Loading...
Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation
Jin, Dongbin Loh, Khai Lee Shamekhi, Tima Ting, Yi Tian Lim Kam Sian, Terry C. C. Roest, James Ooi, Joshua D. Vivian, Julian Philip Faridi, Pouya
Jin, Dongbin
Loh, Khai Lee
Shamekhi, Tima
Ting, Yi Tian
Lim Kam Sian, Terry C. C.
Roest, James
Ooi, Joshua D.
Vivian, Julian Philip
Faridi, Pouya
Abstract
Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.
Keywords
Cancer, Cell lines Gene editing HLA Immunopeptidomics Lentivirus Transduction Immunotherapy Neoantigens, Gene editing, HLA, Immunopeptidomics, Lentivirus, Transduction, Immunotherapy, Neoantigens
Date
2023
Type
Book chapter
Journal
Book
Inflammation and Cancer : Methods and Protocols
Volume
Issue
Page Range
351-369
Article Number
ACU Department
Marketing and Communications
Research Office
Research Office
Collections
Relation URI
Event URL
Open Access Status
License
All rights reserved
File Access
Controlled
Notes
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.