The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells
Joglekar, Mugdha V. Patil, Deepak Joglekar, Vinay M. Rao, Guduru Venkat Reddy, Nageshwar Duvvuru Mitnala, Sasikala Shouche, Yogesh Hardikar, Anandwardhan
Joglekar, Mugdha V.
Patil, Deepak
Joglekar, Vinay M.
Rao, Guduru Venkat
Reddy, Nageshwar Duvvuru
Mitnala, Sasikala
Shouche, Yogesh
Hardikar, Anandwardhan
Abstract
Epithelial-to-mesenchymal transition is a phenomenon necessary for embryonic development and also seen during certain pathological conditions. We show here for the first time that reduction in miR-30 family microRNAs, is responsible for mesenchymal transition of primary cultures of human pancreatic epithelial cells. We found that miR-30 family microRNAs target mesenchymal gene transcripts and maintain them in a translationally inactive state. Forced depletion using miR-30 family specific anti-miRs leads to mesenchymal transition while ectopic overexpression maintains the epithelial phenotype. We also show that miR-30 family microRNAs increase in abundance during differentiation of pancreatic islet-derived mesenchymal cells into hormone-producing islet-like cell aggregates. Our studies in human adult diseased pancreas also demonstrate that miR-30 family microRNAs are expressed at lower abundance in fibrotic lesions during pancreatitis. Together, our data confirm that miR-30 family microRNAs form a part of the regulatory signaling events involved in cellular response of pancreatic epithelial cells during mesenchymal transition.
Keywords
microRNA, miR-30, EMT, pancreas, islet
Date
2009
Type
Journal article
Journal
Islets
Book
Volume
1
Issue
2
Page Range
137-147