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Tissue engineering by intrinsic vascularization in an in vivo tissue engineering chamber
Zhan, Weiqing Marre, Diego Mitchell, Geraldine M. Morrison, Wayne A. Lim, Shiang Y.
Zhan, Weiqing
Marre, Diego
Mitchell, Geraldine M.
Morrison, Wayne A.
Lim, Shiang Y.
Abstract
In reconstructive surgery, there is a clinical need for an alternative to the current methods of autologous reconstruction which are complex, costly and trade one defect for another. Tissue engineering holds the promise to address this increasing demand. However, most tissue engineering strategies fail to generate stable and functional tissue substitutes because of poor vascularization. This paper focuses on an in vivo tissue engineering chamber model of intrinsic vascularization where a perfused artery and a vein either as an arteriovenous loop or a flow-through pedicle configuration is directed inside a protected hollow chamber. In this chamber-based system angiogenic sprouting occurs from the arteriovenous vessels and this system attracts ischemic and inflammatory driven endogenous cell migration which gradually fills the chamber space with fibro-vascular tissue. Exogenous cell/matrix implantation at the time of chamber construction enhances cell survival and determines specificity of the engineered tissues which develop. Our studies have shown that this chamber model can successfully generate different tissues such as fat, cardiac muscle, liver and others. However, modifications and refinements are required to ensure target tissue formation is consistent and reproducible. This article describes a standardized protocol for the fabrication of two different vascularized tissue engineering chamber models in vivo.
Keywords
bioengineering, Issue 111, Tissue engineering, vascular pedicle, arteriovenous loop, vascularization, microsurgery, femoral vessel, angiogenesis, capillaries
Date
2016
Type
Journal article
Journal
Journal of Visualized Experiments
Book
Volume
2016
Issue
111
Page Range
1-7
Article Number
ACU Department
Collections
Relation URI
DOI
Source URL
Event URL
Open Access Status
License
File Access
Controlled