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Dose and time effects of solarāsimulated ultraviolet radiation on the in vivo human skin transcriptome
M. Bustamante C. Hernandez-Ferrer A. Terwari Y. Sarria G. I. Harrison E. Puigdecanet L. Nonell W. Yang M.R. FriedlƤnder X. Estivill
M. Bustamante
C. Hernandez-Ferrer
A. Terwari
Y. Sarria
G. I. Harrison
E. Puigdecanet
L. Nonell
W. Yang
M.R. FriedlƤnder
X. Estivill
Abstract
Background
Terrestrial ultraviolet (UV) radiation causes erythema, oxidative stress, DNA mutations and skin cancer. Skin can adapt to these adverse effects by DNA repair, apoptosis, keratinization and tanning.
Objectives
To investigate the transcriptional response to fluorescent solarāsimulated radiation (FSSR) in sunāsensitive human skin in vivo.
Methods
Seven healthy male volunteers were exposed to 0, 3 and 6 standard erythemal doses (SED). Skin biopsies were taken at 6 h and 24 h after exposure. Gene and microRNA expression were quantified with next generation sequencing. A set of candidate genes was validated by quantitative polymerase chain reaction (qPCR); and wavelength dependence was examined in other volunteers through microarrays.
Results
The number of differentially expressed genes increased with FSSR dose and decreased between 6 and 24 h. Six hours after 6 SED, 4071 genes were differentially expressed, but only 16 genes were affected at 24 h after 3 SED. Genes for apoptosis and keratinization were prominent at 6 h, whereas inflammation and immunoregulation genes were predominant at 24 h. Validation by qPCR confirmed the altered expression of nine genes detected under all conditions; genes related to DNA repair and apoptosis; immunity and inflammation; pigmentation; and vitamin D synthesis. In general, candidate genes also responded to UVA1 (340ā400 nm) and/or UVB (300 nm), but with variations in wavelength dependence and peak expression time. Only four microRNAs were differentially expressed by FSSR.
Conclusions
The UV radiation doses of this acute study are readily achieved daily during holidays in the sun, suggesting that the skin transcriptional profile of ātypicalā holiday makers is markedly deregulated.
Keywords
Date
2020
Type
Journal article
Journal
British Journal of Dermatology
Book
Volume
182
Issue
6
Page Range
1458-1468
Article Number
ACU Department
Mary MacKillop Institute for Health Research
Faculty of Health Sciences
Faculty of Health Sciences
Collections
Relation URI
Source URL
Event URL
Open Access Status
Open access
License
CC BY-NC-ND 4.0
File Access
Open