Comprehensive assessment of post-prandial protein handling by the application of intrinsically labelled protein in vivo in human subjects
Journal article
Trommelen, Jorn, Holwerda, Andrew M., Pinckaers, Philippe J. M. and van Loon, Lucas J. C.. (2021). Comprehensive assessment of post-prandial protein handling by the application of intrinsically labelled protein in vivo in human subjects. Proceedings of the Nutrition Society. 80(2), pp. 221-229. https://doi.org/10.1017/S0029665120008034
Authors | Trommelen, Jorn, Holwerda, Andrew M., Pinckaers, Philippe J. M. and van Loon, Lucas J. C. |
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Abstract | All human tissues are in a constant state of remodelling, regulated by the balance between tissue protein synthesis and breakdown rates. It has been well-established that protein ingestion stimulates skeletal muscle and whole-body protein synthesis. Stable isotope-labelled amino acid methodologies are commonly applied to assess the various aspects of protein metabolism in vivo in human subjects. However, to achieve a more comprehensive assessment of post-prandial protein handling in vivo in human subjects, intravenous stable isotope-labelled amino acid infusions can be combined with the ingestion of intrinsically labelled protein and the collection of blood and muscle tissue samples. The combined application of ingesting intrinsically labelled protein with continuous intravenous stable isotope-labelled amino acid infusion allows the simultaneous assessment of protein digestion and amino acid absorption kinetics (e.g. release of dietary protein-derived amino acids into the circulation), whole-body protein metabolism (whole-body protein synthesis, breakdown and oxidation rates and net protein balance) and skeletal muscle metabolism (muscle protein fractional synthesis rates and dietary protein-derived amino acid incorporation into muscle protein). The purpose of this review is to provide an overview of the various aspects of post-prandial protein handling and metabolism with a focus on insights obtained from studies that have applied intrinsically labelled protein under a variety of conditions in different populations. |
Keywords | digestibility; anabolism; hypertrophy; protein quality |
Year | 2021 |
Journal | Proceedings of the Nutrition Society |
Journal citation | 80 (2), pp. 221-229 |
Publisher | Cambridge University Press |
ISSN | 0029-6651 |
Digital Object Identifier (DOI) | https://doi.org/10.1017/S0029665120008034 |
Scopus EID | 2-s2.0-85099919893 |
Open access | Published as ‘gold’ (paid) open access |
Research or scholarly | Research |
Page range | 221-229 |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 25 Jan 2021 |
Publication process dates | |
Deposited | 25 Mar 2022 |
https://acuresearchbank.acu.edu.au/item/8x9vq/comprehensive-assessment-of-post-prandial-protein-handling-by-the-application-of-intrinsically-labelled-protein-in-vivo-in-human-subjects
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Publisher's version
OA_Trommelen_2021_Comprehensive_assessment_of_post-prandial_protein.pdf | |
License: CC BY 4.0 | |
File access level: Open |
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