Loading...
Collagen immunoassay as a method to optimise surface functionalisation
Stynes, Gil Kiroff, George Morrison, Wayne Kirkland, Mark
Stynes, Gil
Kiroff, George
Morrison, Wayne
Kirkland, Mark
Abstract
Traditional methods of assessing surface functionalisation, including spectroscopy and chemical labelling, often involve significant error and conjecture about bonds. Proteins that improve cell attachment have specific pKa's and optimum binding requirements that may differ from the conditions required for chemical labelling. The utility of collagen ELISA to optimise acetaldehyde glow discharge polymerisation reactor parameters was tested. Accurate stepwise increases in collagen conjugation strength were demonstrated by incubating specimens in 8 M urea for 5–8 days followed by ELISA to test for residual surface collagen. Surface modifications also were assessed by XPS. The results indicated that ELISA after bond-stressing with urea may suffice for optimising surface functionalisation and that traditional methods of analysis may be superfluous if protein conjugation is the aim of functionalisation.
Keywords
collagen, functionalisation of polymers, glow discharge polymerisation, immobilisation of molecules, surface modification
Date
2017
Type
Journal article
Journal
Plasma Processes and Polymers
Book
Volume
14
Issue
9
Page Range
1-9
Article Number
Article 1600213
ACU Department
School of Behavioural and Health Sciences
Faculty of Health Sciences
Faculty of Health Sciences
Relation URI
Source URL
Event URL
Open Access Status
License
All rights reserved
File Access
Controlled