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Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise
Camera, Donny M. Burniston, Jatin G. Pogson, Mark A. Smiles, William J. Hawley, John A.
Camera, Donny M.
Burniston, Jatin G.
Pogson, Mark A.
Smiles, William J.
Hawley, John A.
Abstract
It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction-induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP),we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein-by-protein basis inhuman skeletal muscle. Age-matched, overweight males consumed9 d of a high-fat, low-carbohydrate diet during which time they either undertook 3 sessions of REX or performed no exercise. Precursor enrichment and the rate of incorporation of deuterium oxide into newly synthesized muscle proteins were determined by mass spectrometry. Ninety proteins were included in the DPP, with28 proteins exhibiting significant responses to REX. The most common pattern of response was an increase in turnover, followed by an increase in abundance with no detectable increase in protein synthesis. Here, we provide novel evidence that demonstrates that the contribution of synthesis and breakdown to changes in protein abundance induced by REX differ on a protein by-protein basis. We also highlight the importance of the degradation of individual muscle proteins after exercise in human skeletal muscle.
Keywords
muscle protein synthesis, abundance, metabolism, adaptation, protein degradation
Date
2017
Type
Journal article
Journal
The FASEB Journal
Book
Volume
31
Issue
12
Page Range
5478-5494
Article Number
ACU Department
Non-faculty
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Collections
Relation URI
Source URL
Event URL
Open Access Status
Published as green open access
License
File Access
Controlled
Open
Open