Dietary nitrate does not reduce oxygen cost of exercise or improve muscle mitochondrial function in patients with mitochondrial myopathy
Journal article
Nabben, Miranda, Schmitz, Joep P. J., Ciapaite, Jolita, le Clercq, Carlijn M. P., van Riel, Natal A., Haak, Harm R., Nicolay, Klaas, de Coo, Irenaeus F. M., Smeets, Hubert, Praet, Stephan F., van Loon, Lucas J. and Prompers, Jeanine J.. (2017). Dietary nitrate does not reduce oxygen cost of exercise or improve muscle mitochondrial function in patients with mitochondrial myopathy. American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 312(5), pp. R689-R701. https://doi.org/10.1152/ajpregu.00264.2016
Authors | Nabben, Miranda, Schmitz, Joep P. J., Ciapaite, Jolita, le Clercq, Carlijn M. P., van Riel, Natal A., Haak, Harm R., Nicolay, Klaas, de Coo, Irenaeus F. M., Smeets, Hubert, Praet, Stephan F., van Loon, Lucas J. and Prompers, Jeanine J. |
---|---|
Abstract | Muscle weakness and exercise intolerance negatively affect the quality of life of patients with mitochondrial myopathy. Short-term dietary nitrate supplementation has been shown to improve exercise performance and reduce oxygen cost of exercise in healthy humans and trained athletes. We investigated whether 1 wk of dietary inorganic nitrate supplementation decreases the oxygen cost of exercise and improves mitochondrial function in patients with mitochondrial myopathy. Ten patients with mitochondrial myopathy (40 ± 5 yr, maximal whole body oxygen uptake = 21.2 ± 3.2 ml·min−1·kg body wt−1, maximal work load = 122 ± 26 W) received 8.5 mg·kg body wt−1·day−1 inorganic nitrate (~7 mmol) for 8 days. Whole body oxygen consumption at 50% of the maximal work load, in vivo skeletal muscle oxidative capacity (evaluated from postexercise phosphocreatine recovery using 31P-magnetic resonance spectroscopy), and ex vivo mitochondrial oxidative capacity in permeabilized skinned muscle fibers (measured with high-resolution respirometry) were determined before and after nitrate supplementation. Despite a sixfold increase in plasma nitrate levels, nitrate supplementation did not affect whole body oxygen cost during submaximal exercise. Additionally, no beneficial effects of nitrate were found on in vivo or ex vivo muscle mitochondrial oxidative capacity. This is the first time that the therapeutic potential of dietary nitrate for patients with mitochondrial myopathy was evaluated. We conclude that 1 wk of dietary nitrate supplementation does not reduce oxygen cost of exercise or improve mitochondrial function in the group of patients tested. |
Keywords | mitochondrial myopathy; nitrate; 31P-magnetic resonance spectroscopy; V̇o2peak; permeabilized muscle fibers |
Year | 2017 |
Journal | American Journal of Physiology - Regulatory Integrative and Comparative Physiology |
Journal citation | 312 (5), pp. R689-R701 |
Publisher | American Physiological Society |
ISSN | 1522-1490 |
Digital Object Identifier (DOI) | https://doi.org/10.1152/ajpregu.00264.2016 |
Scopus EID | 2-s2.0-85018284486 |
Research or scholarly | Research |
Page range | R689-R701 |
Publisher's version | License All rights reserved File Access Level Controlled |
Output status | Published |
Publication dates | |
Online | 01 May 2017 |
01 May 2017 | |
Publication process dates | |
Accepted | 06 Feb 2017 |
Deposited | 25 Feb 2022 |
https://acuresearchbank.acu.edu.au/item/8x5zx/dietary-nitrate-does-not-reduce-oxygen-cost-of-exercise-or-improve-muscle-mitochondrial-function-in-patients-with-mitochondrial-myopathy
Restricted files
Publisher's version
59
total views0
total downloads0
views this month0
downloads this month