Metabolomics and lipidomics : Expanding the molecular landscape of exercise biology

Journal article

Belhaj, Mehdi R., Lawler, Nathan G. and Hoffman, Nolan J.. (2021). Metabolomics and lipidomics : Expanding the molecular landscape of exercise biology. Metabolites. 11(151), p. Article 151. https://doi.org/10.3390/metabo11030151
AuthorsBelhaj, Mehdi R., Lawler, Nathan G. and Hoffman, Nolan J.
Abstract

Dynamic changes in circulating and tissue metabolites and lipids occur in response to exercise-induced cellular and whole-body energy demands to maintain metabolic homeostasis. The metabolome and lipidome in a given biological system provides a molecular snapshot of these rapid and complex metabolic perturbations. The application of metabolomics and lipidomics to map the metabolic responses to an acute bout of aerobic/endurance or resistance exercise has dramatically expanded over the past decade thanks to major analytical advancements, with most exercise-related studies to date focused on analyzing human biofluids and tissues. Experimental and analytical considerations, as well as complementary studies using animal model systems, are warranted to help overcome challenges associated with large human interindividual variability and decipher the breadth of molecular mechanisms underlying the metabolic health-promoting effects of exercise. In this review, we provide a guide for exercise researchers regarding analytical techniques and experimental workflows commonly used in metabolomics and lipidomics. Furthermore, we discuss advancements in human and mammalian exercise research utilizing metabolomic and lipidomic approaches in the last decade, as well as highlight key technical considerations and remaining knowledge gaps to continue expanding the molecular landscape of exercise biology.

Keywordsexercise; metabolism; omics; metabolomics; metabolome; lipidomics; lipidome; mass spectrometry; nuclear magnetic resonance
Year2021
JournalMetabolites
Journal citation11 (151), p. Article 151
PublisherMultidisciplinary Digital Publishing Institute (MDPI AG)
ISSN2218-1989
Digital Object Identifier (DOI)https://doi.org/10.3390/metabo11030151
PubMed ID33799958
Scopus EID2-s2.0-85103051402
PubMed Central IDPMC8001908
Open accessPublished as ‘gold’ (paid) open access
Research or scholarlyResearch
Page range1-34
FunderAustralian Catholic University (ACU)
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online07 Mar 2021
Publication process dates
Accepted05 Mar 2021
Deposited04 Apr 2022
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