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Striated muscle activator of rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by mir-628-5p
Russell, Andrew Wallace, M. A. Kalanon, M. Zacharewicz, Evelyn Gatta, P. A.D. Garnham, Andrew Lamon, Séverine
Russell, Andrew
Wallace, M. A.
Kalanon, M.
Zacharewicz, Evelyn
Gatta, P. A.D.
Garnham, Andrew
Lamon, Séverine
Abstract
Aim: The striated muscle activator of Rho signalling (STARS) is a muscle-specific actin-binding protein. The STARS signalling pathway is activated by resistance exercise and is anticipated to play a role in signal mechan-otransduction. Animal studies have reported a negative regulation of STARS signalling with age, but such regulation has not been investigated in humans. Methods: Ten young (18–30 years) and 10 older (60–75 years) subjects completed an acute bout of resistance exercise. Gene and protein expres-sion of members of the STARS signalling pathway and miRNA expression of a subset of miRNAs, predicted or known to target members of STARS signalling pathway, were measured in muscle biopsies collected pre-exer-cise and 2 h post-exercise. Results: For the first time, we report a significant downregulation of the STARS protein in older subjects. However, there was no effect of age on the magnitude of STARS activation in response to an acute bout of exer-cise. Finally, we established that miR-628-5p, a miRNA regulated by age and exercise, binds to the STARS 3’UTR to directly downregulate its tran-scription. Conclusion: This study describes for the first time the resistance exercise-induced regulation of STARS signalling in skeletal muscle from older humans and identifies a new miRNA involved in the transcriptional con-trol of STARS.
Keywords
Ageing, exercise, microRNA, skeletal muscle, striated muscle activator of Rho signalling.
Date
2017
Type
Journal article
Journal
Acta Physiologica
Book
Volume
220
Issue
2
Page Range
263-274
Article Number
ACU Department
Centre for Exercise and Nutrition
Faculty of Health Sciences
Faculty of Health Sciences
Collections
Relation URI
Source URL
Event URL
Open Access Status
Open access
License
CC BY-NC-ND 4.0
File Access
Open