A probiotic mix partially protects against castration-induced bone loss in male mice
Journal article
Lawenius, Lina, Colldén, Hannah, Horkeby, Karin, Wu, Jianyao, Grahnemo, Louise, Vandenput, Liesbeth, Ohlsson, Claes and Sjögren, Klara. (2022). A probiotic mix partially protects against castration-induced bone loss in male mice. Journal of Endocrinology. 254(2), pp. 91-101. https://doi.org/10.1530/JOE-21-0408
Authors | Lawenius, Lina, Colldén, Hannah, Horkeby, Karin, Wu, Jianyao, Grahnemo, Louise, Vandenput, Liesbeth, Ohlsson, Claes and Sjögren, Klara |
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Abstract | Studies in postmenopausal women and ovariectomized mice show that the probiotic mix Lacticaseibacillus paracasei DSM13434, Lactiplantibacillus plantarum DSM 15312 and DSM 15313 (L. Mix) can protect from bone loss caused by sex steroid deficiency. Whether probiotic bacteria can protect bone also in sex steroid-deficient males is less studied. We used the orchiectomized mouse as a model for age-dependent bone loss caused by decreasing sex hormone levels in males. We treated 10-week-old male mice with either vehicle (veh) or L. Mix for 6 weeks, starting 2 weeks before orchiectomy (orx) or sham surgery. Importantly, mice treated with L. Mix had a general increase in total body bone mineral density (BMD) and lean mass (P ≤ 0.05) compared with veh-treated mice. Detailed computer tomography analysis of dissected bones showed increased trabecular BMD of the distal metaphyseal region of the femur in L. Mix compared to veh-treated orx mice (+8.0%, P ≤ 0.05). In the vertebra, L. Mix treatment increased trabecular bone volume fraction BV/TV (+11.5%, P ≤ 0.05) compared to veh in orx mice. Also, L. Mix increased the levels of short-chain fatty acids (SCFAs) such as propionate and acetate and important intermediates in SCFA synthesis such as succinate and lactate in the cecal content of male mice. In conclusion, L. Mix treatment resulted in a general increase in BMD in adult male mice and prevented trabecular bone loss in femur and vertebra of orx mice. These bone protective effects of L. Mix were associated with increased levels of SCFAs in the cecal content of male mice. |
Keywords | osteoporosis; bone mass; probiotic; gut microbiome; short-chain fatty acids |
Year | 2022 |
Journal | Journal of Endocrinology |
Journal citation | 254 (2), pp. 91-101 |
Publisher | BioScientifica Ltd. |
ISSN | 0022-0795 |
Digital Object Identifier (DOI) | https://doi.org/10.1530/JOE-21-0408 |
PubMed ID | 35661635 |
Scopus EID | 2-s2.0-85134676141 |
PubMed Central ID | PMC9254303 |
Open access | Published as ‘gold’ (paid) open access |
Page range | 91-101 |
Funder | Swedish Research Council |
Government of Sweden | |
Lundberg Foundation | |
Torsten Söderberg Foundation | |
Probi AB | |
Knut and Alice Wallenberg Foundation | |
Publisher's version | License File Access Level Open |
Output status | Published |
Publication dates | |
Online | 23 Jun 2022 |
Publication process dates | |
Deposited | 01 Mar 2023 |
Grant ID | 2018-02589 |
ALF Agreement 238261 | |
2017-0081 | |
M65115 | |
KAW 2015.0317 |
https://acuresearchbank.acu.edu.au/item/8yw29/a-probiotic-mix-partially-protects-against-castration-induced-bone-loss-in-male-mice
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Publisher's version
OA_Lawenius_2022_A_probiotic_mix_partially_protects_against.pdf | |
License: CC BY 4.0 | |
File access level: Open |
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