β-Catenin is required for optimal exercise- andcontraction-stimulated skeletal muscle glucose uptake

Journal article


Masson, Stewart W. C., Woodhead, Jonathan S. T., D'Souza, Randall F., Broome, Sophie C., MacRae, Caitlin, Cho, Hyun C., Atiola, Robert D., Futi, Tumani, Dent, Jessica R., Shepherd, Peter R. and Merry, Troy L.. (2021). β-Catenin is required for optimal exercise- andcontraction-stimulated skeletal muscle glucose uptake. Journal of Physiology. 599(16), pp. 3897-3912. https://doi.org/10.1113/JP281352
AuthorsMasson, Stewart W. C., Woodhead, Jonathan S. T., D'Souza, Randall F., Broome, Sophie C., MacRae, Caitlin, Cho, Hyun C., Atiola, Robert D., Futi, Tumani, Dent, Jessica R., Shepherd, Peter R. and Merry, Troy L.
Abstract

The conserved structural protein β-catenin is an emerging regulator of vesicle trafficking in multiple tissues and supports insulin-stimulated glucose transporter 4 (GLUT4) translocation in skeletal muscle by facilitating cortical actin remodelling. Actin remodelling may be a convergence point between insulin and exercise/contraction-stimulated glucose uptake. Here we investigated whether β-catenin is involved in regulating exercise/contraction-stimulated glucose uptake. We report that the muscle-specific deletion of β-catenin induced in adult mice (BCAT-mKO) impairs both exercise- and contraction (isolated muscle)-induced glucose uptake without affecting running performance or canonical exercise signalling pathways. Furthermore, high intensity exercise in mice and contraction of myotubes and isolated muscles led to the phosphorylation of β-cateninS675, and this was impaired by Rac1 inhibition. Moderate intensity exercise in control and Rac1 muscle-specific knockout mice did not induce muscle β-cateninS675 phosphorylation, suggesting exercise intensity-dependent regulation of β-cateninS675. Introduction of a non-phosphorylatable S675A mutant of β-catenin into myoblasts impaired GLUT4 translocation and actin remodelling stimulated by carbachol, a Rac1 and RhoA activator. Exercise-induced increases in cross-sectional phalloidin staining (F-actin marker) of gastrocnemius muscle was impaired in muscle from BCAT-mKO mice. Collectively our findings suggest that β-catenin is required for optimal glucose transport in muscle during exercise/contraction, potentially via facilitating actin cytoskeleton remodelling.

Keywordsactin; glucose transport; GLUT4; insulin; Rac1
Year2021
JournalJournal of Physiology
Journal citation599 (16), pp. 3897-3912
PublisherWiley
ISSN0022-3751
Digital Object Identifier (DOI)https://doi.org/10.1113/JP281352
Scopus EID2-s2.0-85109790745
Page range3897-3912
FunderRutherford Discovery Fellowship
University of Auckland
Publisher's version
License
All rights reserved
File Access Level
Controlled
Output statusPublished
Publication dates
Online27 Jun 2021
Publication process dates
Accepted22 Jun 2021
Deposited17 Jan 2023
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