Myofibre hypertrophy in the absence of changes to satellite cell content following concurrent exercise training in young healthy men
Journal article
Shamim, Baubak, Camera, Donny M. and Whitfield, Jamie. (2021). Myofibre hypertrophy in the absence of changes to satellite cell content following concurrent exercise training in young healthy men. Frontiers in Physiology. 12, p. Article 625044. https://doi.org/10.3389/fphys.2021.625044
Authors | Shamim, Baubak, Camera, Donny M. and Whitfield, Jamie |
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Abstract | Concurrent exercise training has been suggested to create an ‘interference effect,’ attenuating resistance training-based skeletal muscle adaptations, including myofibre hypertrophy. Satellite cells support myofibre hypertrophy and are influenced by exercise mode. To determine whether satellite cells contribute to the ‘interference effect’ changes in satellite cell and myonuclear content were assessed following a period of training in 32 recreationally active males (age: 25 ± 5 year; body mass index: 24 ± 3 kg⋅m–2; mean ± SD) who undertook 12-week of either isolated (3 d⋅w–1) resistance (RES; n = 10), endurance (END; n = 10), or alternate day (6 d⋅w–1) concurrent (CET, n = 12) training. Skeletal muscle biopsies were obtained pre-intervention and after 2, 8, and 12 weeks of training to determine fibre type-specific cross-sectional area (CSA), satellite cell content (Pax7+DAPI+), and myonuclei (DAPI+) using immunofluorescence microscopy. After 12 weeks, myofibre CSA increased in all training conditions in type II (P = 0.0149) and mixed fibres (P = 0.0102), with no difference between conditions. Satellite cell content remained unchanged after training in both type I and type II fibres. Significant correlations were observed between increases in fibre type-specific myonuclear content and CSA of Type I (r = 0.63, P < 0.0001), Type II (r = 0.69, P < 0.0001), and mixed fibres (r = 0.72, P < 0.0001). Resistance, endurance, and concurrent training induce similar myofibre hypertrophy in the absence of satellite cell and myonuclear pool expansion. These findings suggest that myonuclear accretion via satellite cell fusion is positively correlated with hypertrophy after 12 weeks of concurrent training, and that individuals with more myonuclear content displayed greater myofibre hypertrophy. |
Keywords | concurrent exercise; resistance exercise; endurance exercise; skeletal muscle; satellite cells |
Year | 2021 |
Journal | Frontiers in Physiology |
Journal citation | 12, p. Article 625044 |
Publisher | Frontiers Research Foundation |
ISSN | 1664-042X |
Digital Object Identifier (DOI) | https://doi.org/10.3389/fphys.2021.625044 |
PubMed ID | 34149439 |
Scopus EID | 2-s2.0-85108201870 |
PubMed Central ID | PMC8213074 |
Open access | Published as ‘gold’ (paid) open access |
Research or scholarly | Research |
Page range | 1-13 |
Funder | Australian Catholic University (ACU) |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 04 Jun 2021 |
Publication process dates | |
Accepted | 11 May 2021 |
Deposited | 18 Nov 2021 |
Grant ID | ACU/36331 |
https://acuresearchbank.acu.edu.au/item/8x180/myofibre-hypertrophy-in-the-absence-of-changes-to-satellite-cell-content-following-concurrent-exercise-training-in-young-healthy-men
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Publisher's version
OA_Shamim_2021_Myofibre_hypertrophy_in_the_absence_of.pdf | |
License: CC BY 4.0 | |
File access level: Open |
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