, Davies, Matthew R. P., Cook, Natasha, Katerelos, Marina, Mount, Peter F., Gleich, Kurt, McRae, Jennifer L., Dwyer, Karen M., van Denderen, Bryce J. W., Hallows, Kenneth R., Kemp, Bruce E. and Power, David A.. (2010). AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1. American Journal of Physiology - Renal Physiology. 305(5), pp. F679 - F690. https://doi.org/10.1152/ajprenal.00407.2012
Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated with whole-body insulin resistance in rats bred for low intrinsic exercise capacity
Lessard, Sarah J., Rivas, Donato A., Chen, Zhu-Ping, van Denderen, Bryce J., Watt, Matthew J., Koch, Lauren G., Britton, Steven L., Kemp, Bruce Ernest and Hawley, John Alan. (2009). Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated with whole-body insulin resistance in rats bred for low intrinsic exercise capacity. Endocrinology. 150(11), pp. 4883 - 4891. https://doi.org/10.1210/en.2009-0158
Fat adaptation followed by carbohydrate restoration increases AMPK activity in skeletal muscle from trained humans
Yeo, Wee Kian, Lessard, Sarah J., Chen, Zhi-Ping, Garnham, Andrew P., Burke, Louise, Rivas, Donato A., Kemp, Bruce Ernest and Hawley, John Alan. (2008). Fat adaptation followed by carbohydrate restoration increases AMPK activity in skeletal muscle from trained humans. Journal of Applied Physiology. 105(5), pp. 1519 - 1526. https://doi.org/10.1152/japplphysiol.90540.2008
Activation of AMPK reduces the co-transporter activity of NKCC1
Fraser, Scott A., Davies, Matthew, Katerelos, Marina, Gleich, Kurt, Choy, Suet-Wan, Steel, Rohan, Galic, Sandra, Mount, Peter F., Kemp, Bruce E. and Power, David A.. (1999). Activation of AMPK reduces the co-transporter activity of NKCC1. Molecular Membrane Biology. 31(2-3), pp. 95 - 102. https://doi.org/10.3109/09687688.2014.902128
Myosin light chain kinases
Kemp, Bruce E. and Stull, James T.. (1990). Myosin light chain kinases. In In B. E. Kemp (Ed.). Peptides and Protein Phosphorylation pp. 115 - 133 CRC Press. https://doi.org/10.1201/9781351075442