Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis : In vitro and in vivo assessment

Journal article

Tran, Phong A., O'Brien-Simpson, Neil, Palmer, Jason A., Bock, Nathalie, Reynolds, Eric C., Webster, Thomas J., Deva, Anand, Morrison, Wayne A. and O'Connor, Andrea J.. (2019). Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis : In vitro and in vivo assessment. International Journal of Nanomedicine. 14, pp. 4613-4624. https://doi.org/10.2147/IJN.S197737
AuthorsTran, Phong A., O'Brien-Simpson, Neil, Palmer, Jason A., Bock, Nathalie, Reynolds, Eric C., Webster, Thomas J., Deva, Anand, Morrison, Wayne A. and O'Connor, Andrea J.
Abstract

Background: Bacterial infection is a common and serious complication in orthopedic implants following traumatic injury, which is often associated with extensive soft tissue damage and contaminated wounds. Multidrug-resistant bacteria have been found in these infected wounds, especially in patients who have multi trauma and prolonged stay in intensive care units.Purpose: The objective of this study was to develop a coating on orthopedic implants that is effective against drug-resistant bacteria.

Methods and results: We applied nanoparticles (30-70nm) of the trace element selenium (Se) as a coating through surface-induced nucleation-deposition on titanium implants and investigated the antimicrobial activity against drug resistant bacteria including Methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus epidermidis (MRSE) in vitro and in an infected femur model in rats.The nanoparticles were shown in vitro to have antimicrobial activity at concentrations as low as 0.5ppm. The nanoparticle coatings strongly inhibited biofilm formation on the implants and reduced the number of viable bacteria in the surrounding tissue following inoculation of implants with biofilm forming doses of bacteria.

Conclusion: This study shows a proof of concept for a selenium nanoparticle coatings as a potential anti-infective barrier for orthopedic medical devices in the setting of contamination with multi-resistant bacteria. It also represents one of the few (if only) in vivo assessment of selenium nanoparticle coatings on reducing antibiotic-resistant orthopedic implant infections.

Keywordsorthopedic; implants; antimicrobial; biofilm; selenium; nanoparticles
Year2019
JournalInternational Journal of Nanomedicine
Journal citation14, pp. 4613-4624
PublisherDove Medical Press Ltd.
ISSN1176-9114
Digital Object Identifier (DOI)https://doi.org/10.2147/IJN.S197737
Scopus EID2-s2.0-85069924585
Open accessPublished as ‘gold’ (paid) open access
Research or scholarlyResearch
Page range4613-4624
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online01 Jul 2019
Publication process dates
Accepted16 Apr 2019
Deposited12 Jul 2021
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