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Gut bacteria are critical for optimal muscle function: A potential link with glucose homeostasis
Nay, Kevin Jollet, Maxence Goustard, Benedicte Baati, Narjes Vernus, Barbara Pontones, Maria Lefeuvre-Orfila, Luz Bendavid, Claude Rué, Olivier Mariadassou, Mahendra
Nay, Kevin
Jollet, Maxence
Goustard, Benedicte
Baati, Narjes
Vernus, Barbara
Pontones, Maria
Lefeuvre-Orfila, Luz
Bendavid, Claude
Rué, Olivier
Mariadassou, Mahendra
Abstract
Gut microbiota is involved in the development of several chronic diseases, including diabetes, obesity, and cancer, through its interactions with the host organs. It has been suggested that the cross talk between gut microbiota and skeletal muscle plays a role in different pathological conditions, such as intestinal chronic inflammation and cachexia. However, it remains unclear whether gut microbiota directly influences skeletal muscle function. In this work, we studied the impact of gut microbiota modulation on mice skeletal muscle function and investigated the underlying mechanisms. We determined the consequences of gut microbiota depletion after treatment with a mixture of a broad spectrum of antibiotics for 21 days and after 10 days of natural reseeding. We found that, in gut microbiota-depleted mice, running endurance was decreased, as well as the extensor digitorum longus muscle fatigue index in an ex vivo contractile test. Importantly, the muscle endurance capacity was efficiently normalized by natural reseeding. These endurance changes were not related to variation in muscle mass, fiber typology, or mitochondrial function. However, several pertinent glucose metabolism markers, such as ileum gene expression of short fatty acid chain and glucose transporters G protein-coupled receptor 41 and sodium-glucose cotransporter 1 and muscle glycogen level, paralleled the muscle endurance changes observed after treatment with antibiotics for 21 days and reseeding. Because glycogen is a key energetic substrate for prolonged exercise, modulating its muscle availability via gut microbiota represents one potent mechanism that can contribute to the gut microbiota-skeletal muscle axis. Taken together, our results strongly support the hypothesis that gut bacteria are required for host optimal skeletal muscle function.
Keywords
contractile properties, dysbiosis, maximal aerobic velocity, mitochondrial biogenesis, muscle fatigue
Date
2019
Type
Journal article
Journal
American Journal of Physiology: Endocrinology and Metabolism
Book
Volume
317
Issue
1
Page Range
158-171
Article Number
ACU Department
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Faculty of Health Sciences
Collections
Relation URI
Source URL
Event URL
Open Access Status
License
File Access
Controlled