Collagen immunoassay as a method to optimise surface functionalisation
Journal article
Stynes, Gil, Kiroff, George, Morrison, Wayne and Kirkland, Mark. (2017). Collagen immunoassay as a method to optimise surface functionalisation. Plasma Processes and Polymers. 14(9), p. 1600213. https://doi.org/10.1002/ppap.201600213
Authors | Stynes, Gil, Kiroff, George, Morrison, Wayne and Kirkland, Mark |
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Abstract | Traditional methods of assessing surface functionalisation, including spectroscopy and chemical labelling, often involve significant error and conjecture about bonds. Proteins that improve cell attachment have specific pKa's and optimum binding requirements that may differ from the conditions required for chemical labelling. The utility of collagen ELISA to optimise acetaldehyde glow discharge polymerisation reactor parameters was tested. Accurate stepwise increases in collagen conjugation strength were demonstrated by incubating specimens in 8 M urea for 5–8 days followed by ELISA to test for residual surface collagen. Surface modifications also were assessed by XPS. The results indicated that ELISA after bond-stressing with urea may suffice for optimising surface functionalisation and that traditional methods of analysis may be superfluous if protein conjugation is the aim of functionalisation. |
Keywords | collagen; functionalisation of polymers; glow discharge polymerisation; immobilisation of molecules; surface modification |
Year | 2017 |
Journal | Plasma Processes and Polymers |
Journal citation | 14 (9), p. 1600213 |
Publisher | Wiley - V C H Verlag GmbH & Co. KGaA |
ISSN | 1612-8869 |
Digital Object Identifier (DOI) | https://doi.org/10.1002/ppap.201600213 |
Scopus EID | 2-s2.0-85013035498 |
Research or scholarly | Research |
Page range | 1-9 |
Publisher's version | License All rights reserved File Access Level Controlled |
Output status | Published |
Publication dates | |
Online | 07 Feb 2017 |
Publication process dates | |
Accepted | 17 Jan 2017 |
Deposited | 10 Jun 2021 |
https://acuresearchbank.acu.edu.au/item/8w38x/collagen-immunoassay-as-a-method-to-optimise-surface-functionalisation
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