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Evaluation of the MAIIA dipstick test to detect recombinant human erythropoietin in plasma
Ashenden, Michael Sharpe, Ken Garnham, Andrew Gore, Christopher J.
Ashenden, Michael
Sharpe, Ken
Garnham, Andrew
Gore, Christopher J.
Abstract
Recently a novel technology, referred to as the ‘EPO WGA MAIIA’ test, has been developed by Swedish researchers to discriminate between endogenous and recombinant human erythropoietin. In contrast to existing electrophoretic methods that are used by antidoping laboratories, this dipstick-based technique is simple and fast. Moreover it can be applied to either blood or urine specimens. These characteristics could prove advantageous if the test were adopted by antidoping authorities to determine blood doping in sport. We evaluated the sensitivity of EPO WGA MAIIA to detect the presence of recombinant human erythropoietin ( rhEPO ) in some archived plasma specimens which had been collected from healthy, active subjects either 72 h or 96 h after a ‘microdose’ intravenous injection of rhEPO. Under these conditions the test had modest sensitivity to discriminate rhEPO, with only two of nine subjects exceeding an arbitrary cut-off 3.09 SDs beyond the expected population mean. Sensitivity was improved to five out of six subjects if positivity was assessed according to the subject's own previous values rather than a population-based threshold. We conclude that, with further refinement, the dipstick test may supplement existing antidoping tests.
Keywords
blood doping, recombinant human erythropoietin, MAIIA
Date
2012
Type
Journal article
Journal
Journal of Pharmaceutical and Biomedical Analysis
Book
Volume
67-68
Issue
Page Range
123-128
Article Number
ACU Department
Centre for Exercise and Nutrition
Faculty of Health Sciences
Faculty of Health Sciences
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Relation URI
Source URL
Event URL
Open Access Status
License
File Access
Controlled