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Deciphering hematopoietic stem cells in their niches: A critical appraisal of genetic models, lineage tracing, and imaging strategies
Joseph, Chacko Quach, Julie M. Walkley, Carl R. Lane, Steven W. Celso, Cristina Lo Purton, Louise E.
Joseph, Chacko
Quach, Julie M.
Walkley, Carl R.
Lane, Steven W.
Celso, Cristina Lo
Purton, Louise E.
Abstract
In recent years, technical developments in mouse genetics and imaging equipment have substantially advanced our understanding of hematopoietic stem cells (HSCs) and their niche. The availability of numerous Cre strains for targeting HSCs and microenvironmental cells provides extensive flexibility in experimental design, but it can also pose significant challenges due to strain-specific differences in cell specificity. Here we outline various genetic approaches for isolating, detecting, and ablating HSCs and niche components and provide a guide for advantages and caveats to consider. We also discuss opportunities and limitations presented by imaging technologies that allow investigation of HSC behavior in situ.
Keywords
Date
2013
Type
Journal article
Journal
Cell Stem Cell
Book
Volume
13
Issue
5
Page Range
520-533
Article Number
ACU Department
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Faculty of Health Sciences
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Relation URI
Source URL
Event URL
Open Access Status
License
File Access
Controlled