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Evidence for the role of AMPK in regulating PGC-1 alpha expression and mitochondrial proteins in mouse epididymal adipose tissue
Wan, Zhongxiao Root-Mccaig, Jared Castellani, Laura Kemp, Bruce Steinberg, Gregory Wright, David
Wan, Zhongxiao
Root-Mccaig, Jared
Castellani, Laura
Kemp, Bruce
Steinberg, Gregory
Wright, David
Abstract
Objective: PGC-1α is a transcriptional co-activator and master regulator of mitochondrial biogenesis. While extensively studied in skeletal and cardiac muscle, recent findings suggest that white adipose tissue PGC-1α plays an important role in regulating glucose homeostasis. The purpose of the present investigation was to evaluate the role of AMPK in regulating PGC-1α and mitochondrial enzymes in mouse epididymal and inguinal subcutaneous adipose tissue. Methods: Mitochondrial protein content and norepinephrine and CL 316,243-induced PGC-1α mRNA expression were studied in mouse epididymal and inguinal adipose tissue from wild-type and AMPK β1−/− mice. Results: The protein content and phosphorylation of AMPKα was reduced in epididymal adipose tissue from AMPK β1−/− compared to WT mice, concomitant with decreases in PGC-1α and mitochondrial marker proteins. Norepinephrine and CL 316,243-mediated induction of PGC-1α were decreased in cultured epididymal adipose tissue from AMPK β1−/− relative to WT mice. In inguinal adipose tissue from AMPK β1−/− mice, mitochondrial marker protein content and norepinephrine and CL 316,243-mediated increases in PGC-1α were normal despite reductions in the content and phosphorylation of AMPKα. Conclusions: Norepinephrine- and CL 316,243-mediated induction of PGC-1α and mitochondrial protein expression is regulated by AMPK in epididymal, but not inguinal adipose tissue.
Keywords
Date
2014
Type
Journal article
Journal
Obesity: a resourch journal
Book
Volume
22
Issue
3
Page Range
730-738
Article Number
ACU Department
Faculty of Health Sciences
Collections
Relation URI
Source URL
Event URL
Open Access Status
License
File Access
Controlled