Melatonin protects human adipose-derived stem cells from oxidative stress and cell death

Journal article

Tan, Shaun S., Han, Xiaolian, Sivakumaran, Priyadharshini, Lim, Shiang Y. and Morrison, Wayne A.. (2016). Melatonin protects human adipose-derived stem cells from oxidative stress and cell death. Archives of Plastic Surgery. 43(3), pp. 237-241. https://doi.org/10.5999/aps.2016.43.3.237
AuthorsTan, Shaun S., Han, Xiaolian, Sivakumaran, Priyadharshini, Lim, Shiang Y. and Morrison, Wayne A.
Abstract

Background
Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs.

Methods
We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours. Cell viability and apoptosis were determined with propidium iodide and Hoechst 33342 staining under fluorescence microscopy.

Results
Hydrogen peroxide (1–2.5 mM) treatment resulted in an incremental increase in cell death. 2 mM hydrogen peroxide was thereafter selected as the dose for co-treatment with melatonin. Melatonin alone had no adverse effects on ASCs. Co-treatment of ASCs with melatonin in the presence of hydrogen peroxide protected ASCs from cell death in a dose-dependent manner, and afforded maximal protection at 100 µM (n=4, one-way analysis of variance P<0.001). Melatonin co-treated ASCs displayed significantly fewer apoptotic cells, as demonstrated by condensed and fragmented nuclei under fluorescence microscopy.

Conclusions
Melatonin possesses cytoprotective properties against oxidative stress in human ASCs and might be a useful adjunct in fat grafting and cell-assisted lipotransfer.

Keywordsmelatonin; hydrogen peroxide; mesenchymal stem cell; cytoprotection
Year2016
JournalArchives of Plastic Surgery
Journal citation43 (3), pp. 237-241
PublisherTaehan Songhyong Oekwa Hakhoe
ISSN2234-6163
2234-6171
Digital Object Identifier (DOI)https://doi.org/10.5999/aps.2016.43.3.237
PubMed ID27218020
Scopus EID2-s2.0-84969690084
PubMed Central IDPMC4876151
Open accessPublished as ‘gold’ (paid) open access
Research or scholarlyResearch
Page range237-241
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online18 May 2016
Publication process dates
Accepted12 Apr 2016
Deposited18 Nov 2021
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