Mitochondria-targeted antioxidantsupplementation improves 8 km time trialperformance in middle-aged trained male cyclists

Journal article


Broome, S. C., Braakhuis, A. J., Mitchell, C. J. and Merry, T. L.. (2021). Mitochondria-targeted antioxidantsupplementation improves 8 km time trialperformance in middle-aged trained male cyclists. Journal of the International Society of Sports Nutrition. 18(1), p. Article 58. https://doi.org/10.1186/s12970-021-00454-0
AuthorsBroome, S. C., Braakhuis, A. J., Mitchell, C. J. and Merry, T. L.
Abstract

Background
Exercise increases skeletal muscle reactive oxygen species (ROS) production, which may contribute to the onset of muscular fatigue and impair athletic performance. Mitochondria-targeted antioxidants such as MitoQ, which contains a ubiquinone moiety and is targeted to mitochondria through the addition of a lipophilic triphenylphosphonium cation, are becoming popular amongst active individuals as they are designed to accumulate within mitochondria and may provide targeted protection against exercise-induced oxidative stress. However, the effect of MitoQ supplementation on cycling performance is currently unknown. Here, we investigate whether MitoQ supplementation can improve cycling performance measured as time to complete an 8 km time trial.

Method
In a randomized, double-blind, placebo-controlled crossover study, 19 middle-aged (age: 44 ± 4 years) recreationally trained (VO2peak: 58.5 ± 6.2 ml·kg− 1·min− 1, distance cycled per week during 6 months prior to study enrollment: 158.3 ± 58.4 km) male cyclists completed 45 min cycling at 70% VO2peak followed by an 8 km time trial after 28 days of supplementation with MitoQ (20 mg·day− 1) and a placebo. Free F2-isoprostanes were measured in plasma samples collected at rest, after 45 min cycling at 70% VO2peak and after completion of the time trial. Respiratory gases and measures of rating of perceived exertion (RPE) were also collected.

Results
Mean completion time for the time trial was 1.3% faster with MitoQ (12.91 ± 0.94 min) compared to placebo (13.09 ± 0.95 min, p = 0.04, 95% CI [0.05, 2.64], d = 0.2). There was no difference in RPE during the time trial between conditions (p = 0.82) despite there being a 4.4% increase in average power output during the time trial following MitoQ supplementation compared to placebo (placebo; 270 ± 51 W, MitoQ; 280 ± 53 W, p = 0.04, 95% CI [0.49, 8.22], d = 0.2). Plasma F2-isoprostanes were lower on completion of the time trial following MitoQ supplementation (35.89 ± 13.6 pg·ml− 1) compared to placebo (44.7 ± 16.9 pg·ml− 1 p = 0.03).

Conclusion
These data suggest that MitoQ supplementation may be an effective nutritional strategy to attenuate exercise-induced increases in oxidative damage to lipids and improve cycling performance.

KeywordsROS; mitochondria; antioxidant; performance; oxidative stress
Year2021
JournalJournal of the International Society of Sports Nutrition
Journal citation18 (1), p. Article 58
PublisherBioMed Central
ISSN1550-2783
Digital Object Identifier (DOI)https://doi.org/10.1186/s12970-021-00454-0
PubMed ID34419082
Scopus EID2-s2.0-85113269773
PubMed Central IDPMC8379793
Open accessPublished as ‘gold’ (paid) open access
Page range1-11
FunderCallaghan Innovation
MitoQ
Publisher's version
License
File Access Level
Open
Output statusPublished
Publication dates
Online21 Aug 2021
Publication process dates
Accepted21 Jun 2021
Deposited16 Jan 2023
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https://acuresearchbank.acu.edu.au/item/8y99q/mitochondria-targeted-antioxidantsupplementation-improves-8-km-time-trialperformance-in-middle-aged-trained-male-cyclists

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