Collagen immunoassay as a method to optimise surface functionalisation

Journal article

Stynes, Gil, Kiroff, George, Morrison, Wayne and Kirkland, Mark. (2017). Collagen immunoassay as a method to optimise surface functionalisation. Plasma Processes and Polymers. 14(9), p. 1600213. https://doi.org/10.1002/ppap.201600213
AuthorsStynes, Gil, Kiroff, George, Morrison, Wayne and Kirkland, Mark
Abstract

Traditional methods of assessing surface functionalisation, including spectroscopy and chemical labelling, often involve significant error and conjecture about bonds. Proteins that improve cell attachment have specific pKa's and optimum binding requirements that may differ from the conditions required for chemical labelling. The utility of collagen ELISA to optimise acetaldehyde glow discharge polymerisation reactor parameters was tested. Accurate stepwise increases in collagen conjugation strength were demonstrated by incubating specimens in 8 M urea for 5–8 days followed by ELISA to test for residual surface collagen. Surface modifications also were assessed by XPS. The results indicated that ELISA after bond-stressing with urea may suffice for optimising surface functionalisation and that traditional methods of analysis may be superfluous if protein conjugation is the aim of functionalisation.

Keywordscollagen; functionalisation of polymers; glow discharge polymerisation; immobilisation of molecules; surface modification
Year2017
JournalPlasma Processes and Polymers
Journal citation14 (9), p. 1600213
PublisherWiley - V C H Verlag GmbH & Co. KGaA
ISSN1612-8869
Digital Object Identifier (DOI)https://doi.org/10.1002/ppap.201600213
Scopus EID2-s2.0-85013035498
Research or scholarlyResearch
Page range1-9
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Output statusPublished
Publication dates
Online07 Feb 2017
Publication process dates
Accepted17 Jan 2017
Deposited10 Jun 2021
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