Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults

Journal article


Tatucu-Babet, Oana A., Forsyth, Adrienne, Owen, Emma, Navarro-Perez, Diana, Radcliffe, Jessica, Benheim, Devin, Mendis, Himasha, Jois, Markandeya, Itsiopoulos, Catherine and Tierney, Audrey C.. (2020). Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults. Nutrition Research. 78, pp. 82-92. https://doi.org/10.1016/j.nutres.2020.05.003
AuthorsTatucu-Babet, Oana A., Forsyth, Adrienne, Owen, Emma, Navarro-Perez, Diana, Radcliffe, Jessica, Benheim, Devin, Mendis, Himasha, Jois, Markandeya, Itsiopoulos, Catherine and Tierney, Audrey C.
Abstract

The association between intestinal permeability (IP) and body composition remains unclear. The gold standard differential sugar-absorption test is arduous to complete, with zonulin being increasingly used as an independent biomarker of IP. This pilot study aimed to explore the association between small IP, zonulin concentrations, and body composition in healthy adults. The urinary lactulose-rhamnose ratio was used to measure small IP. Serum zonulin, lipopolysaccharide (LPS) and high-sensitivity C-reactive protein (hs-CRP) were analyzed in serum. Body composition was measured using dual-energy X-ray absorptiometry and anthropometric measurements were collected. In total, 34 participants were included (12 males, median age 28 years, body mass index 24 kg/m2, waist circumference 77cm). No correlation was observed between the lactulose-rhamnose ratio and zonulin (r = -.016, P = .929). The lactulose-rhamnose ratio displayed a strong positive correlation with LPS (n 20, r = .536, P = .018) but did not correlate with body composition measures. Conversely, zonulin displayed a moderate positive correlation with waist circumference (r = .437, P = .042) in female participants and hs-CRP (r = .485, P = .004) in all participants. These findings raise important considerations for the measurement of small IP, warranting exploration in larger powered studies that address the limitations of the present study.

Keywordsintestinal permeability; L-R ratio; zonulin; endotoxemia; inflammation; body composition
Year2020
JournalNutrition Research
Journal citation78, pp. 82-92
PublisherElsevier Inc.
ISSN0271-5317
Digital Object Identifier (DOI)https://doi.org/10.1016/j.nutres.2020.05.003
Scopus EID2-s2.0-85086602041
Research or scholarlyResearch
Page range82-92
FunderThe Australasian Society of Parenteral and Enteral Nutrition
Research Training Program Scholarship, Australian Government
La Trobe University
Publisher's version
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All rights reserved
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Controlled
Output statusPublished
Publication dates
Online19 May 2020
Publication process dates
Accepted08 May 2020
Deposited23 Aug 2022
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