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Attenuated PGC-1α isoforms following endurance exercise with blood flow restriction
Conceição, Miguel Soares Chacon-Mikahil, Mara Patricia Traina Telles, Guilherme Defante Libardi, Cleiton Augusto Júnior, Edson Manoel Mendes Vechin, Felipe Cassaro Lugani de Andrade, André Luis Gáspari, Arthur Fernandes Brum, Patrícia Chakur Cavaglieri, Cláudia Regina
Conceição, Miguel Soares
Chacon-Mikahil, Mara Patricia Traina
Telles, Guilherme Defante
Libardi, Cleiton Augusto
Júnior, Edson Manoel Mendes
Vechin, Felipe Cassaro
Lugani de Andrade, André Luis
Gáspari, Arthur Fernandes
Brum, Patrícia Chakur
Cavaglieri, Cláudia Regina
Author
Conceição, Miguel Soares
Chacon-Mikahil, Mara Patricia Traina
Telles, Guilherme Defante
Libardi, Cleiton Augusto
Júnior, Edson Manoel Mendes
Vechin, Felipe Cassaro
Lugani de Andrade, André Luis
Gáspari, Arthur Fernandes
Brum, Patrícia Chakur
Cavaglieri, Cláudia Regina
Serag, Sara
Spiegelman, Bruce M.
Hawley, John A.
Camera, Donny M.
Chacon-Mikahil, Mara Patricia Traina
Telles, Guilherme Defante
Libardi, Cleiton Augusto
Júnior, Edson Manoel Mendes
Vechin, Felipe Cassaro
Lugani de Andrade, André Luis
Gáspari, Arthur Fernandes
Brum, Patrícia Chakur
Cavaglieri, Cláudia Regina
Serag, Sara
Spiegelman, Bruce M.
Hawley, John A.
Camera, Donny M.
Abstract
Introduction
Exercise performed with blood flow restriction simultaneously enhances the acute responses to both myogenic and mitochondrial pathways with roles in training adaptation. We investigated isoform-specific gene expression of the peroxisome proliferator-activated receptor gamma coactivator 1 and selected target genes and proteins regulating skeletal muscle training adaptation.
Methods
Nine healthy, untrained males participated in a randomized, counterbalanced, crossover design in which each subject completed a bout of low-intensity endurance exercise performed with blood flow restriction (15 min cycling at 40% of V˙O2peak, BFR-EE), endurance exercise (30 min cycling at 70% of V˙O2peak, EE), or resistance exercise (4 × 10 repetitions of leg press at 70% of one-repetition maximum) separated by at least 1 wk of recovery. A single resting muscle biopsy (vastus lateralis) was obtained 2 wk before the first exercise trial (rest) and 3 h after each bout.
Results
Total PGC-1α mRNA abundance, along with all four isoforms, increased above rest with EE only (P < 0.05) being higher than BFR-EE (P < 0.05). PGC-1α1, 2, and 4 were higher after EE compared with resistance exercise (P < 0.05). EE also increased vascular endothelial growth factor, Hif-1α, and MuRF-1 mRNA abundance above rest (P < 0.05), whereas COXIV mRNA expression increased with EE compared with BFR-EE (P < 0.05).
Conclusion
The attenuated expression of all four PGC-1α isoforms when EE is performed with blood flow restriction suggests this type of exercise provides an insufficient stimulus to activate the signaling pathways governing mitochondrial and angiogenesis responses observed with moderate- to high-intensity EE.
Keywords
mitochondrial biogenesis, cell signaling, skeletal muscle, adaptation, angiogenesis, high-intensity exercise
Date
2016
Type
Journal article
Journal
Medicine and Science in Sports and Exercise
Book
Volume
16
Issue
4809
Page Range
1699-1707
Article Number
ACU Department
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Non-faculty
Faculty of Health Sciences
Non-faculty
Collections
Relation URI
Source URL
Event URL
Open Access Status
Published as green open access
License
File Access
Controlled
Open
Open