Enterovirus-specific anti-peptide antibodies

Book chapter


Poh, Chit Laa, Kirk, Katherine, Chua, Hui Na and Grollo, Lara. (2015). Enterovirus-specific anti-peptide antibodies. In In Houen, Gunnar (Ed.). Peptide antibodies methods and protocols pp. 341-350 Humana Press. https://doi.org/10.1007/978-1-4939-2999-3_29
AuthorsPoh, Chit Laa, Kirk, Katherine, Chua, Hui Na and Grollo, Lara
EditorsHouen, Gunnar
Abstract

Enterovirus 71 (EV-71) is the main causative agent of hand, foot, and mouth disease (HFMD) which is generally regarded as a mild childhood disease. In recent years, EV71 has emerged as a significant pathogen capable of causing high mortalities and severe neurological complications in large outbreaks in Asia. A formalin-inactivated EV71 whole virus vaccine has completed phase III trial in China but is currently unavailable clinically. The high cost of manufacturing and supply problems may limit practical implementations in developing countries. Synthetic peptides representing the native primary structure of the viral immunogen which is able to elicit neutralizing antibodies can be made readily and is cost effective. However, it is necessary to conjugate short synthetic peptides to carrier proteins to enhance their immunogenicity. This review describes the production of cross-neutralizing anti-peptide antibodies in response to immunization with synthetic peptides selected from in silico analysis, generation of B-cell epitopes of EV71 conjugated to a promiscuous T-cell epitope from Poliovirus, and evaluation of the neutralizing activities of the anti-peptide antibodies. Besides neutralizing EV71 in vitro, the neutralizing antibodies were cross-reactive against several Enteroviruses including CVA16, CVB4, CVB6, and ECHO13.

Keywordsenterovirus 71; enterovirus-specific anti-peptide antibodies; cross-reactive neutralizing antibody epitopes; in silico analysis of B-cell epitopes
Page range341-350
Year2015
Book titlePeptide antibodies methods and protocols
PublisherHumana Press
Place of publicationNew York, NY
Heidelberg, Germany
Dordrecht, The Netherlands
London, United Kingdom
SeriesMethods in molecular biology
ISBN9781493929993
9781493929986
9781493929986
ISSN1064-3745
1940-6029
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-4939-2999-3_29
Scopus EID2-s2.0-84942908645
Research or scholarlyResearch
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All rights reserved
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Controlled
Output statusPublished
Publication process dates
Deposited10 Dec 2021
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