Vascular pedicle and microchannels : Simple methods toward effective in vivo vascularization of 3D scaffolds

Journal article


Rnjak-Kovacina, Jelena, Gerrand, Yi-wen, Wray, Lindsay S., Tan, Beryl, Joukhdar, Habib, Kaplan, David L., Morrison, Wayne A. and Mitchell, Geraldine M.. (2019) Vascular pedicle and microchannels : Simple methods toward effective in vivo vascularization of 3D scaffolds. Advanced Healthcare Materials. 8(24), p. 1901106. https://doi.org/10.1002/adhm.201901106
AuthorsRnjak-Kovacina, Jelena, Gerrand, Yi-wen, Wray, Lindsay S., Tan, Beryl, Joukhdar, Habib, Kaplan, David L., Morrison, Wayne A. and Mitchell, Geraldine M.
Abstract

Poor vascularization remains a key limiting factor in translating advances in tissue engineering to clinical applications. Vascular pedicles (large arteries and veins) isolated in plastic chambers are known to sprout an extensive capillary network. This study examined the effect vascular pedicles and scaffold architecture have on vascularization and tissue integration of implanted silk scaffolds. Porous silk scaffolds with or without microchannels are manufactured to support implantation of a central vascular pedicle, without a chamber, implanted in the groin of Sprague Dawley rats, and assessed morphologically and morphometrically at 2 and 6 weeks. At both time points, blood vessels, connective tissue, and an inflammatory response infiltrate all scaffold pores externally, and centrally when a vascular pedicle is implanted. At week 2, vascular pedicles significantly increase the degree of scaffold tissue infiltration, and both the pedicle and the scaffold microchannels significantly increase vascular volume and vascular density. Interestingly, microchannels contribute to increased scaffold vascularity without affecting overall tissue infiltration, suggesting a direct effect of biomaterial architecture on vascularization. The inclusion of pedicles and microchannels are simple and effective proangiogenic techniques for engineering thick tissue constructs as both increase the speed of construct vascularization in the early weeks post in vivo implantation.

Keywordsangiogenesis; microchannels; silk biomaterials; vascular pedicles; vascularization
Year2019
JournalAdvanced Healthcare Materials
Journal citation8 (24), p. 1901106
PublisherWiley - V C H Verlag GmbH & Co. KGaA
ISSN2192-2640
Digital Object Identifier (DOI)https://doi.org/10.1002/adhm.201901106
Scopus EID2-s2.0-85075004317
Research or scholarlyResearch
Page range1-12
Publisher's version
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All rights reserved
File Access Level
Controlled
Output statusPublished
Publication dates
Online12 Nov 2019
Publication process dates
Deposited16 Jun 2021
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