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Synergy and oxygen adaptation for development of next-generation probiotics
Khan, Muhammad Tanweer Dwibedi, Chinmay Sundh, Daniel Pradhan, Meenakshi Kraft, Jamie D. Caesar, Robert Tremaroli, Valentina Lorentzon, Mattias Bäckhed, Fredrik
Khan, Muhammad Tanweer
Dwibedi, Chinmay
Sundh, Daniel
Pradhan, Meenakshi
Kraft, Jamie D.
Caesar, Robert
Tremaroli, Valentina
Lorentzon, Mattias
Bäckhed, Fredrik
Abstract
The human gut microbiota has gained interest as an environmental factor that may contribute to health or disease1. The development of next-generation probiotics is a promising strategy to modulate the gut microbiota and improve human health; however, several key candidate next-generation probiotics are strictly anaerobic2 and may require synergy with other bacteria for optimal growth. Faecalibacterium prausnitzii is a highly prevalent and abundant human gut bacterium associated with human health, but it has not yet been developed into probiotic formulations2. Here we describe the co-isolation of F. prausnitzii and Desulfovibrio piger, a sulfate-reducing bacterium, and their cross-feeding for growth and butyrate production. To produce a next-generation probiotic formulation, we adapted F. prausnitzii to tolerate oxygen exposure, and, in proof-of-concept studies, we demonstrate that the symbiotic product is tolerated by mice and humans (ClinicalTrials.gov identifier: NCT03728868) and is detected in the human gut in a subset of study participants. Our study describes a technology for the production of next-generation probiotics based on the adaptation of strictly anaerobic bacteria to tolerate oxygen exposures without a reduction in potential beneficial properties. Our technology may be used for the development of other strictly anaerobic strains as next-generation probiotics.
Keywords
Date
2023
Type
Journal article
Journal
Nature
Book
Volume
620
Issue
Page Range
381-385
Article Number
ACU Department
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Faculty of Health Sciences
Collections
Relation URI
Source URL
Event URL
Open Access Status
Published as ‘gold’ (paid) open access
License
CC BY 4.0
File Access
Open
Notes
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